Preparation of Composite Glass Nanopores and Its Application to Biomolecular Detection

A technology of nano-channels and composite glass, which is applied in nano-medicine, nano-technology, nano-technology, etc., can solve the problems of high cost, complex detection instruments, and unfavorable popularization. Effect

Active Publication Date: 2021-05-25
WENZHOU UNIVERSITY
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Problems solved by technology

The detection methods at the present stage are all for the detection of a single biomolecule, and all of them require the use of expensive reagent materials such as fluorescent markers or biological kits. The cost is high and the required detection instruments are complicated, which is not conducive to popularization

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  • Preparation of Composite Glass Nanopores and Its Application to Biomolecular Detection
  • Preparation of Composite Glass Nanopores and Its Application to Biomolecular Detection
  • Preparation of Composite Glass Nanopores and Its Application to Biomolecular Detection

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Embodiment Construction

[0036] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings.

[0037] Step 1: Prepare glass nanopores; the operation method is as follows:

[0038] ①Take a glass tube whose specification is outer diameter: 1.2mm; inner diameter: 0.6mm; length: 10mm;

[0039] ② Place the glass tube in concentrated sulfuric acid (H 2 SO 4 ), hydrogen peroxide (H 2 o 2 ) mixed solution, ultrasonic for about 15min. Rinse with high-purity water and ethanol, and then place it in high-purity water for later use;

[0040]③Clamp out the cleaned glass tube with tweezers, rinse it with ethanol, and dry it with nitrogen, and set it aside;

[0041] ④Then draw the glass tube into a glass nanochannel with a suitable pore size on a glass tube drawing machine with certain parameters (the parameters are shown in Table 1 above). The nanopore size obtained...

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Abstract

The invention discloses a method for preparing a composite glass nano-channel and its application in biomolecular detection. The specific operation steps are as follows: step 1: preparing a glass nano-channel; step 2: modifying the glass nano-channel prepared in step 1 Phospholipids; Step 3: Prepare composite glass nanopores; Step 4: Measure and record the current data between the electrodes of the phospholipids inserted with SWCNTs on the glass nanopores modified by picoammeter and patch clamp; The present invention utilizes a glass drawing instrument The glass nanopore channel is drawn, and then the phospholipid inserted with the single-walled carbon nanotube is modified in the glass nanopore channel through the capillary phenomenon of the nanopore. There is no need to ultrasonically cut long-time purified SWCNTs, nor to use microinjection probes to insert SWCNTs into the phospholipid bilayer, and the method is simple. The current signal change of different biomacromolecules can be measured through the specific pores of the composite glass nanopore, which is suitable for detecting other different target molecules, has universal applicability, and is conducive to popularization and use.

Description

technical field [0001] The invention relates to the field of nano-channel detection, in particular to a preparation method of a glass nano-channel composite glass nano-channel based on single-armed carbon nanotube (SWCNT) and phospholipid self-assembly and its application in biomolecular detection. Background technique [0002] As a new type of analysis and detection method, nanopore technology is widely used in nucleic acid sequencing, protein / peptide analysis, and detection of biological macromolecules and metal ions such as viruses and microorganisms. As people pay more and more attention to issues such as public safety and food and drug safety, higher requirements are put forward for the detection of toxic substances. In view of the advantages of high sensitivity and high selectivity of the nanopore analysis method, many research teams have applied it to the detection of toxic substances and conducted a lot of research work. The current detection methods are all for the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/28G01N33/487B82Y40/00B82Y5/00
CPCB82Y5/00B82Y40/00G01N27/28G01N33/48721
Inventor 刘楠楠叶婷艳唐星星董幼青
Owner WENZHOU UNIVERSITY
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