Cordyceps cicadae polysaccharide, preparation method and application thereof in preparation of medicament for preventing renal interstitial fibrosis

Abstract

The invention discloses a cordyceps cicadae polysaccharide, a preparation method and application thereof in preparation of a medicament for preventing renal interstitial fibrosis. The method comprisesthe following steps: pulverizing cordyceps cicadae fruiting bodies, and performing sieving to obtain cordyceps cicadae fruiting body powder; ultrasonically extracting the fruiting body powder with distilled water, and then carrying out ultrahigh pressure treatment; preparing crude polysaccharide of the cordyceps cicadae fruiting body after the treatment; and then performing separation by using DEAE-cellulose anion column chromatography, and performing freeze-drying to obtain the purified polysaccharide. The method successfully prepares the cicada cordyceps polysaccharide treated and modifiedby ultrahigh pressure, and has the advantages of simple steps and higher efficiency. The intervention on a rat glomerular epithelial cell model shows that the polysaccharide can effectively inhibit the proliferation of rat glomerular epithelial cells (HMC), and has wide application prospect in the aspect of preventing renal interstitial fibrosis. The oxidation resistance of the cordyceps cicadae polysaccharide is obviously improved, and the cordyceps cicadae polysaccharide induces the apoptosis of high-glucose rat glomerular mesangial cells, has no toxicity to normal cells, and has good research value and commercial value.

Description

technical field [0001] The present invention relates to a preparation method and application of an entomogenous fungus, in particular to a kind of polysaccharide of Cicadae sinensis, preparation method and application in preparation of medicine for preventing renal interstitial fibrosis. Background technique [0002] Cordyceps cicadae (Cordyceps cicadae), also known as golden cicada, is a dry complex parasitic on cicada nymphs by Paecilomyces cicadae, a fungus of the genus Cordyceps ergotaceae. one. The recorded history of Cordyceps sinensis is 800 years earlier than Cordyceps sinensis, and some active ingredients are higher than Cordyceps sinensis. Modern medical research shows that Cordyceps cicadae contains a large amount of functional components such as cordycepic acid, cordyceps polysaccharide, adenosine, a variety of alkaloids and ergosterol, which can improve kidney function, relieve chronic renal failure, regulate lipid metabolism, relieve fever and analgesia, Seda...

AI Technical Summary

Problems solved by technology

However, the research on Cordyceps cicadae is not deep at home and abroad, and there are fewer varieties of artificial cultivation and selection, and they often focus on the research on the activity of cicadae mycelium.

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