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Amino acid modified lignin broad-spectrum antibacterial agent as well as preparation method and application thereof

A lignin and amino acid technology, applied in the direction of antibacterial drugs, etc., can solve the problems of short circulation half-life, high toxicity of antibacterial peptides, high production costs, etc., and achieve the effects of reduced toxicity, good biocompatibility, and low price

Active Publication Date: 2019-07-09
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the high toxicity, short circulating half-life (susceptible to protease hydrolysis) and high production cost of antimicrobial peptides limit their application

Method used

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  • Amino acid modified lignin broad-spectrum antibacterial agent as well as preparation method and application thereof
  • Amino acid modified lignin broad-spectrum antibacterial agent as well as preparation method and application thereof
  • Amino acid modified lignin broad-spectrum antibacterial agent as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 5 parts by weight of enzymatic lignin were dissolved in 20 parts by weight of an aqueous sodium hydroxide solution with a pH of 14. Subsequently, 0.5 parts by weight of glyoxal and 1.0 parts by weight of arginine were added, and heated to 60° C. with stirring. After reacting for 4 hours, the pH of the reaction solution was adjusted to 7.5 with hydrochloric acid, dialyzed to remove salt, and dried to obtain an amino acid-modified lignin broad-spectrum antibacterial agent.

[0041] The antibacterial activity of the prepared amino acid modified lignin broad-spectrum antibacterial agent was tested. First, an antiseptic is dissolved in sterile water. Then, serial dilutions were performed in 96-well plates with Muller-Hinton broth. Second, the bacterial suspension was grown in Mueller-Hinton broth. Further serial dilutions were made to final assay concentrations in 96-well plates. In addition, two control groups were set up: (1) pure bacteria + culture solution (without l...

Embodiment 2

[0044] 0.5 parts by weight of alkali lignin is dissolved in 10 parts by weight of an aqueous sodium hydroxide solution at pH=14. Subsequently, 0.2 parts by weight of succinaldehyde and 1.2 parts by weight of histidine were added, and heated to 80° C. with stirring. After reacting for 2 hours, the pH of the reaction solution was adjusted to 6 with hydrochloric acid, desalted by centrifugal ultrafiltration, and dried to obtain an amino acid-modified lignin broad-spectrum antibacterial agent.

[0045] Using the same antibacterial test and cytotoxicity test as in Example 1, the experimental results are basically the same as in Example 1.

Embodiment 3

[0047] 2 parts by weight of solvent-type lignin are dissolved in 15 parts by weight of aqueous sodium hydroxide solution with pH=13. Subsequently, 0.5 parts by weight of formaldehyde and 1.5 parts by weight of lysine were added, and heated to 50° C. with stirring. After reacting for 4 hours, the pH of the reaction solution was adjusted to 7 with hydrochloric acid, desalted by centrifugal ultrafiltration, and dried to obtain an amino acid-modified lignin broad-spectrum antibacterial agent.

[0048] Using the same antibacterial test and cytotoxicity test as in Example 1, the experimental results are basically the same as in Example 1.

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Abstract

The invention belongs to the technical field of natural polymer antibacterial agents, and discloses an amino acid modified lignin broad-spectrum antibacterial agent as well as a preparation method andapplication thereof. The method provided by the invention comprises the following steps: adding 1-5 parts by weight of aldehyde and 1-5 parts by weight of amino acid into an akali solution containing5-50 parts by weight of lignin, conducting heating for reaction at 40-120 DEG C for 1-6 hours, so as to obtain the amino acid modified lignin broad-spectrum antibacterial agent. The invention also provides the amino acid modified lignin broad-spectrum antibacterial agent prepared by the method. According to the method disclosed by the invention, lignin is modified by an active amino acid component in a natural antibacterial peptide, so that the amino acid modified lignin broad-spectrum antibacterial agent is obtained, the antibacterial activity of the lignin is effectively enhanced, the lignin has high antibacterial activity, and the antibacterial property is superior to that of common lignin; meanwhile, the agent has an inhibition effect on gram-positive bacteria and gram-negative bacteria, and has broad-spectrum antibacterial property; and the agent is low in toxicity and good in biocompatibility, and has a huge application prospect in the fields of biological medicines, foods and cosmetics.

Description

technical field [0001] The invention belongs to the technical field of natural polymer antibacterial agents, in particular to an amino acid modified lignin broad-spectrum antibacterial agent and its preparation method and application. Background technique [0002] Worldwide, bacterial infections remain the leading cause of death. In order to prevent and control the high morbidity and mortality caused by bacterial infections, researchers have been devoting themselves to the development of highly effective antimicrobial materials / surfaces that can eliminate bacteria before they become dangerous. [0003] Since the discovery of penicillin by Alexander Fleming in 1923, the age of antibiotics has entered. However, nearly a century of unrestricted use of antibiotics has severely diminished their effectiveness. Over the past 20 years, the phenomenon of bacterial antimicrobial resistance (AMR) has been on the rise all over the world. In 2014, a document describing the situation o...

Claims

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Application Information

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IPC IPC(8): C08H7/00A61P31/04
CPCA61P31/04C08H6/00
Inventor 邱学青李岚钱勇陈凯杨东杰楼宏铭
Owner SOUTH CHINA UNIV OF TECH
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