Bifidobacterium pseudocatenulatum CCFM1047, composition, fermented food, application and bacterial agent of bifidobacterium pseudocatenulatum CCFM1047 and preparation method of bacterial agent of bifidobacterium pseudocatenulatum CCFM1047
A bifidobacterium and fermented food technology, applied in the field of microorganisms, can solve the problems of stimulating the gastrointestinal tract, liver function damage, affecting patients, etc., and achieve the effect of alleviating weight gain, alleviating metabolic syndrome, and improving content increase
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Embodiment 1
[0084] Example 1: Pseudo-small chain Bifidobacterium CCFM1047 has good tolerance to simulated gastrointestinal fluid
[0085] Inoculate the cryopreserved Bifidobacterium CCFM1047 in mMRS medium (MRS medium + 0.05% cysteine hydrochloride), culture anaerobically at 37°C for 48 hours, and then subculture in mMRS medium for 2 After ~3 times, take 1mL of the culture solution of Bifidobacterium pseudospermia CCFM1047, mix it with 9.0mL of pH 2.5 artificial simulated gastric juice (mMRS medium containing 1% pepsin, pH=2.5), and anaerobically For culture, samples were taken at 0h, 0.5h, 1h and 2h respectively, poured and cultured with mMRS agar medium to count plate colonies, determine the number of viable bacteria and calculate their survival rate.
[0086] The survival rate is the ratio of the logarithmic value of the number of viable bacteria at the time of sampling to the logarithmic value of the number of viable bacteria at the 0th hour in the culture solution, expressed in %. ...
Embodiment 2
[0091] Example 2: Pseudomonas Bifidobacterium CCFM1047 has no toxic side effects on C57BL / 6J mice
[0092] Pseudomonas bifidobacterium CCFM1047 cell resuspended in 3% sucrose solution to make a concentration of 3.0 × 10 8 CFU / mL bacterial suspension. Six healthy male C57BL / 6J mice with a body weight of about 20 g were taken. After one week of adaptation to the environment, the bacterial suspension of this concentration was administered orally once a day, observed for one week, and the death and body weight were recorded.
[0093] The results of these tests are listed in Table 3. These results indicate that feeding concentrations of 3.0×10 8 The CFU / mL pseudosmall chain Bifidobacterium CCFM1047 had no significant impact on mice, no significant change in body weight, and no death. The appearance of the mice had no obvious pathological symptoms.
[0094] Table 3 Changes in body weight and death of mice
[0095]
[0096] Note: -: no death of mice
Embodiment 3
[0097] Example 3: Pseudomonas bifidobacterium CCFM1047 has a recovery effect on the intestinal flora imbalance caused by high-fat feed
[0098] 36 healthy male C57BL / 6J mice weighing 18-20 g were taken, adapted to the environment for 1 week, and randomly divided into 6 groups: blank control group (NC), high-fat model control group (HFD), simvastatin control group (SC ), rosiglitazone control group (RH), Bifidobacterium pseudospermia CCFM1047 intervention group (CCFM1047), Bifidobacterium animalis BB12 control group (BB12), each group contained 6 mice, and the bacteria suspension The dose is 3.0 x 10 8 CFU / mL, resuspended in 3% sucrose solution. The grouping and treatment methods of experimental animals are shown in Table 4:
[0099] Table 4 Grouping of experimental animals
[0100]
[0101] At the end of the experiment, the fresh feces of the mice were collected and frozen at -80°C, the metagenome in the feces was extracted, and the structure of the intestinal flora was ...
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