A screening method for immunoactive peptides

Abstract

The present invention relates to a screening method for immunologically active peptides. The screening method comprises the following steps: S1, hydrolyzing at least one protein containing immunologically active peptides by a hydrolase to obtain a peptide library composed of immunologically active peptides, wherein the peptide library is The peptide sequence of the peptide was obtained by mass spectrometry; S2, to screen out the peptide sequence with strong binding to MHC class II molecules; S3, to synthesize the peptide sequence from S2; S4, to construct an LPS-induced cell model to verify the synthesis of S3 The immune activity of the polypeptides. The invention can avoid the cumbersome identification of the peptide sequence and the binding site, and directly obtain the polypeptide bound to the MHC class II molecule; and can simultaneously explain the reactogenicity and immunogenicity of the polypeptide combined with the MHC class II molecule, so that the data is more accurate. Perfect, the process of obtaining peptides is faster and more efficient.

Description

technical field [0001] The present invention relates to the field of biotechnology, and more particularly, to a screening method for immunoactive peptides. Background technique [0002] In recent years, the phenomenon of various diseases caused by the decline of the body's immunity caused by aging, obesity, decreased sleep time or quality, smoking and drinking and other bad habits has become more and more serious. It has become an urgent need for people to seek suitable immunomodulators to improve human immunity. At present, the method to obtain immunologically active peptides is mainly separation and purification technology, and the means to obtain immunologically active peptides by using online prediction methods combined with experimental screening are very limited. The current separation and purification technology mainly separates the peptides in the peptide library into different components based on various molecular characteristics such as molecular weight, ionic str...

AI Technical Summary

Problems solved by technology

These methods have too many separated components, the experimental process is cumbersome, and the screening efficiency of active peptides is greatly reduced.

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