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Extraction method of allelochemical substance resisting microalgae from ruppia maritima and application of allelochemical substance

A kind of technology of cyanobacteria and substances, which is applied in the field of cyanobacteria bloom prevention and control, can solve the problems of inapplicability and nutrient salt entering the water body, and achieve the effects of avoiding nutrient backflow, high use efficiency, and high ecological security

Pending Publication Date: 2019-09-24
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Directly planting aquatic plants will release allelochemicals through the water environment to inhibit the growth of algae, but this method cannot be applied in seasons or water quality when aquatic plants cannot grow
Moreover, if the aquatic plants fail to be salvaged in time, nutrients will repeatedly enter the water body

Method used

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  • Extraction method of allelochemical substance resisting microalgae from ruppia maritima and application of allelochemical substance
  • Extraction method of allelochemical substance resisting microalgae from ruppia maritima and application of allelochemical substance
  • Extraction method of allelochemical substance resisting microalgae from ruppia maritima and application of allelochemical substance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] A method for extracting anti-microalgae allelochemicals from Siberian algae:

[0048] (1) Dried algae at 45°C for 48 hours, manually or mechanically crushed to pass through a 40-mesh sieve, added petroleum ether, algae: petroleum ether = 1:10 (m:v), left to extract for 24 hours, repeated 3 times , combine the extracts, filter with filter paper first, and then filter with a 0.22µm glass fiber filter membrane. The filtrate is evaporated under reduced pressure with a rotary evaporator at 45°C and 100r / min to obtain an extract. Substances, extract 1g and then dissolved in 10mL dimethyl sulfoxide organic solution, configured to a fixed concentration, is the mother solution of allelochemical substances;

[0049] (2) Add the allelochemical mother liquor to the medium containing Microcystis aeruginosa, the final concentration of the allelochemical added is 0, 25, 50, 100, 150, 200 mg / L, and the algae density is 5.5×10 5 cell / mL; the culture medium of described Microcystis aeru...

Embodiment 2

[0055] Allelochemicals were tested to inhibit algae at different concentrations, the concentration gradient was set to 0, 25, 50, 100, 150, 200 mg / L, and the algae density range was 1×10 5 -1×10 6 cell / mL. algae growth curve figure 1 . Among them, the concentration of petroleum ether extract of Chuanmania algae was 200 mg / L, the seven-day inhibition rate reached 92.88%, and the seven-day half-inhibition rate concentration was 92.82 mg L -1 . After placing the allelochemicals for 30 days, the seven-day inhibition rate was 14.91% at a concentration of 200 mg / L. The biological activity was reduced by 83.95%. It shows that this allelochemical substance can be degraded naturally and will not cause damage to the ecological environment.

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Abstract

The invention discloses an extraction method of an allelochemical substance resisting microalgae from ruppia maritima. Petroleum ether is adopted as a solvent, the allelochemical substance is extracted from the ruppia maritima, during extraction, the extraction operation is conducted on the ruppia maritima three times by adopting the petroleum ether at 15-30 DEG C according to the ratio of 1:10 (m:v), after the extraction operation, organic phase materials are mixed, through pressure reduction and concentration, an organic solvent is volatilized, and the allelochemical substance of the ruppia maritima is obtained; after dilution, the allelochemical substance is applied to algal inhibition. It is shown through an experiment result that the allelochemical substance extracted from the ruppia maritima by adopting the petroleum ether has an efficient inhibiting effect on microcystis aeruginosa, the addition quantity of the allelochemical substance is 90-200 mg / L, the rate of inhibiting the microcystis aeruginosa is 50-95%, and under the condition of the inhibiting rate after seven and a half days, the concentration of the allelochemical substance is 92 mg / L. After 30 days, the allelochemical substance can be naturally degraded and cannot cause secondary pollution to a water environment. The extraction method has the advantage of being simple, high in ecological safety and the like, and provides the scientific basis for inhibiting methods for cyanobacterial bloom by utilizing the allelochemical substance in the water environment protection field.

Description

technical field [0001] The invention belongs to the technical field of prevention and control of cyanobacteria blooms, and in particular relates to a method and application of extracting anti-microalgae allelochemicals from Siberian algae. Background technique [0002] Plant allelopathy refers to the harmful or beneficial effects of plants (microorganisms) on other plants (microorganisms) by releasing allelochemical substances into the environment. [0003] Since the 1980s, the eutrophication of many water bodies has led to the outbreak of algal blooms. For example, Taihu Lake, Chaohu Lake, Dianchi Lake, Dongting Lake and the Three Gorges Reservoir have been infested by algal blooms, and even caused serious drinking water supply crises. Microcystis aeruginosa belongs to cyanobacteria, which is a kind of dominant algae that forms water bloom. The cyanobacteria bloom will cover a large area of ​​the surface of the water body, reduce the dissolved oxygen in the water body, wea...

Claims

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Application Information

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IPC IPC(8): C07C39/16C07C37/82A01N65/40A01N31/08A01P13/00
CPCC07C39/16C07C37/004C07C37/82A01N65/00A01N65/40A01N31/08
Inventor 卢学强张欣
Owner NANKAI UNIV
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