Construction and application of prediction model for effective components of alfalfa hay
A technology of alfalfa hay and active ingredients is applied in the directions of measuring devices, analyzing materials, and material analysis by optical means, which can solve the problems of low economic benefit and waste of alfalfa hay resources, and achieves low detection cost, saving of feeding cost, Effects of no-reagent drug consumption
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Embodiment 1
[0040] Embodiment 1: Utilize the chemical analysis method to analyze the main components of alfalfa hay
[0041] 1. Pretreatment of alfalfa hay samples: put the alfalfa hay into an electric blast drying oven, and dry it in an oven at 105°C until the moisture content is reduced to about 2-10%, then use a high-speed pulverizer to crush the alfalfa hay, and then separate the samples with Sieve and sieve the dried samples, take 40-mesh whole plant silage samples and store them in ziplock bags, and carry out subsequent steps after balancing the moisture for 24 hours.
[0042] 2. Determination of moisture content: put the clean weighing bottle in an oven at 105°C, remove the cap of the weighing bottle and place it on the side of the weighing bottle. After baking for 30 minutes, cover the weighing bottle cap, take out the weighing bottle, put it in a desiccator to cool for 30 minutes, and weigh its mass (m 1 ), accurate to 1mg.
[0043] Weigh 2~5g sample (m 2 ) in the weighing bot...
Embodiment 2
[0095] Example 2: Near-infrared spectrum collection of alfalfa hay
[0096] Put the alfalfa hay sample with a particle size of 40 mesh into a sample cup with a diameter of 9.4cm and a depth of 4.8cm. No gaps. The near-infrared spectrum of the alfalfa hay sample was collected in the wavelength range of 1400nm-2500nm by using the grating continuous spectrum mode of the Spectrastar 1400XL-3 near-infrared spectrometer of Unity Company of the United States. The spectral collection rate is 45 degs / sec, the wavelength accuracy is 1nm, and the sample is scanned repeatedly for 3 times;
[0097] The main components of alfalfa hay are crude protein, water, neutral detergent fiber, acid detergent fiber, and crude ash, all of which have strong absorption in the near-infrared region.
[0098] Such as figure 1 As shown, the main components of alfalfa hay have a strong absorption peak at 1950nm, and the absorption trends of all samples are the same, and they all show the same shape absorpt...
Embodiment 3
[0099] Example 3: Verification test on the correlation between the screening of near-infrared spectroscopy pretreatment methods and the main components of alfalfa hay
[0100]Select 265 representative samples from various places in Henan Province, detect and analyze the content of each active ingredient according to the method described in Example 1, and collect the near-infrared spectra of each sample according to the method in Example 2.
[0101] The Ucal data analysis software of Unity Company in the United States was used to perform derivative processing, detrend correction (Detrend) and standard normal variable transformation (SNV) preprocessing on the diffuse reflectance raw spectra of the obtained alfalfa hay samples.
[0102] The spectrogram after detrend correction (Detrend) and standard normal variable transformation (SNV) preprocessing is as follows figure 2 shown.
[0103] On the basis of a large amount of data accumulation combined with long-term practical exper...
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