Preparation method of pre-activated polystyrene microsphere

Abstract

The invention discloses a preparation method of a pre-activated polystyrene microsphere, which takes styrene as a monomer, polyvinylpyrrolidone as a dispersion stabilizer, 30% ethanol aqueous solutionas a dispersion medium, azobisisobutyronitrile as an initiator, and uses a dispersion polymerization process to prepare the polystyrene microsphere, then polystyrene microspheres are added to the microsphere activation solution prepared by ethanesulfonic acid, carbodiimide and N-hydroxy succinimide, so as to prepare the pre-activated polystyrene microspheres. The pre-activated polystyrene microspheres prepared by the invention have small particle size and are suitable for binding to proteins such as antibodies or antigens. The surface of the microspheres is activated and has adsorption or chemical bonding ability and good stability. The microspheres can be well adsorbed and bound to proteins such as antibodies or antigens without prior activation treatment before use and are suitable formass production.

Description

technical field [0001] The invention relates to the field of polymer chemistry, in particular to a preparation method of preactivated polystyrene microspheres. Background technique [0002] Surface-functionalized polymer microspheres are easy to react with enzymes, proteins, nucleic acids, etc. due to the presence of active groups such as carboxyl, amino, or hydroxyl groups on the surface, making them widely used in biology, chemistry, materials, and medicine. At present, the preparation of monodisperse functionalized polymer microspheres with controllable particle size has become a research hotspot in the field of polymer materials. Polymer microspheres have the characteristics of large specific surface area, strong adhesion, strong agglutination and strong surface reaction ability, and have broad application prospects. Polymer microspheres with uniform micron-sized particles, as functional polymer materials, have a wide range of applications in analytical chemistry, bioch...

AI Technical Summary

Problems solved by technology

In this process, the activation efficiency is not easy to control; the dispersion process generally uses methods such as ultrasound, press filtration, ultrafiltration, etc., but they are all subject to the capacity of the equipment, and the production capacity of each batch is limited.