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Enzyme substrate determination agent for coliform in water and preparation method thereof

A coliform and enzyme substrate technology, applied in the field of water pollution detection, can solve the problems of color reaction delay, low sensitivity of coliform, complex overall design, etc., achieve short validity period, easy transportation and storage, and improve detection sensitivity Effect

Pending Publication Date: 2019-08-20
奎泰斯特(上海)科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This measuring agent needs to strike a balance between the enzymatic reaction of galactosidase and glucuronidase. Its overall design is relatively complicated, and there are delays in the color reaction and low sensitivity when detecting coliform bacteria. question

Method used

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  • Enzyme substrate determination agent for coliform in water and preparation method thereof
  • Enzyme substrate determination agent for coliform in water and preparation method thereof
  • Enzyme substrate determination agent for coliform in water and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A coliform group enzyme substrate assay reagent in water, its raw material comprises the following components:

[0021] The sodium chloride of 8g, the o-nitrophenyl β-D-galactopyranoside of 0.3g, the 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid of 6.3g, the N-( 2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid, 0.5g of magnesium sulfate, 5g of ammonium sulfate, 0.1g of carbenicillin disodium, 0.02g of manganese sulfate, 0.5g of TBS buffer agent and 3g of soy lecithin.

[0022] Its preparation process is as follows:

[0023] S1: Heat the pH adjuster and indicator to 65°C until the moisture in the pH adjuster and indicator is less than 5% respectively;

[0024] S2: Grinding the pH regulator and the indicator obtained in S1 into powders, until the powder particles of the pH regulator and the indicator are respectively smaller than 40 meshes;

[0025] S3: Stir and mix the pH regulator and the indicator obtained in S2 to obtain a mixed powder of the pH regulator an...

Embodiment 2

[0032] A coliform group enzyme substrate assay reagent in water, its raw material comprises the following components:

[0033] The sodium chloride of 9g, the o-nitrophenyl β-D-galactopyranoside of 0.4g, the 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid of 6.4g, the N-( 2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid, 0.65g of magnesium sulfate, 5g of ammonium sulfate, 0.2g of carbenicillin disodium, 0.03g of manganese sulfate, 0.65g of TBS buffer agent and 3g of soy lecithin.

[0034] Its preparation process is with embodiment 1.

[0035] Get 1.5g of the finished product of Example 2 and drop it into a 100ml water sample for detection. At the same time, the two existing products of the American IDEXX colilert enzyme substrate reagent and the American Neogen colitag enzyme substrate reagent were used as comparison examples, and 1.5g of the finished product was put into 100ml of water samples for testing. The test results are shown in Table 2 below:

[0036]

[00...

Embodiment 3

[0040] A coliform group enzyme substrate assay reagent in water, its raw material includes components:

[0041] The sodium chloride of 10g, the o-nitrophenyl β-D-galactopyranoside of 0.5g, the 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid of 6.5g, the N-( 2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid, 0.8g of magnesium sulfate, 7g of ammonium sulfate, 0.3g of carbenicillin disodium, 0.04g of manganese sulfate, 0.8g of TBS buffer agent and 5g of soy lecithin.

[0042] Its preparation process is with embodiment 1, embodiment 2.

[0043] Get 1.5g of the finished product of Example 3 and drop it into a 100ml water sample for detection. At the same time, the two existing products of the American IDEXX colilert enzyme substrate reagent and the American Neogen colitag enzyme substrate reagent were used as comparison examples, and 1.5g of the finished product was put into 100ml of water samples for testing. The test results are shown in Table 3 below:

[0044]

[0045...

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Abstract

The invention discloses an enzyme substrate determination agent for coliform in water and a preparation method thereof. The enzyme substrate determination agent for coliform in water comprises the following components in parts by weight: 8 to 10 parts of a PH regulator; 9.4 to 10 parts of an indicator; 5.62 to 8.14 parts of an inhibitor; and 3.5 to 5.8 parts of a production agent. The production agent consists of 0.5 to 0.8 part of a TBS buffer and 3 to 5 parts of soybean lecithin. The determination agent can carry out microbial detection on coliform in water, improve detection efficiency andimprove detection sensitivity at the same time.

Description

technical field [0001] The invention belongs to the technical field of water pollution detection, and in particular relates to an enzyme-substrate assay agent for coliform bacteria in water and a preparation method for the enzyme-substrate assay agent for coliform bacteria in water. Background technique [0002] In the field of water pollution detection, the current microbial detection mainly includes three indicators: coliform, heat-resistant coliform, and E. coli. Among them, coliforms and heat-resistant coliforms are important indicators for judging that water bodies are subject to fecal-oral contamination of animal origin. Existing assay reagents can simultaneously detect coliforms and Escherichia coli in water, and its formula includes ammonium sulfate, manganese sulfate, zinc sulfate, magnesium sulfate, sodium chloride, calcium chloride, sodium sulfite, amphotericin B, orthonitrate phenyl β-D-galactopyranoside, 4-methylumbelliferone-β-D-glucuronide, nightshade plant e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/10C12R1/19
CPCC12Q1/10G01N2333/245
Inventor 赵雪涛
Owner 奎泰斯特(上海)科技有限公司
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