Screening method for immunoactive peptides

Abstract

The invention relates to a screening method for immunoactive peptides. The screening method comprises the following steps of S1, hydrolyzing at least one protein containing the immunoactive peptides through hydrolase in order to obtain a peptide library formed by the immunoactive peptide, wherein peptide sequences of polypeptides in the peptide library are obtained through a mass spectrometry technology; S2, screening out the peptide sequences strongly bound with MHC class II molecules; S3, synthesizing the peptide sequences screened out in S2; and S4, constructing an LPS-induced cell model for verifying the immune activity of the polypeptides synthesized by S3. According to the method, the complex identification of the subsequent peptide sequences and binding sites can be avoided, and thepolypeptides combined with the MHC class II molecules can be directly obtained; and furthermore, the reactogenicity and the immunogenicity of binding of the polypeptides and MHC class II molecules can be simultaneously described, so that data is more complete, a process of obtaining the polypeptides is more rapid, and the efficiency is higher.

Description

technical field [0001] The invention relates to the field of biotechnology, and more specifically, to a screening method for immunoactive peptides. Background technique [0002] In recent years, aging, obesity, decreased sleep time or quality, smoking and drinking and other unhealthy living habits lead to decreased immunity and various diseases. Seeking suitable immunomodulators to improve human immunity has become an urgent need for people. At present, the methods for obtaining immunoactive peptides are mainly separation and purification technology, but the means of obtaining immunoactive peptides by using online prediction methods combined with experimental screening are very limited. The current separation and purification technology mainly separates the peptides in the peptide library into different components according to various molecular characteristics such as molecular weight, ionic strength, and hydrophobicity, and then evaluates the components by immune indicator...

AI Technical Summary

Problems solved by technology

These methods have too many separated components, the experimental process is cumbersome, and the screening efficiency of active peptides is greatly reduced.

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