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Laser-induced fluorescence detection system capable of realizing visual real-time imaging and focusing

A laser-induced fluorescence, real-time imaging technology, applied in the field of analysis and detection, can solve the problems of difficult cleaning of fluorescent reagents, long-time calibration operation, affecting sample detection, etc.

Active Publication Date: 2019-07-09
BEIJING UNIV OF TECH
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Problems solved by technology

However, when the channel size is reduced to nanoscale, if the position of the detection window cannot be observed, it will take a long time to complete the calibration operation
In addition, chromatographic separation mostly uses chromatographic columns as the separation medium. According to the different forms of stationary phase, chromatographic columns are divided into packed columns, open-tubular columns, and monolithic columns. Since the chromatographic columns are filled with stationary phases or coated with walls, etc., fluorescent reagents may Problems such as cleaning difficulties affect the detection of samples

Method used

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  • Laser-induced fluorescence detection system capable of realizing visual real-time imaging and focusing
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  • Laser-induced fluorescence detection system capable of realizing visual real-time imaging and focusing

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Embodiment 1

[0028] Such as figure 1As shown, it is a schematic structural diagram of a laser-induced fluorescence detection system for visual real-time imaging focusing, an LED lamp (13), an optical filter (14), a diaphragm (15), an objective lens (5), and a first-two sensor with a cut-off wavelength of 488nm. The dichroic mirror (6), the second dichroic mirror (7) with a cutoff wavelength of 600nm, and the reflection mirror (16) are on the same longitudinal axis. An optically pumped semiconductor laser with an excitation wavelength of 488nm (1), a neutral density filter (2), a laser filter with a central wavelength of 488nm, a cylindrical lens (4) and a first dichroic mirror with a cutoff wavelength of 488nm (6) On the same horizontal axis, the laser beam emitted by an optically pumped semiconductor laser (1) with an excitation wavelength of 488nm is purified by a laser filter (3) with a center wavelength of 488nm attenuated by a neutral density filter (2), Then through the cylindrical ...

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Abstract

The invention discloses a laser-induced fluorescence detection system capable of realizing visual real-time imaging and focusing, and belongs to the technical field of analysis and detection. The detection system adopts a confocal structure, and comprises a laser light path, a fluorescence collection light path and an imaging calibration light path. The laser light path is formed by a laser, a neutral density filter, a laser filter, a cylindrical lens, a dichroscope and an objective lens; the fluorescence collection light path is formed by the objective lens, a dichroscope, a fluorescence filter, a lens, a slit and a silicon avalanche photodiode; and the imaging calibration light path is formed by a light-emitting diode (LED), an optical filter, a diaphragm, an objective lens, dichroscopes, a reflector, a lens and a camera. Through the imaging calibration light path, a focusing step of a capillary tube and a chip in the application of the laser-induced fluorescence detection system isreduced, and visual adjustment and real-time on-line display of a detection window and a laser light spot are realized. The detection system is high in sensitivity and good in stability, and the detection of fluorescein sodium can reach a yocto mole level.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and in particular relates to a laser-induced fluorescence detection system for visual real-time imaging focusing. Background technique [0002] In the 1970s, Diebold and Zare first proposed the combination of laser-induced fluorescence detection (LIF) and high-performance liquid chromatography for the separation and analysis of aflatoxin B1, B2, G1 and G2. The use of laser as the excitation light source has its unique advantages. The laser has the characteristics of good monochromaticity and strong light concentrating. It can focus the beam to a very small area, and the photon flux is high, thus greatly improving the detection sensitivity. At present, the LIF detector is one of the most sensitive detectors. It is suitable for the detection of small volume samples and can be combined with various capillary or microchip separation techniques such as capillary liquid chromatography, c...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6456
Inventor 郭广生张文美刘蕾周颖燕蒲巧生汪夏燕
Owner BEIJING UNIV OF TECH
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