Method for improving yield of vitamin B12 based on regulation of ammonia nitrogen index

Abstract

Provided is a method for improving the yield of vitamin B12 based on regulation of an ammonia nitrogen index. In the fermentation process of vitamin B12, stage-by-stage batch feeding is carried out; in the earlier stage of fermentation, a yeast extract juice is refilled, so a certain content of ammonia nitrogen is maintained at a certain stage, so as to ensure the nitrogen source needed for growthand metabolism of bacteria. The stage-by-stage batch feeding is carried out in the fermentation process, the yeast extract juice is refilled in the earlier stage of fermentation, the concentration ofammonia nitrogen is controlled, the contradiction between the growth of the bacteria and biosynthesis of high-concentration ammonia nitrogen in the fermentation of vitamin B12 is solved, the nutritional components required for the growth and metabolism of the bacteria are ensured in the early stage of fermentation, the growth of bacteria is promoted, the phenomena of premature senescence and metabolic stagnation in the later period are avoided, and the fermentation unit of vitamin B12 is increased.

Description

technical field [0001] The invention relates to a method for improving vitamin B 12 The method of yield, especially relates to a kind of regulation and control based on ammonia nitrogen index to improve vitamin B 12 yield method. Background technique [0002] B Vitamins in Nature 12 All are synthesized by microorganisms, and higher animals and plants cannot produce vitamin B 12 . Vitamin B 12 Obtained through strain cultivation and fermentation. Vitamin B 12 Its main physiological function is to participate in the manufacture of bone marrow red blood cells, prevent pernicious anemia, and prevent brain nerves from being damaged. [0003] Vitamin B present 12 The production of aerobic sword bacteria (Ensifer adhaerens) is obtained through fermentation and purification. The main components of the fermentation medium include: organic nitrogen source, carbon source, betaine, precursors and some inorganic salts and trace elements. The main function of the nitrogen source ...

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems of the prior art, the present invention provides a method for improving vitamin B vitamins based on ammonia nitrogen index regulation and control. 12 The method of the yield, the method of the present invention carries out staged feeding in batches during the fermentation process, and yeast leach juice is added in the early stage of fermentation to control the ammonia nitrogen content, and solve the problem of high concentration ammonia nitrogen in vitamin B 12 The contradiction between bacterial growth and biosynthesis during fermentation ensures the nutrients needed for bacterial growth and metabolism in the early stage of fermentation, promotes the growth of bacterial cells, avoids the phenomenon of premature aging of bacterial cells and stagnation of metabolism in the later stage, and thus increases vitamin B. 12 fermentation unit

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