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Method for repairing oil-contaminated soil through reinforcement of biochar-base complex microbial inoculant

A compound bacterial agent, oil pollution technology, applied in the restoration of polluted soil and other directions, can solve the problems of slow growth of oil-degrading microorganisms, reduced survival rate, resource consumption, etc., and achieves easy control of process parameters, guaranteed degradation efficiency, and degradation quality. Guaranteed effect

Inactive Publication Date: 2019-08-13
YONKER ENVIRONMENTAL PROTECTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of degrading petroleum-contaminated soil or oil sludge in this method, free bacterial liquid is used as bacterial agent. Since free bacterial agent is easily affected by the environment in the polluted medium, the growth rate is slow, the survival rate is reduced, and the oil degradation performance is also easily affected. Therefore, it is necessary to add a large amount of biomass materials and crude surfactants as a means to enhance the ability to degrade petroleum, which not only consumes a lot of resources, but also excessive surfactants will slow down the growth of microorganisms degrading petroleum and reduce the degradation ability.

Method used

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  • Method for repairing oil-contaminated soil through reinforcement of biochar-base complex microbial inoculant
  • Method for repairing oil-contaminated soil through reinforcement of biochar-base complex microbial inoculant
  • Method for repairing oil-contaminated soil through reinforcement of biochar-base complex microbial inoculant

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preparation example Construction

[0033] (1) Preparation of biochar-based composite bacterial agent: using biochar as a carrier, mycobacterium CSC-6 bacterial liquid was prepared by adsorption method; the preservation number of mycobacterium CSC-6 is CCTCC No: M 2017726, a typical culture in China Preserved by the preservation center, the preservation date is November 27, 2017; biochar can be one or a mixture of rice husks, cotton stalks, japonica rice or rattan.

[0034] (2) Preparation of biosurfactant: with mannitol as carbon source, Pseudomonas aeruginosa W-3 is fermented and cultivated to obtain a fermentation broth containing biosurfactant, which is rhamnolipid ; The preservation number of Pseudomonas aeruginosa W-3 is CCTCC NO:M2010048, which was preserved in China Center for Type Culture Collection, and the preservation date was March 05, 2010.

[0035] (3) Conservation of polluted soil: the polluted soil is crushed, and the polluted soil is sieved with a fineness greater than 10 mesh sampling sieves, ...

Embodiment 1

[0041] In this embodiment, the method of strengthening and repairing oil-contaminated soil with a biochar-based composite bacterial agent is used to treat fresh oil-contaminated soil. The test method is as follows:

[0042] (1) Collect unpolluted fresh soil, pass through a 10-mesh sieve after crushing, add petroleum to make the oil content be 30000mg / kg, and obtain the fresh petroleum-contaminated soil that the oil content is 30000mg / kg;

[0043] (2) Using biochar as a carrier, prepare mycobacterium CSC-6 bacterium liquid by adsorption method, wherein the mass fraction of mycobacterium CSC-6 is 1%, and biochar is selected from rice husk charcoal; with mannitol as carbon source, the Pseudomonas aeruginosa W-3 is fermented and cultured to obtain a fermentation liquid containing a biosurfactant, and diluted with water to obtain a dilution of a fermentation liquid containing a biosurfactant, wherein the biosurfactant is rhamnolipid, and fermented with a biosurfactant The mass frac...

Embodiment 2

[0048] In this embodiment, the method of strengthening and repairing oil-contaminated soil with a biochar-based composite bacterial agent is used to treat fresh oil-contaminated soil. The test method is as follows:

[0049] (1) Collect unpolluted fresh soil, pass through a 10-mesh sieve after crushing, add petroleum to make the oil content be 50000mg / kg, and obtain the fresh petroleum-contaminated soil that the oil content is 50000mg / kg;

[0050] (2) Using biochar as a carrier, prepare mycobacterium CSC-6 bacterium liquid by adsorption method, wherein the mass fraction of mycobacterium CSC-6 is 1%, and biochar is selected from rice husk charcoal; with mannitol as carbon source, the Pseudomonas aeruginosa W-3 is fermented and cultured to obtain a fermentation liquid containing a biosurfactant, and diluted with water to obtain a dilution of a fermentation liquid containing a biosurfactant, wherein the biosurfactant is rhamnolipid, and fermented with a biosurfactant The mass frac...

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Abstract

The invention relates to a method for repairing oil-contaminated soil through reinforcement of a biochar-base complex microbial inoculant. The method includes the following steps of preparing a mycobacterium CSC-6 bacteria solution through an adsorption method with biochar as the carrier; conducting fermentation culture on pseudomonas aeruginosa W-3 with mannitol as the carbon source to obtain a fermentation liquid with a biosurfactant; curing contaminated soil, wherein after the contaminated soil is broken and sieved, a compound fertilizer is added, a diluent containing the fermentation liquid with the biosurfactant is added, the water content of the soil is adjusted to be 20-50%, a porous membrane is applied, and curing is conducted for 1-2 weeks at 20-30 DEG C; degrading the contaminated soil, wherein the biochar-base complex microbial inoculant is added to the cured contaminated soil, plowing is conducted once every 1-2 weeks, and the water content of the soil is adjusted to be 20-50%. Compared with the prior art, the method has the advantages of being high in petroleum degradation efficiency, high in degradation speed, simple in process, low in cost and the like.

Description

technical field [0001] The invention relates to a treatment method for oil sludge and oil-polluted soil, in particular to a method for strengthening and repairing oil-polluted soil with a biochar-based composite bacterial agent. Background technique [0002] Oil exploration and refining industries produce a large amount of oily sludge. Due to historical reasons, there is a lack of a sound regulatory system and treatment technology. Oil sludge is usually disposed of in landfills and piles without treatment, leaving serious sludge pollution problem. If oily sludge is not disposed of in a timely and appropriate manner, it will have varying degrees of impact on the surrounding environment. For example, the volatilization of oil and gas in oily sludge will pollute the air and cause great harm to the health of surrounding residents; the piled oily sludge will pollute surface water and even groundwater, which has potential health risks to humans; and a large amount of oily sludge ...

Claims

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Application Information

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IPC IPC(8): B09C1/10B09C1/00
CPCB09C1/00B09C1/10
Inventor 闫淑兰罗启仕崔长征任静赵秀红李梓
Owner YONKER ENVIRONMENTAL PROTECTION
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