Application of aspergillus terreus secondary metabolite-butyrolactone in preparation of medicament for treating diabetes
A technology for secondary metabolites and diabetes drugs, applied in the field of microbial drugs, can solve the problems of high difficulty in collecting marine samples and incomplete expression of metabolites, and achieve the effect of avoiding blood sugar levels, alleviating type 2 diabetes, and reasonable design.
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Embodiment 1
[0045] The preparation method of butyrolactone I, butyrolactone II and butyrolactone IV:
[0046] 1) Fungal material: The fungal strain Aspergillus terreus OUCMDZ-2739 was isolated from Enteromorpha samples from Shilaoren Beach in Qingdao, and the Enteromorpha samples were washed with sterile seawater, 75% ethanol and sterile water in sequence. Then, the samples were crushed with a mortar and pestle, and then stored in PDA medium (containing 200 g of potato extract, 20 g of glucose, 15 g of agar, and 1 L of seawater per liter) containing 100 mg / mL chloramphenicol at 28 ° C. After cultured until a single strain appeared, the strain was transferred to another PDA medium and stored at 4°C. Through multiphase taxonomic studies, colony morphology and 18S rRNA sequence analysis, and establishment of strain development trees, it was identified as soil Aspergillus OUCMDZ-2739;
[0047] 2) Fermentation: Aspergillus terreus OUCMDZ-2739 inoculated in the fermentation broth was fermented...
Embodiment 2
[0051] The preparation method of butyrolactone I, butyrolactone II and butyrolactone IV:
[0052] 1) Fungal material: The fungal strain Aspergillus terreus OUCMDZ-2739 was isolated from Enteromorpha samples from Shilaoren Beach in Qingdao, and the Enteromorpha samples were washed with sterile seawater, 75% ethanol and sterile water in sequence. Then, the samples were crushed with a mortar and pestle, and then stored in PDA medium (containing 200 g of potato extract, 20 g of glucose, 15 g of agar, and 1 L of seawater per liter) containing 100 mg / mL chloramphenicol at 28 ° C. After cultured until a single strain appeared, the strain was transferred to another PDA medium and stored at 4°C. Through multiphase taxonomic studies, colony morphology and 18S rRNA sequence analysis, and establishment of strain development trees, it was identified as soil Aspergillus OUCMDZ-2739;
[0053] 2) Fermentation: Aspergillus terreus OUCMDZ-2739 inoculated in the fermentation broth was fermented...
Embodiment 3
[0057] The preparation method of butyrolactone I, butyrolactone II and butyrolactone IV:
[0058] 1) Fungal material: The fungal strain Aspergillus terreus OUCMDZ-2739 was isolated from Enteromorpha samples from Shilaoren Beach in Qingdao, and the Enteromorpha samples were washed with sterile seawater, 75% ethanol and sterile water in sequence. Then, the samples were crushed with a mortar and pestle, and then stored in PDA medium (containing 200 g of potato extract, 20 g of glucose, 15 g of agar, and 1 L of seawater per liter) containing 100 mg / mL chloramphenicol at 28 ° C. After cultured until a single strain appeared, the strain was transferred to another PDA medium and stored at 4°C. Through multiphase taxonomic studies, colony morphology and 18S rRNA sequence analysis, and establishment of strain development trees, it was identified as soil Aspergillus OUCMDZ-2739;
[0059] 2) Fermentation: Aspergillus terreus OUCMDZ-2739 inoculated in the fermentation broth was fermented...
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