Electrochemical detection method of capsaicins in illegal cooking oil

A detection method, waste oil technology, applied in the field of electrochemical detection, can solve the problems of complicated instrument maintenance, complicated antibody preparation, and cross-reaction, and achieve the effects of low production cost, efficient quantitative detection, and simple production process

Active Publication Date: 2019-09-17
CHONGQING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Liquid chromatography-mass spectrometry has the advantages of efficient separation, high sensitivity, and high accuracy, but the instrument is expensive, the operation process is complicated, and the maintenance of the instrument is complicated, so it is difficult to popularize and apply
Immunoassay method, low sensitivity, complicated antibody preparation, and cross-reaction problems
[0004]Although there have been reports on the detection of capsaicinoids by electrochemical methods at home and abroad, there has been no use of AuNPs / Fe3O4Related research reports on quantification of capsaicinoids in waste oil by electrochemical method combined with nanocomposite materials and azo reaction

Method used

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  • Electrochemical detection method of capsaicins in illegal cooking oil
  • Electrochemical detection method of capsaicins in illegal cooking oil
  • Electrochemical detection method of capsaicins in illegal cooking oil

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Embodiment 1

[0055] The present embodiment proposes the electrochemical detection method of capsaicinoids described in a kind of waste oil, specifically comprises the following steps:

[0056] (1) AuNPs / Fe 3 o 4 Synthesis of Nanocomposites:

[0057] Magnetic Fe 3 o 4 Nanoparticles dispersed in HAuCl containing 0.05mmol / L 4 , 125.0mmol / L isopropanol and 10.0mg / mL polyvinyl alcohol aqueous solution, the formation of 1.0mg / mL Fe 3 o 4 A mixed solution of nanoparticles; ultrasonically disperse the mixed solution for 20min with an ultrasonic power of 120W, place it in a glass reagent bottle, and irradiate it with a high-energy electron beam of 10.0MeV at a dose of 6kGy to obtain uniformly dispersed AuNPs / Fe 3 o 4 Nanocomposite materials, and then stored in a refrigerator at 4°C, protected from light;

[0058] (2) AuNPs / Fe 3 o 4 - Preparation of ATP-azo-Caps complex:

[0059] a. The AuNPs / Fe prepared in the above step (1) 3 o 4 Mix the nanocomposite material with an equal volume o...

Embodiment 2

[0069] In this embodiment, the AuNPs / Fe 3 o 4 The morphology of nanocomposites was characterized, and the results are shown in figure 2 . Gold nanoparticles successfully encapsulated in magnetic Fe 3 o 4 particle surface, the complex has a large specific surface area, and the magnetic Fe 3 o 4 Particle stability and dispersibility are enhanced.

Embodiment 3

[0071] DPV detection method was used to investigate the electrochemical behavior of capsaicinoids, the results are shown in image 3 . Under the same concentration (0.50ng / mL), the electrochemical detection of capsaicin (dotted line a) and dihydrocapsaicin (short horizontal line b) was carried out, and it was found that the two had the same oxidation peak potential and similar Oxidation peak current. After mixing capsaicin and dihydrocapsaicin aqueous solution (solid line c), it was found that the oxidation peak potential was unchanged, and the oxidation peak current was approximately the sum of the peak currents when the two were detected separately. Therefore, it is speculated that capsaicinoids have the same electrochemical behavior. Therefore, capsaicin was used as a representative to establish and evaluate the electrochemical detection method of capsaicinoids.

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Abstract

The invention discloses an electrochemical detection method of capsaicins in illegal cooking oil. The method comprises the following steps: adopting gold-nano-particles / magnetic ferroferric oxide (AuNPs / Fe3O4)nano composite material, immobilizing 4-aminothiophenol (4-ATP) through an Au-S bond, enabling the capsaicins (Caps) obtained by performing liquid-liquid extraction on the illegal cooking oil to couple with the AuNPs / Fe3O4-ATP after diazotization, thereby forming the AuNPs / Fe3O4-ATP-azo-Caps compound, and enriching the compound on a surface of the silk screen printed carbon electrode working electrode by utilizing an external magnetic field. The electrochemical detection is performed by adopting a differential pulse voltammetry, and the sensitive, efficient, accurate and quantitative detection on the Caps in the illegal cooking oil sample is realized according to a linear relation between the electrochemical response signal and the to-be-detected substance concentration, thereby providing technical support for the identification of the illegal cooking oil.

Description

technical field [0001] The invention belongs to the field of electrochemical detection, in particular to a sensitive and efficient electrochemical detection method for capsaicinoids in waste oil. Background technique [0002] Waste oil (ICO) is a general term for various low-quality oils. The swill oil from the catering industry is its main raw material, and it also includes frying waste oil. Nutrients such as essential fatty acids and phytosterols in edible oil are lost in large quantities during the refining process, and harmful components such as mycotoxins remain in waste oil, and regular consumption of waste oil will cause serious harm to human health. Capsaicinoids (Caps) are more or less added condiments to three meals a day. Because of their lipophilicity, they become active ingredients that inevitably exist in waste oil and can be used for the identification of waste oil. Capsaicinoids are the active ingredients obtained from capsicum fruits, which are characterize...

Claims

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Application Information

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IPC IPC(8): G01N27/48G01N27/30
CPCG01N27/48G01N27/30
Inventor 丁敏蒋梦丹张晓清罗娟吕文静谭杰峰
Owner CHONGQING MEDICAL UNIVERSITY
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