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Method of adopting candida olive to promote wound healing of postharvest potato tuber

A technology for olive candid silk and potato pieces, applied in the agricultural field, can solve problems such as pesticide residue environment and pollution, and achieve the effects of enhancing disease resistance, healthy and environmentally friendly postharvest callus, and promoting postharvest callus.

Active Publication Date: 2019-08-16
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for Candida olive to promote postharvest potato tuber callus to solve the problem that chemical drugs will cause pesticide residues and environmental pollution in promoting potato tuber callus

Method used

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  • Method of adopting candida olive to promote wound healing of postharvest potato tuber
  • Method of adopting candida olive to promote wound healing of postharvest potato tuber
  • Method of adopting candida olive to promote wound healing of postharvest potato tuber

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1. Potato tubers were processed according to the following steps:

[0026] A. Inoculate Candida olivaceus on the slope of the nutritional yeast glucose solid medium (NYDA) for the initial activation. The activation temperature is 28℃ and the activation time is 2 days. The activated Candida olivaceus is prepared with sterile water Yeast suspension;

[0027] B. Inoculate the yeast suspension prepared in step A into the nutrient yeast glucose liquid medium (NYDB), culture it for 24 hours at a temperature of 28°C and a rotation speed of 220r / min; re-inoculate the cultured yeast into the nutrition Yeast glucose liquid medium (NYDB) was continued to be re-cultured at a temperature of 28°C and a rotation speed of 220r / min to obtain the target yeast suspension, and the final concentration of yeast in the target yeast suspension was adjusted to 1×10 9 cells / mL;

[0028] C. Rinse the potato tubers with tap water first, then soak and disinfect with 1% sodium hypochlorite for 2 m...

Embodiment 2

[0041] Example 2. Potato tubers were processed according to the following steps:

[0042] A. Inoculate Candida olivaceus on the slant of the nutritional yeast glucose solid medium (NYDA) for initial activation. The activation temperature is 26℃ and the activation time is 3 days. The activated Candida olivaceus is prepared with sterile water Yeast suspension;

[0043] B. Inoculate the yeast suspension prepared in step A into the nutrient yeast glucose liquid medium (NYDB), culture it for 24 hours at a temperature of 28°C and a rotation speed of 220r / min; re-inoculate the cultured yeast into the nutrition Yeast glucose liquid medium (NYDB) was continued to be re-cultured at a temperature of 28°C and a rotation speed of 220r / min to obtain the target yeast suspension, and the final concentration of yeast in the target yeast suspension was adjusted to 1×10 8 cells / mL;

[0044] C. Rinse the potato tubers with tap water first, then soak and disinfect with 1% sodium hypochlorite for 2 minut...

Embodiment 3

[0046] Example 3. Potato tubers were processed according to the following steps:

[0047] A. Inoculate Candida olivaceus on the slope of the nutritional yeast glucose solid medium (NYDA) for the initial activation. The activation temperature is 30℃ and the activation time is 2 days. The activated Candida olivaceus is prepared with sterile water Yeast suspension;

[0048] B. Inoculate the yeast suspension prepared in step A into the nutrient yeast glucose liquid medium (NYDB), culture it for 24 hours at a temperature of 28°C and a rotation speed of 220r / min; re-inoculate the cultured yeast into the nutrition Yeast glucose liquid medium (NYDB) was continued to be re-cultured at a temperature of 28°C and a rotation speed of 220r / min to obtain the target yeast suspension, and the final concentration of yeast in the target yeast suspension was adjusted to 1×10 6 cells / mL;

[0049] C. Rinse the potato tubers with tap water first, then soak and disinfect with 1% sodium hypochlorite for 2 m...

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Abstract

The invention discloses a method of adopting candida oliva to promote wound healing of postharvest potato tuber, belongs to the field of agriculture and solves the problem that pesticide residue and environment pollution can be caused by adopting chemical drug to promote wound healing of potato tuber. The method includes following steps: inoculating candida oliva onto a bevel of a nutritional yeast glucose solid culture medium for activation, and using sterile water to prepare yeast suspension liquid; inoculating the yeast suspension liquid into a nutritional yeast glucose liquid culture medium for culture for two times; flushing harvested potato tuber, using sodium hypochloride for disinfection, using deionized water to flush, and drying; uniformly spraying target yeast suspension liquidonto the surface of a wound of the potato tuber, naturally air-drying, and putting the potato tuber into a punched preservation bag for wound healing. Pollution-free candida oliva is adopted to heal the wound of the postharvest potato tuber, has biocontrol effect on postharvest disease and pest and can enhance disease resistance of the potato tuber.

Description

Technical field [0001] The invention belongs to the field of agriculture, and specifically relates to a method for promoting the callus of post-harvest potato tubers by Candida olivaceus. Background technique [0002] Potato (Solanum tuberosum. L) is the fourth largest food crop in the world. It is widely cultivated all over the world and has a huge impact on world food security and economic development. Potato tubers will inevitably produce wounds during harvesting and transportation, and these wounds will become the main channels for harmful microorganisms to infect potato tubers. Potato tubers can form callus through self-callus, which provides the main protection to prevent the infection of pathogens. However, the natural callus of potato will be affected by the external environment. It is not suitable for callus temperature, humidity and oxygen. At high concentrations, potato callus will be further inhibited. Although some chemical drugs have been reported to promote the h...

Claims

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Application Information

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IPC IPC(8): A23B7/155A23B7/157
CPCA23B7/155A23B7/157
Inventor 郑晓渊毕阳姜红王斌王调兰
Owner GANSU AGRI UNIV
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