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A drug carrier system capable of targeted drug delivery in cancer cells and preparation method thereof

A technology of targeted drug delivery and carrier system, which is applied in the field of drug carrier system with the ability of targeted drug delivery in cancer cells and its preparation, which can solve hypoxia, reduce the effective oxygen concentration in PDT, and the imbalance between oxygen supply and oxygen consumption And other issues

Active Publication Date: 2021-07-06
HUBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unfortunately, aberrant angiogenesis and poor blood flow within solid tumors can lead to an imbalance between oxygen supply and consumption, resulting in high levels of hypoxia
The hypoxic environment of tumor tissue may reduce the concentration of available oxygen in PDT and limit the generation of ROS

Method used

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  • A drug carrier system capable of targeted drug delivery in cancer cells and preparation method thereof
  • A drug carrier system capable of targeted drug delivery in cancer cells and preparation method thereof
  • A drug carrier system capable of targeted drug delivery in cancer cells and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] A method of preparing a composite nano drug carrier system, including the steps of:

[0066] Synthetic PB-CD-NH 2

[0067] The PB 200 mg was scattered in PBS of 100 mL of pH of 5.5, and then the ice water bath was 4 ° C, EDC (1- (3-dimethylaminopropyl) -3-ethyl carbide hydrochloride 2.87 G and N-hydroxy succinimide 1.7262 g of reaction for 24 hours; then adding 140 mg-β-Cd and 60 mg of cystylene metalline salts for 24 hours, centrifugation, washed the resulting solid product, which is PB-CD-NH. 2 .

[0068] 2. Synthesis PB-C-DOTS-CD

[0069] The carbon quantum point 60 mg was scattered in PBS of 100 mL of a pH of 5.5, and then EDC1.7262G and N-hydroxy succinimide were added at 4 ° C for 24 hours, and then 200 mg Pb-CD-NH2 was added. The reaction was cleaned at room temperature for 24 h, and the resulting solid product was washed, which was PB-C-DOTS-CD.

[0070] 3. Synthesize PLL (NF)

[0071] 200 mg of PLL, 100 mg of potassium carbonate and 5-chloro-2-nithenyl trifluorome...

Embodiment 2

[0089] A method of preparing a composite nano drug carrier system, including the steps of:

[0090] Synthetic PB-CD-NH 2

[0091] It is weighing PB 200 mg to disperse in 100 ml of deionized water, and pH is adjusted to 5-6 with hydrochloric acid (if it is adjusted below 5, a small amount of sodium hydroxide is adjusted), and then the ice water bath is 4 ° C, add EDC (1- (1-) 3-dimethylaminopropyl) -3-ethyl carbon diimide hydrochloride 2.87 g of N-hydroxy succinimide 1.7262 g, reaction 24 hours; then add 140 mgeda-β-Cd and 60 mg cysteamine salts The acid salt reaction was 24 hours, and the obtained solid product was centrifuged, that is, PB-CD-NH2.

[0092] 2. Synthesis PB-C-DOTS-CD

[0093] The carbon quantum dot was scattered in water, and pH was adjusted to 5-6 with hydrochloric acid, and then EDC 1.7262 g and N-hydroxy succinimide was added to 2.87 g for 24 hours, and then 200 mg Pb-Cd was added. -NH2 room temperature reaction 24 h, centrifugal washed solid product, which is P...

Embodiment 3

[0101] A method of preparing a composite nano drug carrier system, including the steps of:

[0102] Synthetic PB-CD-NH 2

[0103] The PB 200 mg was scattered in PBS of 100 mL of pH of 5.5, and then the ice water bath was 4 ° C, EDC (1- (3-dimethylaminopropyl) -3-ethyl carbide hydrochloride 2.87 G and N-hydroxy succinimide 1.7262 g, reaction for 24 hours; then adding 180 mGEDA-β-CD and 20 mg of cystamine dihydrochloride for 24 hours, centrifugation, washed the resulting solid product, which is PB-CD-NH. 2 .

[0104] 2. Synthesis PB-C-DOTS-CD

[0105] The carbon quantum point 60 mg was scattered in PBS of 100 mL of a pH of 5.5, and then EDC1.7262G and N-hydroxy succinimide were added at 4 ° C for 24 hours, and then 200 mg Pb-CD-NH2 was added. The reaction was cleaned at room temperature for 24 h, and the resulting solid product was washed, which was PB-C-DOTS-CD.

[0106] 3. Synthesize PLL (NF)

[0107] It is weighed 200 mg PLL, 100 mg of potassium carbonate and 5-chloro-2-nithenyl...

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Abstract

The invention discloses a drug carrier system, which uses carbon quantum dots and Prussian blue modified by β-cyclodextrin as a substrate, and grafts polylysine with NO donor 5-chloro-2-nitrophenyltrifluoromethane After combination, it is modified with folic acid. The drug carrier system provided by the present invention can achieve effective enrichment at the tumor site through folic acid targeting and the EPR effect at the tumor site. Under near-infrared laser irradiation, the Prussian blue nanoparticles can ablate tumor cells through excellent photothermal conversion efficiency. At the same time, the drug carrier system can control the release of nitric oxide under the light of 400nm wavelength, thereby improving the EPR effect and enhancing the enrichment of nanoparticles in tumor sites. At the same time, NO can induce tumor cell apoptosis and reverse multidrug resistance. inhibit tumor growth.

Description

Technical field [0001] The present invention belongs to the field of pharmaceutical carrier, and more particularly to a pharmaceutical carrier system having a targeted administration capability in cancer cells and a preparation method thereof. Background technique [0002] In recent years, with the deterioration of the natural environment, the incidence of cancer is getting higher and higher. Traditional cancer treatment methods include: surgery, chemotherapy, and radiotherapy. Although it can extend the life of the patient to a certain extent, these treatment methods are generally more depolytesis, damage to normal tissue and large wounds, thereby limiting its treatment effect on tumors. Therefore, developing new treatment methods has received more and more attention. [0003] At present, chemotherapy is the main treatment plan for anti-tumor, but chemotherapy is challenged by multidrug resistance (MDR), which limits the efficacy of chemotherapy. It is often used to adopt high d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00A61K41/00A61K47/61A61K47/64A61K47/54A61P35/00
CPCA61K41/0042A61K41/0052A61K49/0067A61K47/545A61K47/61A61K47/645A61P35/00A61K2300/00
Inventor 李草陈重银万立辉陈辉段军林徐翔宇卢金博罗毕矗江兵兵
Owner HUBEI UNIV
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