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Induction culture medium and induction culture method for unfertilized luffa ovaries

A technology of induction medium and culture method, which is applied in the field of induction medium and induction culture of loofah unfertilized cells, and can solve the problems that it is difficult to apply a general medium, and the study of in vitro gynogenesis induced haploid of loofah has not been reported. , to achieve the effect of improving induction efficiency and promoting embryonic development

Inactive Publication Date: 2019-09-27
杨泗海
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous research results have shown that it is difficult to find a universal medium that is suitable for each plant. It can only be screened for specific materials and environmental conditions, and then optimized on the basis of a large number of comparative analyzes
[0006] At present, haploids have been successfully obtained in cucumbers, melons, watermelons, and pumpkins through in vitro culture of unpollinated ovaries or ovules, but studies on the induction of haploids in loofah in vitro gynogenesis have not yet been reported.

Method used

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  • Induction culture medium and induction culture method for unfertilized luffa ovaries
  • Induction culture medium and induction culture method for unfertilized luffa ovaries
  • Induction culture medium and induction culture method for unfertilized luffa ovaries

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Experimental program
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Effect test

Embodiment 1

[0027] 1 Materials and methods

[0028] 1.1 Test material

[0029] Common loofah varieties 'Nanjing Snake-shaped loofah' and 'Wanlu 1' were selected as test materials, and the seeds of loofah were purchased from Nanjing Jiahua Agricultural Development Co., Ltd.

[0030] 1.2 Test method

[0031] (1) Cultivation of loofah plants: Sow the seeds of loofah in autumn from August to September for seedling cultivation. When the seedlings grow to three leaves and one heart, they will be planted for conventional cultivation and management.

[0032] (2) Collection and pretreatment of unfertilized ovaries: Bag the female flowers in the afternoon of the day before flowering to ensure that they are not pollinated; start sampling after the second female flower node on the day of flowering, and collect samples from healthy plants. unfertilized ovary without diseases and insect pests; the collected unfertilized ovary was brought back to the laboratory with a curling bottle, rinsed under runn...

Embodiment 2

[0059] According to the research results in Example 1, the best sponge gourd unfertilized ovary induction medium of the present invention is: KNO 3 1750mg / L, NH 4 NO 3 1610mg / L, MgSO 4 ·7H 2 O 225mg / L, KH 2 PO 4 345mg / L, calcium gluconate 450mg / L, Na 2 -EDTA 37.5mg / L, FeSO 4 ·7H 2 O 27.8mg / L, H 3 BO 3 12.6mg / L, KI 0.75mg / L, MnSO 4 4H 2 O22.4mg / L, ZnSO 4 ·7H 2 O 5.5mg / L, Na 2 MoO 4 2H 2 O 0.25mg / L, CuSO 4 ·5H 2 O 0.025mg / L, CoCl 2 ·6H 2 O 0.025mg / L, inositol 100mg / L, niacin 1.6mg / L, pyridoxine hydrochloride 0.9mg / L, thiamine hydrochloride 0.7mg / L, glycine 2.5mg / L, proline 32mg / L, Hydrolyzed milk protein 300mg / L, calcium pantothenate 0.7mg / L, TDZ 0.08mg / L, 6-BA 1.0mg / L, NAA 0.5mg / L, spermine 12.5mg / L, N-acetyl-5-methoxy Base tryptamine 0.2mg / L, diethyl sulfate 1.2mg / L, amino acid 20mg / L, loofah wound fluid 150ml / L, sucrose 40g / L, plant gel 6g / L, pH value 5.8.

[0060] The best way to induce culture of loofah unfertilized ovary is:

[0061] (1) Cultivatio...

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Abstract

The invention provides an induction culture medium for unfertilized luffa ovaries. A formula of the culture medium comprises major elements such as KNO3, NH4NO3 and calcium citrate, ferric salts, complex compounds, trace elements such as H3BO3 and KI, inositol, nicotinic acid, pyridoxine hydrochloride, thiamine hydrochloride, glycine, arginine, proline, hydrolyzed milk protein, calcium pantothenate, TDZ, 6-BA, NAA, spermine, diethyl sulfate, N-acetyl-5-methoxytryptamine, aminoxyacetic acid, bleeding sap of luffa, sucrose and plant gel. The invention also provides an induction culture method of the unfertilized luffa ovaries. After pretreatment and disinfection, the collected unfertilized luffa ovaries are inoculated onto the induction culture medium for culture, the average embryoid induction rate can reach 24.89%, and meanwhile, the growth conditions of obtained embryoids are great. The culture medium lays a foundation for establishing an in-vitro unfertilized luffa ovary culture system and carrying out luffa haploid breeding research.

Description

technical field [0001] The invention relates to an induction culture medium and an induction cultivation method of loofah unfertilized ovaries, and belongs to the technical field of vegetable cultivation. Background technique [0002] Loofah is an annual climbing herb of Cucurbitaceae. Loofah originated in tropical Asia. Since it was introduced into my country in the Song and Ming Dynasties, after a long period of natural selection and evolution, a total of 9 different species have been found so far, among which the common loofah ( Luffa cylindrica (L.)Roem.) and ribbed loofah ( Luffa acutangula (L.) Roxb.) is the main cultivated species in my country. Loofah is eaten as a tender fruit. The flesh of the loofah is delicate and soft, and it tastes delicious. Because of its high yield and good quality, it is widely planted in the north and south of my country. Loofah is rich in nutrition and has high nutritional and health value. According to measurements, every 100g loof...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 杨泗海
Owner 杨泗海
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