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Aspergillus aculeatus strain and application thereof

A technology of Aspergillus aculeatus and Aspergillus aculeatus, which is applied in application, fungicides, fungi and other directions, can solve problems such as pesticide residues, and achieve the effect of promoting growth

Active Publication Date: 2019-08-16
TIANJIN AGRICULTURE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to overcome problems such as cucumber wilt and other difficult-to-control diseases in production, and serious pesticide residues caused by the prevalence of diseases and the use of chemical pesticides, the invention provides a kind of Aspergillus aculeatus strain, and utilizes the Aspergillus aculeatus strain to promote The application technology of cucumber plant growth, prevention and treatment of cucumber wilt, and induction of plant acquired disease resistance. This technology can not only effectively promote the growth of cucumber plants and be used as a biological pesticide, but also has low cost, simple operation, no pollution, and does not pose a threat to the environment. harm

Method used

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  • Aspergillus aculeatus strain and application thereof
  • Aspergillus aculeatus strain and application thereof
  • Aspergillus aculeatus strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Isolation, purification and identification of strains

[0027] 1. Soil collection: In this experiment, the soil for growing grapes in Ninghe, Tianjin, China was collected. The collected soil samples were stored at 4°C for use in screening soil fungi.

[0028] 2. Separation and purification of soil fungi: (1) Add 5g of soil to 1% saline to form soil dilutions of 1:102, 1:103, 1:104, 1:105, and 1:106, and shake them enough Draw 100 μL of each of the above-mentioned soil dilutions with different sparseness and apply them on the PDA solid medium containing ampicillin, and then place the PDA solid medium at 28°C for 2 to 3 days. (2) After culturing, select typical fungal colonies on the PDA solid medium and continue pure culture to obtain Aspergillus aculeatus Chinese Common Microorganism Collection Management Center (CGMCC) No. 17191.

[0029] 3. Strain identification: (1) Extract fungal DNA by CTAB method. The genomic DNA of strain TJ was extracted using the fungal g...

Embodiment 2

[0030] Example 2: Experiment to promote the growth of cucumber plants

[0031] 1. Fermentation of strains

[0032] Medium: PDB medium (200g potato, 20g glucose, 1000mL water, natural pH)

[0033] Strain culture: Put a little fungus in the solid PDA medium into the liquid medium formula (200g potato, 20g glucose, 16g agar powder, 1000ml distilled water, sterilized at 121°C for 20min), put it in the shaker box and shake it for cultivation time 7-10d.

[0034] 2. Cucumber seedling irrigation

[0035] Take 20mL and 10mL of the shaken bacteria and pour them into a 500mL Erlenmeyer flask respectively, add distilled water to 400mL and 500mL, and dilute it to a constant volume of 20 times and 50 times of the same bacterial liquid with different concentrations, Distilled water was used as a control. Pour the diluted liquid evenly into the soil around the roots of the plants in the seedling tray, and pour 10 mL of each seedling plant.

[0036] 3. Determination of Cucumber Growth Index

[0037] Me...

Embodiment 3

[0044] Example 3: Experiment of inducing cucumber resistance to Fusarium wilt

[0045] 1. Fermentation of strains

[0046] Medium: PDB medium (200g potato, 20g glucose, 1000mL water, natural pH)

[0047] Strain culture: Put a little fungus in the solid PDA medium into the liquid medium formula (200g potato, 20g glucose, 16g agar powder, 1000ml distilled water, sterilized at 121°C for 20min), put it in the shaker box and shake it for cultivation time 7-10d.

[0048] 2. Cucumber seedling irrigation

[0049] Take 20mL and 10mL of the shaken bacteria and pour them into 500mL Erlenmeyer flasks, add distilled water to 400mL and 500mL, and dilute them to 20 times and 50 times of the same bacteria with different concentrations. The diluted liquid and the volume mixture of cucumber Fusarium wilt solution 2:1, 3:1, the cucumber seedlings in each pot are irrigated with 5mL roots, and 5ml of cucumber fusarium wilt solution is set to irrigate the roots. Observe the phenomenon in the clear water (C...

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Abstract

The invention discloses an aspergillus aculeatus strain and an application thereof. The aspergillus aculeatus strain NH9 is collected in a CGMCC (China General Microbiological Culture Collection Center) on April 1, 2019, and the collection number of the strain is CGMCC No. 17191. Cucumber plant growth can be effectively promoted, the strain can be used as a biological pesticide and is low in cost,simple to operate and free from pollution, and environmental harm is avoided.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a strain of Aspergillus aculeatus and its application. Background technique [0002] Aspergillus aculeatus is a kind of Aspergillus Niger, a filamentous fungus belonging to the genus Aspergillus. Aspergillus aculeatus has various biologically active metabolites, such as the antibacterial factor auleacinsA–G, the α-glucosidase inhibitor aspergillusolA, the antimicrobial factor secalonicacids D and F and so on. These compounds have antimicrobial, antifouling, antifeedant activity and phytotoxin activity. In the existing reports, the ability of such strains to induce disease resistance has not been explored in depth. [0003] Salicylic acid is an important signal molecule in plant resistance to various diseases and response to pathogenic attacks, and it plays a key role in regulating the physiological growth of plants. It is an important inducer of plant systemic acquired resista...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00A01P21/00C12R1/66
CPCC12R2001/66C12N1/145A01N63/30
Inventor 王远宏常若葵柴庆凯张斌
Owner TIANJIN AGRICULTURE COLLEGE
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