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Primer for identifying seed purity of Jinyan brilliant green No.1 brassica chinensis and application of primer

A technology of pakchoi and seeds, applied in the biological field, can solve problems such as being easily affected by the environment, heavy workload, and backlog of seed stocks, and achieve the effect of efficient and accurate quality control

Active Publication Date: 2019-06-28
TIANJIN RES INST OF VEGETABLE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional method of seed purity identification uses direct observation of the characteristics and consistency of specific growth and development stages of crops in the field. There are factors such as long cycle, heavy workload and easy to be affected by the environment, which often conflict with the peak sales season of seeds, resulting in seed Overstock

Method used

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  • Primer for identifying seed purity of Jinyan brilliant green No.1 brassica chinensis and application of primer
  • Primer for identifying seed purity of Jinyan brilliant green No.1 brassica chinensis and application of primer
  • Primer for identifying seed purity of Jinyan brilliant green No.1 brassica chinensis and application of primer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Materials

[0024] Three individual plants of the male parent material, the female parent material, and the Jinyan Kuailv No. 1 hybrid were used for primer screening. 92 seeds of Jinyan Kuailv No. 1 hybrid produced by 6 contracted farmers in the seed production base were randomly selected for seed purity identification.

[0025] 2. Screening of SSR primers

[0026] According to the resequencing data of the Chinese cabbage genome, multiple pairs of primers were designed to detect the polymorphism of Jinyan Kuailv 1, the female parent material, and the male parent material, and the primer pair A09-17 with co-dominant differences was screened out. The sequence is as follows :

[0027] F: 5'-TCCTCGAGTTTCGCTTCATATCCTG-3' (SEQ ID NO: 1)

[0028] R: 5'-AGTCTGGAAACTTACATGGAAGTAAAATACAAAAC-3' (SEQ ID NO:2)

[0029] The marker has good reproducibility and clear bands. Using this primer as a specific primer, the genomic DNA of the sample to be tested is used as a template f...

Embodiment 2

[0044] Comparative test:

[0045] The Jinyan Kuailv No. 1 commercial variety to be tested produced by 6 contracted farmers in the seed production base was randomly selected about 100 seeds from each household, and the field was dug in the open field, and sowed at a density of 200 plants per square meter. After sowing, about 3 All seedlings emerge from the sky, and when they grow to the stage of 7 leaves and 1 heart, according to the performance characteristics of the samples to be tested, such as plant height, leaf color, leaf shape, petiole color, length, and width, whether they conform to the unity of Jinyan Kuailv No. 1 commercial variety and consistency, judge whether it is Jinyan Express No. 1 commercial variety, and count the seed purity of the samples to be tested.

[0046]

[0047] Conclusion: The identification of the purity of Jinyan Kuailv No. 1 seeds by the SSR molecular marker primers provided by the present invention is consistent with the identification of se...

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Abstract

The invention discloses an SSR marker primer for identifying seed purity of Jinyan brilliant green No.1 brassica chinensis of which the nucleotide sequence is shown in SEQ ID NO: 1-2. The invention further discloses a method for identifying the coeno-species seed purity of the Jinyan brilliant green No.1 brassica chinensis. The specific primer and method can complete the seed purity identificationof Jinyan brilliant green No.1 coeno-species in a laboratory for 3-5 hours, have the advantages of being rapid and stable and not being affected by the environment, can replace a traditional field seed purity identification method, and are conductive to the efficient and accurate quality control of the Jinyan brilliant green No.1 seeds.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to an SSR marker primer for identifying the purity of Jinyan Kuailv No. 1 pakchoi seeds and its application. Background technique [0002] Chinese cabbage ( Brassica rapa L. ssp. chinensis ) belongs to Brassica genus of Brassicaceae and belongs to non-heading cabbage, which is rich in protein, sugar, dietary fiber, carotene, vitamins, calcium, phosphorus, iron and so on. Compared with traditional Chinese cabbage, pakchoi has a short growth cycle and can be planted and marketed almost all year round. It has less fiber content, soft texture, sweet and fragrant taste, and is edible from seedlings to adult plants. It is the largest planted area in my country. One of the largest and most widely distributed popular vegetables, it plays an important role in ensuring the supply of vegetables in our country. [0003] High-quality varieties are the key to ensure the normal production and supply...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
Inventor 王超楠张红华德平黄志银李梅范伟强张斌刘晓晖
Owner TIANJIN RES INST OF VEGETABLE
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