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Malaria vaccine

A malaria and vaccine technology, applied in the direction of allergic diseases, immunoglobulin, and vector-borne diseases, can solve the problems of unused malaria vaccines

Pending Publication Date: 2019-08-23
SUMITOMO DAINIPPON PHARMA CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although malaria vaccines have been researched or are being developed worldwide, malaria vaccines are not yet in clinical use

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0126] Use a vector containing the codon-optimized sequence of wheat encoding Ripr linked at the C-terminus to the coding sequence of the His tag, namely pEU-E01-MCS; The vector of the sequence of one of the cloned 11 fragments, pEU-E01-GST-TEV-N2, served as a template for transcription. All peptides were synthesized using templates containing the Met coding sequence at the N-terminus and the 6xHis coding sequence at the C-terminus.

[0127] Encoding SEQ ID NO: 21 ~ 1086 amino acids, SEQ ID NO: 2 amino acids 720 ~ 934 (SEQ ID NO: 4), and SEQ ID NO: 2 amino acids 648 ~ 830 (SEQ ID The wheat codon-optimized sequences of NO: 8) are shown in SEQ ID NO: 5, SEQ ID NO: 6, and SEQ ID NO: 9, respectively. Totally transcribed mRNA was used for protein synthesis using wheat germ cell-free protein synthesis kit WEPRO®7240H (cell free Science). The resulting solution containing the synthetic protein was affinity purified by using Ni Sepharose 6 Fast Flow (GE Healthcare). The antigen sol...

Embodiment 2

[0148] The baculovirus codon-optimized sequence encoding Ripr1-5 (SEQ ID NO: 10) linked at the N-terminus to the Gp67 secretion signal coding sequence and at the C-terminus to the His-tag coding sequence was subcloned into the pFastBac1 vector. By using the obtained expression vector and DH10Bac competent cells, recombinant bacmids were prepared. The recombinant bacmid was transfected into Sf9 insect cells with Cellfectin II reagent to produce recombinant baculovirus. The recombinant baculovirus was amplified and used to infect Sf9 insect cells to express Ripr1-5. The culture supernatant was collected, and Ripr1-5 was purified by Ni-NTA affinity column and Superdex200 gel filtration column.

[0149] In the same manner as in Example 1, a rabbit polyclonal antibody was obtained by using Ripr1-5 (Bac-Ripr1-5) produced using the baculovirus / insect cell expression system. The inhibitory activity of the rabbit polyclonal antibody against the proliferation of Plasmodium was compare...

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Abstract

The present invention provides a polypeptide and a malaria vaccine comprising the polypeptide, the polypeptide comprising any of the following amino acid sequences: (a) an amino acid sequence represented by SEQ ID NO. 4 or SEQ ID NO. 8; (b) an amino acid sequence represented by SEQ ID NO. 4 or SEQ ID NO. 8, in which 1-10, preferably 1-5, and more preferably 1-3 amino acids have been substituted, deleted, added or inserted; and (c) an amino acid sequence having at least 95%, preferably at least 97%, and more preferably at least 99% sequence identity with SEQ ID NO. 4 or SEQ ID NO. 8.

Description

technical field [0001] This application claims priority from Japanese Patent Application Nos. 2016-220512 and 2017-161442, which are hereby incorporated by reference in their entirety. [0002] The present invention relates to vaccine antigens for use, for example, in the prevention of Plasmodium infection or in the prevention of the onset of malaria. Background technique [0003] Malaria is an infection by parasitic protozoa of the genus Plasmodium, such as Plasmodium falciparum, that is widespread in tropical and subtropical regions. Malaria infection occurs when Plasmodium enters the human body by using Anopheles mosquito as a carrier, and proliferates through a sporozoite stage, a liver stage, and a red blood cell stage. In various stages, the malaria parasite produces proteins in the human body. Vaccines that induce antibodies to this protein are thus expected to attack Plasmodium, or to inhibit infection by Plasmodium or proliferation in vivo following infection. Al...

Claims

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Application Information

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IPC IPC(8): C12N15/09A61K39/015A61K39/13C07K14/445C07K16/20C07K17/02C12N1/19C12N1/21C12N5/10A61K39/05A61K39/08A61K39/10A61P33/06A61P37/04A61P43/00
CPCA61K39/015A61P33/06A61P37/04A61P43/00C07K14/445Y02A50/30C07K16/205C07K2317/76A61K39/05A61K39/13C12N15/1006
Inventor 福岛晃久坪井敬文高岛英造长冈光
Owner SUMITOMO DAINIPPON PHARMA CO LTD
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