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Application of aspergillus terreus secondary metabolite-butyrolactone in preparation of medicament for treating diabetes

A technology for secondary metabolites and diabetes drugs, applied in the field of microbial drugs, can solve the problems of high difficulty in collecting marine samples and incomplete expression of metabolites, and achieve the effect of avoiding blood sugar levels, alleviating type 2 diabetes, and reasonable design.

Inactive Publication Date: 2019-07-12
嘉兴市爵拓科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the collection of marine samples is difficult, and only less than 5% of marine microorganisms have been isolated under routine laboratory conditions, and even the obtained microorganisms cannot fully express all their biosynthetic pathways in the marine environment metabolite

Method used

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  • Application of aspergillus terreus secondary metabolite-butyrolactone in preparation of medicament for treating diabetes
  • Application of aspergillus terreus secondary metabolite-butyrolactone in preparation of medicament for treating diabetes
  • Application of aspergillus terreus secondary metabolite-butyrolactone in preparation of medicament for treating diabetes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The preparation method of butyrolactone I, butyrolactone II and butyrolactone IV:

[0046] 1) Fungal material: The fungal strain Aspergillus terreus OUCMDZ-2739 was isolated from Enteromorpha samples from Shilaoren Beach in Qingdao, and the Enteromorpha samples were washed with sterile seawater, 75% ethanol and sterile water in sequence. Then, the samples were crushed with a mortar and pestle, and then stored in PDA medium (containing 200 g of potato extract, 20 g of glucose, 15 g of agar, and 1 L of seawater per liter) containing 100 mg / mL chloramphenicol at 28 ° C. After cultured until a single strain appeared, the strain was transferred to another PDA medium and stored at 4°C. Through multiphase taxonomic studies, colony morphology and 18S rRNA sequence analysis, and establishment of strain development trees, it was identified as soil Aspergillus OUCMDZ-2739;

[0047] 2) Fermentation: Aspergillus terreus OUCMDZ-2739 inoculated in the fermentation broth was fermented...

Embodiment 2

[0051] The preparation method of butyrolactone I, butyrolactone II and butyrolactone IV:

[0052] 1) Fungal material: The fungal strain Aspergillus terreus OUCMDZ-2739 was isolated from Enteromorpha samples from Shilaoren Beach in Qingdao, and the Enteromorpha samples were washed with sterile seawater, 75% ethanol and sterile water in sequence. Then, the samples were crushed with a mortar and pestle, and then stored in PDA medium (containing 200 g of potato extract, 20 g of glucose, 15 g of agar, and 1 L of seawater per liter) containing 100 mg / mL chloramphenicol at 28 ° C. After cultured until a single strain appeared, the strain was transferred to another PDA medium and stored at 4°C. Through multiphase taxonomic studies, colony morphology and 18S rRNA sequence analysis, and establishment of strain development trees, it was identified as soil Aspergillus OUCMDZ-2739;

[0053] 2) Fermentation: Aspergillus terreus OUCMDZ-2739 inoculated in the fermentation broth was fermented...

Embodiment 3

[0057] The preparation method of butyrolactone I, butyrolactone II and butyrolactone IV:

[0058] 1) Fungal material: The fungal strain Aspergillus terreus OUCMDZ-2739 was isolated from Enteromorpha samples from Shilaoren Beach in Qingdao, and the Enteromorpha samples were washed with sterile seawater, 75% ethanol and sterile water in sequence. Then, the samples were crushed with a mortar and pestle, and then stored in PDA medium (containing 200 g of potato extract, 20 g of glucose, 15 g of agar, and 1 L of seawater per liter) containing 100 mg / mL chloramphenicol at 28 ° C. After cultured until a single strain appeared, the strain was transferred to another PDA medium and stored at 4°C. Through multiphase taxonomic studies, colony morphology and 18S rRNA sequence analysis, and establishment of strain development trees, it was identified as soil Aspergillus OUCMDZ-2739;

[0059] 2) Fermentation: Aspergillus terreus OUCMDZ-2739 inoculated in the fermentation broth was fermented...

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Abstract

The invention provides an application of aspergillus terreus secondary metabolite-butyrolactone in preparation of a medicament for treating diabetes, and belongs to the technical field of microbial medicaments. The aspergillus terreus secondary metabolite-butyrolactone comprises butyrolactone I, wherein the butyrolactone I is extracted according to the following method: fermenting aspergillus terreus OUCMDZ-2739 inoculated in fermentation broth for 25-35 days at 20-30 DEG C; filtering the fermentation broth after fermentation with denim to separate filtrate and mycelium, respectively extracting with ethyl acetate, mixing the obtained ethyl acetate extracts, and concentrating to obtain ethyl acetate solution extract; and further purifying the ethyl acetate solution extract to obtain the butyrolactone I. By the adoption of the application of the aspergillus terreus secondary metabolite-butyrolactone in the preparation of the medicament for treating diabetes, the medicament can inhibit activity of alpha-glucosidase, regulate a composition of intestinal flora, metabolize more short-chain fatty acids, reduce a uric acid level, maintain synthesis of insulin, avoid rising of a blood sugarlevel, and achieve the purpose of relieving type 2 diabetes.

Description

technical field [0001] The invention belongs to the technical field of microbial medicines, and in particular relates to the use of a secondary metabolite of Aspergillus terreus-butyrolactone in preparing medicines for treating diabetes. Background technique [0002] In the nearly one hundred years of research on terrestrial microorganisms, a large number of natural products with diverse chemical structures and significant biological activities have been discovered, which has greatly promoted the development of biotin drugs, such as: penicillin, vancomycin, streptomycin, etc. More and more attention to microorganisms naturally leads to repeated research on microorganisms, repeated development of known compounds, and a lower chance of discovering new compounds. Therefore, people began to pay attention to marine-derived microorganisms with larger microbial reserves and more diverse species. Due to their unique living environment (high pressure, high salt, low temperature, oli...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/365A61P3/10C12P17/04
CPCA61K31/365A61P3/10C12P17/04
Inventor 孙坤来张译文宫凯凯赵成英
Owner 嘉兴市爵拓科技有限公司
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