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Cryopreservation resuscitation solution system capable of preserving ovary activity

A solution and system technology, applied in the preservation, application, animal husbandry, etc. of human or animal body, can solve the problem of not maintaining biological activity, affecting the quantity and quality of oocytes, and achieve the guarantee of quantity and quality, and the preservation time Long, easy-to-clean effect

Active Publication Date: 2019-08-02
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to overcome the shortcoming of prior art (that is, existing cryopreservation protection solution can not keep the biological activity after long-term cryopreservation of ovary, affects the problem of oocyte quantity and quality), provides the cryopreservation that preserves ovarian activity The resuscitation solution system provides the greatest microenvironment guarantee for maintaining the biological activity of ovaries after long-term cryopreservation, and at the same time meets the requirements of an ideal cryopreservation and resuscitation solution system for preserving ovarian activity. Convenience and economy lay the foundation

Method used

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  • Cryopreservation resuscitation solution system capable of preserving ovary activity
  • Cryopreservation resuscitation solution system capable of preserving ovary activity
  • Cryopreservation resuscitation solution system capable of preserving ovary activity

Examples

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Embodiment 1

[0024] Example 1 Preparation of cryopreservation and resuscitation solution system for preservation of ovarian activity

[0025] According to the component ratios in Table 1, a cryopreservation resuscitation solution system for preserving ovarian activity was prepared.

[0026] The preparation method of the cryopreservation and resuscitation solution system for preserving ovarian activity is as follows:

[0027] (1) Weigh a specified amount of gelatin, place it in a suitable container, add 2 times of gelatin mass to soak in water, fully swell and heat in a 100°C water bath to dissolve, add glycerin after fully dissolving, stir evenly, and reduce the temperature to 15 ℃, to form an aqueous solution of glycerol-gelatin complex;

[0028] (2) Dissolve heparin poloxamer in 4 times the mass of water, add melanoside, growth factor and palmitoyl tripeptide-1, stir evenly, add glycerol-gelatin complex aqueous solution, add vitamin C after mixing, and mix The solution system of cryopr...

Embodiment 2

[0034] Example 2 Evaluation of cryopreservation activity of sheep ovary

[0035] The solutions of each experimental group and control group prepared in Example 1 were subjected to the following cryopreservation and resuscitation process, and then the sheep ovary activity after resuscitation was measured, and the application effect of the cryopreservation and resuscitation solution system for preserving ovarian activity was evaluated.

[0036] Cryopreservation: Put the isolated and processed sheep ovaries into 50mL freezing bags containing the solution to promote the recovery of cryopreserved ovaries, and programmatically freeze them to -90°C for 5 minutes, and then put the cryopreservation bags containing the ovaries into the solution. Stored in nitrogen.

[0037] Resuscitation: One week later, the samples in each group were taken out from liquid nitrogen, placed in a 37°C water bath and thawed to a mixture of ice and water, and shaken constantly until completely melted.

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Abstract

The invention relates to a cryopreservation resuscitation solution system capable of preserving ovary activity. A glycerin and gelatin compound water solution with the concentration of 10-100 g / L is taken as a basic solution, the mass ratio of glycerin to gelatin in the glycerin and gelatin compound is 10:1, didymin, a growth factor, palmitoyl tripeptide-1, heparin poloxamer and vitamin C are added to the basic solution, an ovary is put in the solution system for cryopreservation, and the biological activity after cryopreserved ovary is resuscitated can be improved.

Description

technical field [0001] The present invention relates to a method for recovering the activity of cryopreserved ovaries, in particular to a cryopreservation solution for ovaries and a method for using the same. Background technique [0002] Ovaries are the reproductive organs of female animals. The ovarian microenvironment refers to the development and maturity of the ovary, whether the tissue and cell structure of the ovary is complete, the number of primordial follicles contained in the ovary, whether the primordial follicles can mature and ovulate, the thickness of the ovarian serosa, and whether the mature eggs can be successfully discharged. , pelvic inflammation and adhesion, ovarian hormone secreting cell function, the level and proportion of sex hormones in the body, peripheral nerve function and regulation, microvascular system function and blood supply (supply of oxygen, nutrients, exclusion of cell metabolites), etc. These factors together constitute the ovarian mi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 赵应征鲁翠涛姚情徐荷林习海涛诸葛德力
Owner WENZHOU MEDICAL UNIV
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