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Gas chromatography-tandem mass spectrometry analysis method for flavors and fragrances used for tobaccos

A gas chromatography and tandem mass spectrometry technology, which is applied to the detection field of flavor and fragrance components for tobacco, can solve the problem of limited number of flavor components, and achieve the effects of good precision and repeatability, high sensitivity and high accuracy

Active Publication Date: 2019-08-16
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Constrained by analysis method problems such as sample matrix interference and insufficient sensitivity of GC / MS, the number of aroma components covered by previous studies is relatively limited, only dozens of kinds can be covered

Method used

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  • Gas chromatography-tandem mass spectrometry analysis method for flavors and fragrances used for tobaccos
  • Gas chromatography-tandem mass spectrometry analysis method for flavors and fragrances used for tobaccos
  • Gas chromatography-tandem mass spectrometry analysis method for flavors and fragrances used for tobaccos

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0043] Weigh 2g of tobacco flavor and fragrance samples into a 50mL stoppered centrifuge tube, add 4mL of water, vortex for 2min; add 10mL of acetonitrile and 80μL of 30.0mg / L d 8 -Acetophenone internal standard working solution, vortex at 2500r / min for 2min, then add 2.5g sodium chloride, vortex at 2500r / min for 1min, centrifuge at 8000r / min for 3min; take 1mL supernatant in a 2mL centrifuge tube , add 150 mg of anhydrous magnesium sulfate and 5 mg of multi-walled carbon nanotubes, immediately vortex at 2500 r / min for 2 min, and centrifuge at 8000 r / min for 3 min; the supernatant is filtered through a 0.22 μm organic phase filter and analyzed by GC-MS / MS;

[0044] GC-MS / MS analysis conditions are as follows:

[0045] Gas chromatography conditions: Chromatographic column: elastic quartz capillary column, stationary phase is 50% phenyl-methylpolysiloxane, size 60m×0.25mm×0.25μm, pre-column connected in series at the inlet port (5m×0.25mm) ; Inlet temperature: 280°C; Injection ...

example 2

[0060] Weigh 2g of tobacco flavor and fragrance samples into a 50mL stoppered centrifuge tube, add the standard solution to make the addition level of the target substance 0.5μg / g, add 4mL of water, vortex for 2min; add 10mL of acetonitrile and 80μL of 30.0mg / L d 8 -Acetophenone internal standard working solution, vortex at 2500r / min for 2min, then add 2.5g sodium chloride, vortex at 2500r / min for 1min, centrifuge at 8000r / min for 3min; take 1mL supernatant in a 2mL centrifuge tube , add 150 mg of anhydrous magnesium sulfate and 5 mg of multi-walled carbon nanotubes, immediately vortex at 2500 r / min for 2 min, and centrifuge at 8000 r / min for 3 min; the supernatant is filtered through a 0.22 μm organic phase filter and analyzed by GC-MS / MS; Refer to Example 1 for GC-MS / MS analysis conditions.

[0061] According to the above operation, 5 parallel tests within a day and 5 parallel tests during the day were carried out. The measurement results were the intra-day precision and the...

example 3

[0069] The method of Example 1 was used to detect 5 different tobacco flavor and fragrance samples, and a total of 254 target objects were detected. Among them, a total of 162 target substances were detected in sample No. 1, and the highest content was 3-methyl-2,5-furandione, followed by furfural, 3-furfural, benzaldehyde and ethyl lactate; 189 target substances were detected, the highest content was methylcyclopentenolone, followed by eugenol, 3-methyl-2,5-furandione, ethyl lactate and solanone; total detection of No. 3 sample 176 target substances were detected, the highest content was ethyl lactate, followed by 3-methyl-2,5-furandione, solanone, ethyl nonanoate and α-angelica lactone; a total of 4 samples were detected 254 kinds of target substances, the highest content is solanone, followed by 3-methyl-2,5-furandione, macrostigmatrienone, ethyl palmitate and ethyl oleate; a total of 5 samples were detected Among the 253 targets, solanone had the highest content, followed...

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Abstract

The invention relates to a gas chromatography-tandem mass spectrometry analysis method for flavors and fragrances used for tobaccos, and belongs to the technical field of the ingredient detection of flavors and fragrances used for tobaccos. The method is characterized in that: after a sample of flavors and fragrances used for tobaccos is diluted by ultrapure water and is extracted by organic solvent, a multi-wall carbon nanotube is used as reverse solid phase dispersion adsorbent, the sample is purified by the vortex and centrifugation, and a gas chromatography-tandem mass spectrometry technology is combined for realizing the simultaneous detection of 345 fragrance ingredients in the flavors and fragrances used for tobaccos. The method has the advantages of being simple and quick in operation, high in flux, low in cost, small in solvent dosage, friendly to environment, wide in analyzable compound range, high in accuracy, good in precision, high in sensitivity, good in repeatability andthe like, and can meet a requirement on quickly analyzing and detecting the ingredients of the flavors and fragrances used for tobaccos.

Description

technical field [0001] The invention belongs to the technical field of detection of tobacco flavors and fragrances, in particular to a simultaneous determination of tobacco flavors and fragrances by ultrapure water dilution, organic solvent extraction, multi-walled carbon nanotube purification, and gas chromatography-tandem mass spectrometry (GC-MS / MS) The analysis method of 345 kinds of aroma components. Background technique [0002] Tobacco flavors and fragrances are essential substances in the production of cigarettes. They can not only endow cigarette smoke with a specific taste, make up for and enhance the aroma of cigarette smoke, but also make cigarette smoke soft and delicate. The core technology plays an important role in improving the taste of cigarettes and highlighting the style of cigarettes. Therefore, obtaining systematic and comprehensive analysis data of key sensory components of tobacco flavors and fragrances is the basis and basis for cigarette design, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/08
CPCG01N30/02G01N30/06G01N30/08G01N30/14G01N2030/045G01N2030/062G01N2030/085G01N2030/146
Inventor 陈黎赵嘉幸任宗灿刘惠民谢复炜崔华鹏王晓瑜刘绍锋樊美娟郭吉兆
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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