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A method for rapid detection of mercury content in food based on ionic liquid

A technology of ionic liquid and food, applied in the field of analytical chemistry, can solve the problems of combined use, no sensor, low extraction efficiency, etc., and achieve the effect of less demand, simple operation and high selectivity

Active Publication Date: 2022-03-25
CAPITAL NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2008, Pronaiss researched a series of ionic liquids on the extraction ability of various metals. It was found that two ionic liquids [MPYRROS2] and [MPIPS2] containing two sulfide bonds have strong effects on mercury and copper ions. Extraction ability, the extraction efficiency of other cations is very low (Industrial&Engineering Chemistry Research, 2007,47(15):5080-5086.)
However, there is no report on the enrichment of mercury ions in food by ionic liquid, let alone any report on its use in combination with sensors

Method used

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  • A method for rapid detection of mercury content in food based on ionic liquid
  • A method for rapid detection of mercury content in food based on ionic liquid
  • A method for rapid detection of mercury content in food based on ionic liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1. Drawing of working curve.

[0032] Mercury solution, deoxyribozyme oligonucleotide EAD2 (5'-CTGGGAGGGAGGGAGGGA-3') solution, hemin solution, 2,2-azido-bis(3-ethyl) used in all the following examples - benzothiazole-6-sulfonic acid) diammonium salt (ABTS) and hydrogen peroxide solution, both in 4-hydroxyethylpiperazine ethanesulfonic acid buffer solution A (25mM HEPES, 20mM KNO 3 ,200mM NaNO 3 ,150mM NH 4 Cl, 0.025% (w / v) Triton X-100, and 1% (v / v) DMSO)) were dilution solvents.

[0033] like figure 1 As shown, 10 μL of ionic liquid (N-octylpyridine tetrafluoroborate) was added to 1 mL of mercury ion solutions of different concentrations (0, 0.5, 1, 2, 5, 7, 10, 50, 100 nM). Heated at 80 °C for 10 min, and then quenched in an ice bath for 10 min. After centrifugation at 8000 r / min for 10 min, the ionic liquid at the bottom of the centrifuge tube was taken out. Using buffer solution A as a dilution solvent, prepare 60 μL of 0.5 μM DNAzyme oligonucleotide ...

Embodiment 2

[0035] Example 2. Determination of selectivity:

[0036] Take 10 μL of N-octylpyridine tetrafluoroboric acid and add it to 1 mL of 100 nM solution of different metal ions (Hg 2+ , Cr 3+ , Ni 2 + , Ca 2+ , Pb 2+ , Cu 2+ , Fe 3+ , Fe 2+ , Cd 2+ , Zn 2+ , Mg 2+ )middle. Heated at 80°C for 10min and quenched in ice bath for 10min. Centrifuge at 8000 r / min for 10 min to separate and take out N-octylpyridine tetrafluoroboric acid at the bottom of the centrifuge tube. Prepare 60 μL of 0.5 μM DNAzyme oligonucleotide EAD2 solution, heat at 95°C for 6 minutes, and then slowly cool to 37°C. Hemin (15 μL, 20 μM) was added to the above EAD2 solution, and incubated at 25° C. for 90 min to form a DNAzyme complex. 2 μL of the ionic liquid obtained by centrifugation and 40 μL of DNAzyme complex were incubated at 25°C for 30 min. Centrifuge at 8000 r / min, take the upper 35 μL DNAzyme complex, add buffer solution A and dilute to 100 μL. Then 3.75 mM 10 μL of ABTS and 15 mM 5 μL o...

Embodiment 3

[0038] Example 3. Determination of the extraction efficiency of ionic liquids enriched with different concentrations of mercury ions from buffer solutions and 20% nitric acid:

[0039] 10 μL of N-octylpyridine tetrafluoroborate was added to 1 mL of water containing different concentrations of mercury ions or 20% nitric acid solution (0, 0.5, 1, 2, 5, 7, 10, 50, 100 nM). Heated at 80°C for 10min and quenched in ice bath for 10min. Centrifuge at 8000 r / min for 10 min to separate and take out N-octylpyridine tetrafluoroboric acid at the bottom of the centrifuge tube. The supernatant was collected, and the concentration of mercury ions in the supernatant was measured by atomic fluorescence spectrophotometer. Instrument conditions 270V, 25mA. Measure 15s, delay 0.5s. The carrier current is 5% HCl, and the reducing agent is 2% NaBH 4 , 0.5%NaOH, the extraction efficiency (1-(C 初始 *V 初始 -C 萃取后 *V 萃取后 ) / C 初始 *V 初始 )*100%.

[0040] The result is as Figure 4 As shown, N-oct...

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Abstract

The invention relates to a method for quickly detecting mercury ions in food. The method includes three steps: (1) digestion of the food sample to be tested; (2) extraction of mercury ions from the food nitrification solution with ionic liquid; The ribozyme reaction system is mixed, the absorption value of the ultraviolet-visible light at 418nm of the water phase is detected, and the concentration of mercury ions is determined according to the working curve. This method combines the high-efficiency and high-selectivity ion enrichment ability of ionic liquids with the catalytic ability of deoxyribozyme, and realizes the effective and rapid enrichment of mercury ions in food digestion liquid with super acidic and oxidative properties. The collected ionic liquid was directly used for the convenient, sensitive and specific colorimetric detection of DNAzyme-catalyzed mercury ions. The method is fast and simple, and can also be applied to the detection of mercury ions in complex media such as soil and haze particles, or extended to the detection of other heavy metal ions.

Description

technical field [0001] The invention relates to a method for rapidly detecting mercury ions in food by utilizing the high-efficiency and high-selectivity ion enrichment ability and deoxyribozyme catalytic ability of ionic liquids, and belongs to the technical field of analytical chemistry. Background technique [0002] Commonly used heavy metal detection methods include atomic emission spectrometry, atomic absorption spectrometry, atomic fluorescence spectrometry, mass spectrometry and so on. These traditional detection methods based on large-scale instruments have high requirements on the professionalism of operators, the equipment is large and expensive, and the pretreatment is complicated, which is not suitable for rapid on-site detection. The biosensor technology based on functional nucleic acid developed in recent years has the advantages of fast response, convenient operation, and no need for large-scale equipment. It has attracted much attention because of its good ap...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31G01N1/44G01N1/34G01N21/64
CPCG01N21/31G01N1/44G01N1/34G01N21/6404
Inventor 娄新徽黄旸薛颖王正
Owner CAPITAL NORMAL UNIVERSITY
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