A method for determining the interaction efficiency of quinones and carboxymethyl lysine in solution

Abstract

The invention discloses a method for measuring the interaction efficiency of quinone substances and carboxymethyl lysine (CML) in solution. The method measures the cyclic voltammetry curves of the catechols solution before and after the addition of CML, records the peak current and voltage characteristic values ​​of the catechols solution before and after the addition of CML, and then calculates the reduction rate of the reduction peak current by calculating , the interaction efficiency between quinones in solution and CML can be obtained. In the method of the invention, after the phenolic substances are oxidized to form quinones, they can quickly interact with the CML in the system to reduce the side reactions of the quinones; the amount of substances attached to the electrode surface is small, and the detection accuracy is high; In the prior art, the adhesion of polyphenols and their oxidation products to the working electrode, the related side reactions of quinones and other factors interfere with the interaction between quinones and CML; at the same time, the assay method of the invention is simple, the analysis time is short, and the reproducibility is good. and convenience, low cost of use, and wide application.

Description

technical field [0001] The invention relates to the technical field of food safety detection and analytical chemistry, and more particularly, to a method for determining the interaction efficiency of quinones and carboxymethyl lysine (CML) in solution. Background technique [0002] CML (carboxymethyl lysine) is a typical advanced glycation end product (AGEs) that is most widely studied at present, and it exists widely in various foods. CML can accumulate in tissues after being digested and absorbed, and existing studies have proved its potential harm to human health. Therefore, research on the means to reduce the content of CML can effectively reduce its potential harm to human health. [0003] While catechols play an antioxidant role, they will be oxidized to form corresponding o-phthaloquinones. As strong electrophiles, o-phthaloquinones are prone to Michael addition reaction with amino groups on CML. So as to achieve the purpose of eliminating the system CML. At presen...

AI Technical Summary

Problems solved by technology

Therefore, it is impossible to use existing means to measure the interaction between the quinones obtained from the oxidation of the above-mentioned polyphenols in the solution and the CML.

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