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Vaccine composition and preparation method thereof

A vaccine composition and the technology of the composition are applied in the direction of biochemical equipment and methods, vaccines, and veterinary vaccines, which can solve problems such as adverse effects, antigen interference effects, and loss of efficacy, and achieve small side effects, simplified immunization procedures, and vaccination. The effect of fewer seedlings

Active Publication Date: 2019-07-19
杭州牧达智能科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the biggest problem that needs to be solved for combination vaccines is the recent recognition that one vaccine in combination vaccines may have an adverse effect on the other vaccine, that is, "antigen interference effect"
Relevant technical personnel have found that when two existing vaccines are simply mixed, one of the vaccines or both vaccines in the combination vaccine may lose efficacy (see patent application CN101035559A)

Method used

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  • Vaccine composition and preparation method thereof
  • Vaccine composition and preparation method thereof
  • Vaccine composition and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Preparation and testing of PCV2-PRV-PPV-PRRSV quadruple inactivated vaccine composition

[0044] 1. Collect antigens

[0045] 1.1 Preparation of poisonous seeds for production

[0046] 1.1.1 Preparation of PCV2 SH strain:

[0047] Properly dilute the virus seed of PCV2 SH strain with virus diluent (i.e., serum-free MEM cell maintenance solution), inoculate it in a monolayer PK-15 cell culture at 0.01 MOI (multiplicity of infection), absorb at 37°C for 30 minutes, add 4% (v / v) Calf serum and 2mmol / L D-glucosamine hydrochloride MEM cell maintenance solution, cultured at 37°C for 4 days, frozen 2-3 times, harvested SH strain virus liquid, stored below -20°C, should be Not more than 2 months.

[0048] 1.1.2 Preparation of Bartha-K61 strain:

[0049] Properly dilute the Bartha-K61 strain virus seed with the virus diluent (i.e., serum-free MEM cell maintenance solution), inoculate it in a monolayer PK-15 cell culture at 0.01 MOI (multiplicity of infection), absorb at 37°C f...

Embodiment 2- Embodiment 5 and comparative example 1

[0097] Embodiment 2-Example 5 and Comparative Example 1 are all based on the method of Example 1, and the components and contents of the vaccine composition are adjusted. The specific adjustments and the components and contents of Example 1 are shown in Table 2 below.

[0098] The components and content of the vaccine composition of table two embodiment 1-embodiment 5 and comparative example 1

[0099]

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PUM

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Abstract

The invention belongs to the technical field of animal vaccines, and particularly discloses a vaccine composition. The vaccine composition comprises the following components in parts by weight of 20-25 parts of PCV2 antigen, 20-25 parts of PRV antigen, 20-25 parts of PPV antigen, 20-25 parts of PRRSV antigen, 0.05-0.1 part of bovine serum albumin, 0.5-1 part of polyvinylpyrrolidone, 0.2-0.5 part of glycine, 0.2-0.5 part of L-sodium glutamate and 0.1-0.2 part of cane sugar. The vaccine composition disclosed by the invention is prepared through the steps of collecting the antigen, performing freeze-drying on a composition stock solution and performing freeze-drying on the composition stock solution. Effective efficacy of four vaccines of PCV2, PRV, PPV and PRRSV is guaranteed, diseases caused by the PCV2, the PRV, the PPV and the PRRSV can be better prevented, and besides, mass production of the vaccine composition can be convenient.

Description

technical field [0001] The invention belongs to the technical field of animal vaccines, in particular to a vaccine composition and a preparation method thereof. Background technique [0002] Porcine circovirus disease, caused by porcine circovirus, is a disease with multiple clinical symptoms, such as anorexia, dyspnea, or slow movement. Porcine circovirus (PCV) is the smallest animal virus ever discovered. PCV is known to have two serotypes, PCV1 and PCV2. Among them, PCV2 is a pathogenic virus, which is the main pathogen of multisystemic wasting syndrome (PMWS) in weaned piglets. Pigs are highly susceptible to PCV2. Infected pigs can excrete the virus from nasal fluid, feces and other wastes, and infect pigs of different ages through oral and respiratory routes. After pregnant sows are infected with PCV2, they can be transmitted vertically through the placenta to infect piglets. [0003] Many studies have shown that PCV2 infection alone does not cause typical symptoms ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/12A61K39/245A61K39/23A61K39/39A61K9/19A61K47/42A61K47/32A61K47/18A61K47/26A61P31/20A61P31/22A61P31/14
CPCA61K39/12A61K39/39A61K9/19A61K47/42A61K47/32A61K47/183A61K47/26A61P31/20A61P31/22A61P31/14A61K2039/552A61K2039/70A61K2039/5252A61K2039/5254A61K2039/55588C12N2750/10034C12N2710/16734C12N2750/14334C12N2770/10034
Inventor 李遥杨彩娟易太江张丰昌陈晓威吴程雨
Owner 杭州牧达智能科技有限公司
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