A kind of detection method of microorganism in water environment

A water sample and indicator technology, applied in the field of microorganisms, can solve the problems of complex operation process, low sensitivity, complex instruments, etc., and achieve the effects of not easy to fade, large economic benefits, and excellent effects.

Active Publication Date: 2020-08-04
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing problems of these technologies are that the operation process is relatively complicated, complex instruments are required, the time is long, and the sensitivity is not high.

Method used

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  • A kind of detection method of microorganism in water environment
  • A kind of detection method of microorganism in water environment
  • A kind of detection method of microorganism in water environment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Preparation of concentrated corn steep liquor particles:

[0028] Use a refiner to grind corn kernels into corn steep liquor. During the refining process, the mass ratio of corn kernels to water is 4:1. The ground corn syrup is first evaporated at 40°C to obtain corn steep liquor with a concentration of 40%. The material is then evaporated in a heating cycle at 80°C to obtain a basic dry product, which is dried at 90°C.

[0029] The final dried product is ground to a particle size of 100-500 nanometers, which is corn steep liquor concentrated particles.

[0030] (2) Dyed protein powder and corn steep liquor

[0031] Add 5 parts of protein powder, 3 parts of corn steep liquor granules and 30 parts of water into the reaction kettle, heat up to 60°C, stir evenly at 1000rpm, then slowly add 10mmol / L sodium persulfate dropwise at a rate of 1mL / min, add safflower 3 parts of dye, after the dropwise addition is completed, stir for 2 hours, let stand, and cool down to norm...

Embodiment 2

[0036] (1) Preparation of concentrated corn steep liquor particles:

[0037] Use a refiner to grind corn kernels into corn slurry. During the refining process, the mass ratio of corn kernels to water is 6:1. Evaporate the ground corn slurry, first at 50°C, to obtain a concentration of 50%. The corn slurry is then evaporated by heating cycle at 90°C to obtain a basic dry product, which is then dried at 100°C.

[0038] The final dried product is ground to obtain particles with a particle size of about 500 nanometers, which are corn steep liquor concentrated particles.

[0039] (2) Dyed protein powder and corn steep liquor

[0040] Add 8 parts of protein powder and 4 parts of corn steep liquor granules, 40 parts of water into the reaction kettle, heat up to 70°C, 2000rpm, stir evenly, then slowly add 10mmol / L sodium persulfate at a rate of 2mL / min, add safflower dye 3 parts, after the dropwise addition is completed, stir for 2 hours, let stand, and cool down to normal temperatu...

Embodiment 3

[0045] (1) Preparation of concentrated corn steep liquor particles:

[0046] Use a refiner to grind corn kernels into corn slurry. During the refining process, the mass ratio of corn kernels to water is 7:1. Evaporate the ground corn slurry, first at 60°C, to obtain a concentration of 50%. The corn slurry is then evaporated by heating cycle at 90°C to obtain a basic dry product, which is then dried at 100°C.

[0047] The final dried product is ground to obtain particles with a particle size of about 1000 nanometers, which are corn steep liquor concentrated particles.

[0048] (2) Dyed protein powder and corn steep liquor

[0049]Add 10 parts of protein powder, 5 parts of corn steep liquor granules, 50 parts of water into the reaction kettle, heat up to 80°C, 3000rpm, stir evenly, then slowly add 10mmol / L potassium persulfate at a rate of 3mL / min, add safflower dye 3 parts, after the dropwise addition is completed, stir for 2 hours, let stand, and cool down to normal temperat...

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Abstract

The invention discloses a detection method of microorganisms in water environment, and belongs to the technical field of microbial technology. More specifically, the invention provides an indicator and a detection method for detecting number of microorganisms in water environment. The water-quality microorganism detection method utilizes natural substances, such as corn, proteins, safflower and the like; and thus, the water-quality microorganism detection method is ecological, environmentally friendly and free of pollution to the water quality. The water-quality microorganism detection methodprovided by the invention is convenient to use and high in efficiency, and has an accuracy higher than 90%; moreover, the water-quality microorganism detection method is capable of saving medium culture time, namely to obtain results within 2 hour, and is low in detection supply cost. Detection results can be obtained in a very short time by utilizing the water-quality microorganism detection method.

Description

technical field [0001] The invention relates to a method for detecting microorganisms in a water environment, belonging to the technical field of microorganisms. Background technique [0002] Water body is a good solvent, which dissolves inorganic nutrients such as nitrogen, phosphorus, and sulfur, and organic matter that enters water in various forms. Generally speaking, although the content of various nutrients in water body is not as rich as that of soil, it can basically Meet the needs of microbial growth and development; the temperature of various water bodies has a large difference, and there is a large change with the season; the pH range of different water bodies is 3.7 to 10.5, most of which are 6.5 to 8.5, suitable for the growth of most microorganisms, while Corresponding microbial groups also grow in some acidic and alkaline water bodies. In addition, due to the low solubility of oxygen in water, it is easily depleted by microorganisms, so the survival of aquati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/04
CPCC12Q1/04
Inventor 张丹倪永浩郑磊
Owner JIANGNAN UNIV
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