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Combined detection kit and detection method for viruses associated with respiratory infection nucleic acid

A joint detection and viral nucleic acid technology, applied in the biological field, can solve the problems of joint detection of respiratory syncytial virus without influenza A virus and influenza B virus, and achieve the effects of convenient new drug development, pollution reduction, and easy operation

Pending Publication Date: 2019-08-27
SHANGHAI XINGYAO MED TECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Currently, there are no kits for the combined detection of influenza A virus, influenza B virus, and respiratory syncytial virus

Method used

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  • Combined detection kit and detection method for viruses associated with respiratory infection nucleic acid
  • Combined detection kit and detection method for viruses associated with respiratory infection nucleic acid
  • Combined detection kit and detection method for viruses associated with respiratory infection nucleic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] Embodiment 1 A kind of respiratory virus nucleic acid combined detection kit

[0079] (1) Extraction of respiratory virus nucleic acid

[0080] The nucleic acid extraction and purification reagents produced by Shanghai Fosun Changzheng Medical Science Co., Ltd. were used to extract nucleic acid from human nasopharyngeal swab samples.

[0081] (2) Fluorescent RT-PCR amplification

[0082] a. Add sample:

[0083] Add 20 μL of nucleic acid extraction solution to the detection tube containing 30 μL of reaction solution, and the reaction volume is 50 μL;

[0084] b. One-step RT-PCR reaction procedure:

[0085]

[0086] c. Collect fluorescence signals of FAM, HEX, ROX and CY5 channels in the eighth step.

[0087] (3) Detection: the present invention is applicable to ABI 7500 fluorescent quantitative PCR instrument for virus nucleic acid identification and detection.

[0088] (4) Result judgment:

[0089] A. Quality control:

[0090] The Ct values ​​detected by the C...

Embodiment 2

[0097] Embodiment 2 clinical detection

[0098] Using the method above, 74 cases of clinical samples were qualitatively detected by fluorescent PCR, including 18 patients with influenza A virus (including 1 patient who was positive for both influenza A virus and respiratory syncytial virus). figure 1 16 cases of influenza A virus patients were detected by the colloidal gold detection method commonly used in clinical practice; the influenza A / B nucleic acid detection kit (fluorescent PCR method) produced by Shuoshi Biotechnology Co., Ltd., which has been listed, (Cat. No. : JC10202N) The fluorescent PCR confirmation test was performed on the 2 samples with inconsistent detection results by the two methods: both samples were positive for influenza A virus (see figure 2 ), showing that the detection efficiency of the inventive method is high; 21 cases of patients with influenza B virus, the fluorescence detection results are shown in image 3 100% (21 / 21) coincidence rate with ...

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Abstract

The invention discloses a combined detection kit and detection method for viruses associated with respiratory infection nucleic acid, which have the advantages of high specificity, high sensitivity, simple operation, clear result and high reliability, and can be used for qualitative identification and detection of influenza A virus, influenza B virus and respiratory syncytial virus.

Description

technical field [0001] The invention relates to the field of biotechnology, and more specifically relates to a combined detection kit and detection method for respiratory virus nucleic acid. Background technique [0002] Respiratory viruses (Viruses associated with respiratory infections) refer to the general term for viruses that mainly invade the respiratory tract and first proliferate in the epithelial cells of the respiratory tract mucosa, causing respiratory and systemic infections, and causing damage to the respiratory tract and other organs. 90-95% of clinical acute respiratory infections are caused by this group of viruses. Respiratory viruses are mostly single-stranded RNA (ssRNA) viruses, generally with envelopes and spikes, mainly transmitted through the respiratory tract, localized in the respiratory tract, can be repeatedly infected, and have a high prevalence rate in the population. Among them, influenza virus (influenza virus) and respiratory syncytial virus ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/686C12R1/93
CPCC12Q1/701C12Q1/686C12Q2600/166C12Q2600/16C12Q2521/107C12Q2521/531C12Q2531/113C12Q2537/143C12Q2545/101C12Q2561/101
Inventor 迟大利杨志军夏懿吴大治
Owner SHANGHAI XINGYAO MED TECH DEV CO LTD
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