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Application of robenidine hydrochloride in preparation of medicine for treating fungal infection

A technology of prohexidine hydrochloride and fungal infection, which is applied in the direction of antifungal agents, drug combinations, pharmaceutical formulas, etc., to achieve the effects of low toxicity, good killing effect, and remarkable antibacterial effect

Active Publication Date: 2019-09-20
SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Chlorhexidine hydrochloride, as a kind of anti-coccidiosis veterinary drug, is widely used in chicken and rabbit breeding, and studies have shown that the residual dose of this drug in edible tissues is reliable, safe and has no side effects on the human body. The research results of the drug's inhibitory effect on fungi were published

Method used

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  • Application of robenidine hydrochloride in preparation of medicine for treating fungal infection
  • Application of robenidine hydrochloride in preparation of medicine for treating fungal infection
  • Application of robenidine hydrochloride in preparation of medicine for treating fungal infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Experimental situation of the growth of Candida albicans after being treated with fluconazole and chlorhexidine hydrochloride respectively.

[0039] Use a sterile inoculation loop to take a small piece of strain from the frozen Candida albicans strain and inoculate it into a solid medium containing YPD, and incubate overnight (15 hours) in a 30°C incubator; after incubation overnight, wash with sterile PBS Twice; use a microplate reader to measure the concentration of the bacterial solution, and insert it into the YPD medium to make it OD 600 =0.4; Dissolve the prohexidine hydrochloride and fluconazole dissolved in DMSO in fresh YPD medium respectively, and configure to the required concentration, make 2-fold dilution in a 96-well plate, and then add 100 μl before Add 200μl to each well of the culture medium containing the bacteria solution, so that the concentration of the bacteria solution is OD 600 =0.2, the drug concentration from low to high is 1μM, 2μM, 4μM, 8μM,...

Embodiment 2

[0042] Antibacterial effect of prohexidine hydrochloride on clinical fluconazole-resistant strains and Candida auris

[0043] The experiment process is the same as in Example 1, except that the test strains are replaced by drug-resistant strains and Candida auris.

[0044] Experimental results such as figure 2 As shown, compared with the fluconazole control group, Chlorhexidine hydrochloride showed a significant effect on two drug-resistant bacteria #16 and #17 (Candida albicans drug-resistant strains extracted from clinically treated tumor patients). Obvious antibacterial effect, and in cells treated with different concentrations, prohexidine hydrochloride has a dose-dependent effect, and the inhibitory effect on cells is gradually enhanced from low to high. In addition, this experiment also added Candida auris, by figure 2 D in D can be obtained, and prohexidine hydrochloride also has a strong inhibitory effect on Candida auris, which has good clinical significance for t...

Embodiment 3

[0047] Chlorhexidine hydrochloride has significant antibacterial effect on several common human pathogenic fungi such as Saccharomyces cerevisiae, Cryptococcus neoformans and Aspergillus fumigatus.

[0048] The experimental process is the same as in Example 1, except that the test strains are replaced by Saccharomyces cerevisiae, Cryptococcus neoformans, and Aspergillus fumigatus.

[0049] Experimental results such as image 3 As shown, the growth curves of Saccharomyces cerevisiae and Candida albicans are very similar, and the minimum inhibitory concentration is 4 μM; the dose-effect relationship between Aspergillus fumigatus and Cryptococcus neoformans is obvious, and the growth of Candida albicans can be completely inhibited at the concentration of 32 μM (growth curve levels within 48 hours). Through the above experiments, it can be shown that prohexidine hydrochloride has a good inhibitory effect on these common clinical pathogenic fungi.

[0050] image 3 A is the anti...

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Abstract

The invention belongs to the field of medicine preparation, and particularly relates to application of robenidine hydrochloride in preparation of medicine for treating fungal infection. Experiments prove that the minimum bacteriostatic concentration of the robenidine hydrochloride is 4 mu M, and is lower than that of fluconazole which is a common antifungal drug, the bacteriostatic effect of the robenidine hydrochloride is more obvious at the same concentration dose, the robenidine hydrochloride still has a good killing effect on drug-resistant fungal strains and super fungi, the toxicity is low, and the robenidine hydrochloride is safe and reliable. The invention also finds that the cell wall and the transcription factor RLM1 are drug targets of robenidine hydrochloride for treating fungal infection, and provides broad prospects for drug treatment of fungal infection.

Description

technical field [0001] The invention belongs to the field of medicine preparation, in particular to the application of prohexidine hydrochloride in the preparation of medicines for treating fungal infections. Background technique [0002] Fungal infection has a huge impact on human health. Every year, billions of people around the world suffer from fungal infection, causing more than 1.5 million deaths. With the development of medical science, the use of various new drugs and technologies such as broad-spectrum antibiotics, glucocorticoids, immunosuppressants, various catheterization techniques, organ, bone marrow transplantation, etc. is becoming more and more common. For example, malignant tumors, HIV infection, and aging population are also increasing day by day. The morbidity and mortality of opportunistic pathogenic fungal infections caused by this are increasing year by year, and have been paid more and more attention by the medical field. Existing antifungal drugs ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/155A61P31/10A61P17/00
CPCA61K31/155A61P17/00A61P31/10
Inventor 王慧刘宁宁
Owner SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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