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Application of sulfadiazine in reproduction behaviors of trichogramma westwood infected with wolbachia

A technology of sulfadiazine and Trichogramma, which is applied in the field of arthropod reproductive mode regulation, can solve problems such as the regulation mechanism that remains to be studied, achieve good application value and promotion significance, and eliminate the effect of influence

Active Publication Date: 2019-07-12
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the Trichogramma short-tubular genome and its Wolbachia genome have been published, but the regulation mechanism of Wolbachia on Trichogramma parthenogenesis remains to be studied

Method used

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  • Application of sulfadiazine in reproduction behaviors of trichogramma westwood infected with wolbachia
  • Application of sulfadiazine in reproduction behaviors of trichogramma westwood infected with wolbachia
  • Application of sulfadiazine in reproduction behaviors of trichogramma westwood infected with wolbachia

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Experimental program
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Effect test

Embodiment 1

[0038] 1. Experimental method

[0039] Under the stereoscope, use a small writing brush to take out the short-tube Trichogramma females that have just emerged, put each head into the finger tube, and feed three kinds of antibiotic honey water respectively, and set up a control group. After feeding for 24 hours, put a lot of Parasitize on 100 fresh rice moth eggs that have been killed. After 24 hours of parasitism, use a small brush to pick out Trichogramma and put it in a centrifuge tube for storage at -30°C. Put the egg card into a finger tube and continue to put it in a climate box for cultivation. The temperature is 25°C, the relative humidity is 80% to 85%, and the light condition is L :D=14:10. Three sets of repetitions were performed for each treatment. Continuously treat 2 generations, record the number of eggs that turn black in each generation, that is, the number of eggs laid by Trichogramma, the number of females, males, and male and female mosaics, and calculate ...

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Abstract

The invention discloses application of sulfadiazine in reproduction behaviors of trichogramma westwood infected with wolbachia, and particularly provides application of the sulfadiazine in removing endosymbiotic wolbachia or reducing titer of the wolbachia in animal bodies. It is found for the first time that the wolbachia in the trichogramma westwood can be well removed after the trichogramma westwood is fed with honey water containing 1.0mg / ml of the sulfadiazine, the method is convenient and time saving, and holandric offsprings are obtained. The application lays a foundation for further utilization of the wolbachia in regulation of the reproductive mode of arthropods, and has high application value and promotion significance.

Description

technical field [0001] The invention relates to the technical field of arthropod reproductive pattern regulation, and more specifically, relates to the application of sulfadiazine pairs in the reproductive behavior of Trichogramma infecting Wolbachia. Background technique [0002] Wolbachia is a common intracellular symbiotic bacteria with a high infection rate in insects, mites and other arthropods and nematodes. The study shows that, based on the reported data, it is estimated by the model that 66% of the arthropods in nature are infected with Wolbachia. During the long-term evolution process, Wolbachia has the ability to regulate the host's reproductive mode in the following ways: cause the host's cytoplasm to be incompatible, induce parthenogenesis, cause feminization of male genes, kill males, and enhance fecundity to benefit its own Survival and spread, but have a negative impact on the reproduction of the host. [0003] Trichogramma Westwood is a parasitic insect be...

Claims

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Application Information

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IPC IPC(8): A01K67/033A61K31/635A61P31/04A01N43/54A01P1/00
CPCA01K67/033A01N43/54A61K31/635A61P31/04
Inventor 何晓芳张美娇王轩李远玉
Owner SOUTH CHINA AGRI UNIV
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