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Method for rapidly detecting 8 phenolic acids in liquor product by serial connection of ultra-performance convergence chromatography and QD

An ultra-efficient phase combination and phenolic acid technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of liquid chromatography, such as the consumption of organic reagents, trouble, and the small molecular weight of phenolic acids, to achieve excellent separation effect , Simple and fast operation, good repeatability

Inactive Publication Date: 2019-05-31
ANHUI GUJING DISTILLERY +1
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Problems solved by technology

Among them, liquid chromatography consumes a large amount of organic reagents, and the operation is time-consuming and troublesome, and the mobile phase of the reagents used is toxic, which is not conducive to human health.
It has been reported in the literature that the tandem quadrupole method was used to test 8 kinds of phenolic acids. However, due to the small molecular weight of phenolic acids, there are no fragments with high abundance, and the fragments are too small, and the detection results are easily interfered.
[0006] In addition, the content of phenolic acids in wine products is relatively small, and the original high-performance liquid chromatography method cannot meet the test requirements

Method used

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  • Method for rapidly detecting 8 phenolic acids in liquor product by serial connection of ultra-performance convergence chromatography and QD
  • Method for rapidly detecting 8 phenolic acids in liquor product by serial connection of ultra-performance convergence chromatography and QD
  • Method for rapidly detecting 8 phenolic acids in liquor product by serial connection of ultra-performance convergence chromatography and QD

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Embodiment Construction

[0051] The technical solutions of the present invention will be further described below in conjunction with specific embodiments.

[0052] The present embodiment measures 8 kinds of phenolic acids in a health wine according to the following steps:

[0053] The instruments used are: ultra-high performance convergence chromatograph (equipped with QDa detector); 3.0×100mm, 1.8μm HSS C18SB Column (3.0×100mm, 1.7μm); vortex instrument; pipette gun.

[0054] The specific steps are:

[0055] 1. Drawing of standard curves of 8 kinds of phenolic acids

[0056] Take gallic acid, chlorogenic acid, caffeic acid, ferulic acid, p-coumaric acid, luteolin, catechin and succinic acid, prepare 1-2 ng / mL stock solutions, and then dilute them in 5 different Mix the series of standard working solutions, then take 1.0-2.0mL of the mixed series of standard working solutions for injection, and use an ultra-high performance convergence chromatograph connected in series with a QDa mass spectrometer f...

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Abstract

The invention discloses a method for rapidly detecting 8 phenolic acids in a liquor product by serial connection of an ultra-performance convergence chromatography and a QD. After a liquor sample to be detected is extracted by methanol, a dedicated chromatographic column of the BSS C18 convergence is taken as a separation column, carbon dioxide (A)+methanol (B) are taken as a mobile phase for gradient elution, a sample is separated by the ultra-performance convergence chromatography (UPC2), and then a QDa mass spectrometry detector is employed for detection. The method is simple and rapid andconvenient, and accurate and reliable, and can be used for detection of the content of the 8 phenolic acids in liquors.

Description

technical field [0001] The invention relates to a method for quickly detecting 8 kinds of phenolic acids in wine products by connecting QDa in series with ultra-high-efficiency convergence chromatography, and belongs to the technical field of wine analysis. Background technique [0002] Liquor flavor is an important feature that determines its quality. There are many research reports on volatile aroma substances in liquor. For example, thousands of substances can be identified from Luzhou-flavor liquor by using the most advanced comprehensive two-dimensional gas phase time-of-flight mass spectrometer. However, liquor is the product of solid-state fermentation distillation, and this solid-state distillation method makes certain non-volatile substances exist in liquor. For example, the non-volatile substance lactic acid is an important component in liquor, which plays a very important role in the flavor formation and quality of liquor. [0003] According to literature reports...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/72
Inventor 王银辉刘国英李安军万春环何宏魁丁峰马金同
Owner ANHUI GUJING DISTILLERY
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