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Preparation method of immobilized microbial agent for kitchen waste treatment and application thereof

A technology for immobilizing microorganisms and kitchen waste, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc. The effect is not strong and other problems, to achieve the effect of preventing a large loss of live bacteria, combining tightly, and strong ability

Active Publication Date: 2019-09-20
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Although the above-mentioned patent applications all provide the microbial strain formula of the food waste degrading bacteria agent, none of the above-mentioned strains involves the problem of eliminating the peculiar smell in the process of degrading the food waste, resulting in a very obvious odor of the degradation product
[0010] In addition, although the above-mentioned patent applications all add carriers, they all adopt the preparation method of directly mixing the bacteria with the carrier, resulting in the inclose contact between the bacteria and the carrier, and the protective effect on the bacteria is not strong, which is not strict. immobilized bacterial agent

Method used

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  • Preparation method of immobilized microbial agent for kitchen waste treatment and application thereof
  • Preparation method of immobilized microbial agent for kitchen waste treatment and application thereof
  • Preparation method of immobilized microbial agent for kitchen waste treatment and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Screening and Identification of Predominant Bacteria for Degrading Food Waste

[0045] Take 5g of the soil sample near the Yuxiu Restaurant of Zhejiang University of Technology and put it into a sterilized Erlenmeyer flask with small glass beads and 50mL of sterile water, shake at 30°C and 200r / min for 20min, and suspend the bacteria in the ultra-clean bench. Liquid gradient dilution, the concentration gradient is 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 , inoculate 100 μL of each concentration of bacterial suspension on a PDA plate, seal the plate with a sealing film and invert it, place it in a constant temperature incubator at 28-37°C for 24-48 hours, select a plate with a suitable number of colonies, and pick typical colonies , after separation and purification, inoculate them on protein, starch, fat, and cellulose differential medium plates and spread evenly with a sterilized coating rod. Seal the culture dish with a parafilm and place it ups...

Embodiment 2

[0069] The preparation of embodiment 2 microbial bacterial agents

[0070] 1. Preparation of unimmobilized microbial agents

[0071] (1) Preparation of composite bacteria

[0072] (1) Incline cultivation:

[0073] Pichia kluyveri ZJB-091 and Geotrichumsilvicola ZJB-092 were inoculated on PDA solid medium respectively;

[0074] Among them, Geotrichum linsheng was cultured at a temperature of 30° C. for 48 hours, and Pichia kluyveri was cultured at a temperature of 28° C. for 72 hours to activate the strains.

[0075] (2) Primary seed liquid culture:

[0076] The activated Pichia kluyveri and Geotrichum forestiata after the activation of step (1) are inoculated respectively in 250mL liquid culture medium, carry out seed culture, obtain Pichia kluyveri first grade seed liquid culture and Geotrichum forestiata grade one seed liquid culture;

[0077] Among them, Geotrichum linsheng was cultured at a temperature of 30° C. for 48 hours, and Pichia kluyveri was cultured at a temp...

Embodiment 3

[0103] This embodiment uses the different immobilized microbial agents prepared in Example 2 to perform a degradation test on kitchen waste:

[0104] (1) Take the leftover meals in the school cafeteria and sort them to remove solid waste such as bones, paper scraps, wooden chopsticks, and plastic;

[0105] (2) Add 15kg of sorted kitchen waste in the garbage disposer, get the microbial inoculum that embodiment 2 makes (respectively unimmobilized microbial inoculum, immobilized microbial inoculum 1 and immobilized microbial inoculum Agent II) 900g is put into the garbage disposer, after continuing to ferment for 24 hours, then add 10kg of the above-mentioned food waste every other day, for 10 consecutive days, the weight ratio of the total food waste added to the microbial agent is about 130:1 ;

[0106] The fermentation temperature is 55°C, and the fermentation time is 28 hours; during the fermentation process, stirring is carried out, and the stirring frequency is 5 minutes e...

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Abstract

The invention discloses a preparation method of an immobilized microbial agent for kitchen waste treatment and an application thereof. The method comprises the following steps: respectively performing high-density liquid fermentation on Kluyer pichia yeast and geotrichum silvicola, and mixing the bacterial liquids in proportion; after concentrating a composite bacterial solution, adding the composite bacterial solution to a modified carrier, mixing the materials, adding a crosslinking agent during a mixing process, and then performing constant temperature aeration culture; and after constant temperature aeration, performing low temperature drying to obtain the immobilized microbial agent. The method of the invention not only significantly increases the amount of carrier immobilized thalline, but also makes the binding between the carrier and the thalline tight, prevents the living bacteria from being lost in the preparation process and the preservation, improves the live bacteria of the unit mass of the microbial agent, and reduces the amount of the microbial agent; The prepared microbial agent has strong capability for decomposing the kitchen waste, the odor generated in the process of degrading the kitchen waste can be eliminated, the amount of the thalline immobilized in the carrier is large, the binding between the carrier and the thalline is closer, and the biomass of the living bacteria is large.

Description

technical field [0001] The invention relates to the field of environmental protection biotechnology, in particular to a preparation method and application of an immobilized microbial agent for treating kitchen waste. Background technique [0002] Compared with free microorganisms, microorganisms immobilized in the carrier space can not only maintain good activity and stability, but also help cells resist the influence of adverse environments such as acidity and alkalinity, high osmotic pressure and high temperature. Moreover, cell immobilization technology can increase the concentration of microorganisms in a certain space. The cell immobilization technology has been widely valued by researchers for its advantages of simple method, reusable, high stability and easy separation. Today, when advocating environmental protection, cell immobilization technology has been applied to sewage treatment, which makes water resources well recycled; in the pharmaceutical industry, cell im...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/12C12N11/10C12N11/08B09B3/00B09B5/00C12R1/84C12R1/645
CPCC12N11/12C12N11/10C12N11/08B09B5/00B09B3/40
Inventor 郑裕国刘奇薛亚平邹树平柯霞周海岩柳志强
Owner ZHEJIANG UNIV OF TECH
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