Glyceride mixture rich in polyunsaturated fatty acids and preparation method and applications thereof
A technology of unsaturated fatty acids and mixtures, which is applied in the field of glyceride mixtures rich in polyunsaturated fatty acids and its preparation and application, can solve the problems of unsatisfactory and unstable health care effects, achieve the effect of reducing blood fat and weight loss, reduce Blood pressure and blood lipid lowering effect
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Embodiment 1
[0022] Commercially available fish oil fatty acid, algal oil fatty acid, linolenic acid, conjugated linoleic acid are used as raw materials, mixed in a certain proportion (PUFA composition is CLA 10.85%, ALA 5.41%, GLA 3.22%, CNLA 1.04%, AA 10.28%, EPA30 .15%, DPA 3.14%, DHA 31.17%). Utilize partial glyceride lipase Lipase G50 (derived from penicilliumcamembertii, purchased from Amano Enzyme Co., Ltd., Japan) to catalyze the reaction of fatty acid and glycerol to synthesize a mixture containing diglyceride and monoglyceride, the amount of lipase added is 1%, and the amount of water added 0.5%. After 12 hours of esterification reaction, the mixture was centrifuged, and the upper oil phase was recovered, and the fatty acid in it was separated and removed by molecular distillation to obtain a product mainly composed of glyceride. The content of diglyceride in the product after molecular distillation is 51.5%, the content of monoglyceride is 46.0%, the content of triglyceride is ...
Embodiment 2
[0024] Commercially available fish oil fatty acid, conjugated linoleic acid, linolenic acid, and pomegranate seed oil fatty acid are used as raw materials and mixed in a certain proportion (PUFA composition is CLA 3.24%, ALA 6.01%, GLA 1.65%, CNLA 37.55%, AA 1.31%, EPA20.75%, DPA 2.42%, DHA 21.42%). A mixture containing diglyceride and monoglyceride was synthesized by using partial glyceride lipase Lipase G50 to catalyze the reaction of fatty acid and glycerol. The added amount of lipase was 1%, and the added amount of water was 0.5%. After 12 hours of esterification reaction, the mixture was centrifuged, and the upper oil phase was recovered, and the fatty acid in it was separated and removed by molecular distillation to obtain a product mainly composed of glyceride. The content of diglyceride in the product after molecular distillation is 54.3%, the content of monoglyceride is 42.7%, the content of triglyceride is 2.2%, and the content of free fatty acid is 0.7%. The total ...
Embodiment 3
[0026] Commercially available fish oil fatty acid, conjugated linoleic acid, linolenic acid, and pomegranate seed oil fatty acid are used as raw materials and mixed in a certain proportion (PUFA composition is CLA 3.58%, ALA 5.94%, GLA 1.58%, CNLA 37.16%, AA 1.27%, EPA20.53%, DPA 2.37%, DHA 21.83%). A mixture containing diglyceride and monoglyceride was synthesized by using partial glyceride lipase Lipase G50 to catalyze the reaction of fatty acid and glycerol. The added amount of lipase was 1%, and the added amount of water was 0.5%. After 12 hours of esterification reaction, the mixture was centrifuged, and the upper oil phase was recovered, and the fatty acid in it was separated and removed by molecular distillation to obtain a product mainly composed of glyceride. The content of diglyceride in the product after molecular distillation is 88.3%, the content of monoglyceride is 10.4%, the content of triglyceride is 1.2%, and the content of free fatty acid is 0.1%. The total ...
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