Somatic cell culturing method for effectively promoting mongolian oak globular embryogeny

A culturing method and somatic cell technology are applied in the field of somatic cell culturing for effectively promoting globular embryogenesis of Quercus mongolica, which can solve the problems of being susceptible to a variety of insects, long growth cycle and low reproduction coefficient, and achieve high callus induction. The effect of high somatic embryo induction rate, dense and dense cytoplasm

Inactive Publication Date: 2019-09-17
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the severe shortage of fine varieties of Quercus mongolica, the long growth cycle, late fruiting period, and low yield, the seeds appear in a big year every 3-5 years. In addition, the seeds are not resistant to storage and are easily attacked by various insects during collection and storage. All these characteristics make it difficult to establish a Quercus mongolica seed orchard
Although conventional asexual reproduction methods such as cuttings and grafting can maintain the excellent traits of the female parent for a long time, it is difficult for Quercus mongolica to take root, the reproduction coefficient is low, and the speed is slow.

Method used

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  • Somatic cell culturing method for effectively promoting mongolian oak globular embryogeny
  • Somatic cell culturing method for effectively promoting mongolian oak globular embryogeny
  • Somatic cell culturing method for effectively promoting mongolian oak globular embryogeny

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Experimental program
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Effect test

Embodiment 1

[0038] 1. Test material

[0039] Sampling was carried out every 15 days from June 25 to September 10, 2017 and 2018 in the campus of Shenyang Agricultural University and Wandianzi Forest Farm in Qingyuan County, Fushun. The immature and mature seeds of Quercus mongolica with shells were packed in plastic bags and brought back to the laboratory, and stored in a refrigerator at 4°C for future use.

[0040] 2. A method for somatic cell culture that effectively promotes Quercus mongolica globular embryogenesis, comprising the following steps:

[0041] (1) Selection and treatment of explants: select seeds that are free from pests and plump, peel off the outer shells and husks of the seeds, wash them under running water for 1 hour, and then move them to the aseptic workbench;

[0042] (2) Disinfection of explants: Disinfect the seeds treated in step (1) with 75% alcohol for 30 seconds, rinse them with sterile water for 3 times, then disinfect them with 0.1% mercury liter for 12 min...

Embodiment 2

[0062] 1. Test material

[0063] In mid-January 2018, the stored Quercus mongolica seeds were sown indoors (natural light, room temperature 16°C), and germination began in about 20 days. After about 2 months, the samples were collected every 15 days from mid-March to early May.

[0064] 2. A method for somatic cell culture that effectively promotes Quercus mongolica globular embryogenesis, comprising the following steps:

[0065] (1) Selection and processing of leaf explants: collect young leaves from the first round of growth point on the top of Quercus mongolica seedlings with a seedling age of 70-75d, and place the collected young leaves under running water for 1 hour and then move them to a free place. Bacteria workbench;

[0066] (2) Disinfection of explants: Disinfect the young leaves after step (1) with 75% alcohol for 30 seconds, rinse them with sterile water for 3 times, then disinfect them with 0.1% mercuric chloride for 4 minutes, and rinse them with sterile water ...

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Abstract

The invention provides a somatic cell culturing method for effectively promoting mongolian oak globular embryogeny. The method is characterized by comprising the following steps of selection and treatment of an explant, disinfection for the explant, inoculation, callus induction and somatic embryo induction. The somatic cell culturing method for effectively promoting mongolian oak globular embryogeny has the following advantages that the rate of callus induction and the somatic embryo induction rate are high, and embryonic callus with a small cell volume, compact and thick cytoplasm and a large and clear cell nucleus is obtained; the problems are solved that cutting rooting of mongolian oak is difficult, the propagation coefficient is small, the speed is low, and large-scale production cannot be formed for meeting the market requirements, and by utilizing the cell totipotency, the purpose of large-scale cultivation of good plants in a short period is achieved; the possibility is provided for establishment of the asexual propagation system of the mongolian oak and industrialized seedling culturing of the mongolian oak.

Description

technical field [0001] The invention belongs to the technical field of plant cell culture, and in particular relates to a somatic cell culture method for effectively promoting globular embryogenesis of Quercus mongolica. Background technique [0002] Quercus mongolica (Quercus mongolica) is a plant of the genus Quercus (Fagaceae), also known as Quercus mongolica, Oak oak, Oak tree, and belongs to deciduous trees. It is a national second-class precious tree species in China and the main secondary forest tree species in the forest regions of Northeast China. Produced in Heilongjiang, Jilin, Liaoning, Inner Mongolia, Hebei and other provinces and regions in China. It is also distributed in Russia, North Korea, and Japan, and it is planted in many places in the world. Because of its tall tree shape, Quercus mongolica can be used as a garden tree or street tree, and those with good tree shape can be used as solitary trees for ornamental purposes. Quercus mongolica seeds have h...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 陆秀君李前张丽杰梅梅张晓林王傲艾万峰战昊韩晓义李美莹金子涵
Owner SHENYANG AGRI UNIV
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