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Mass spectrometric detection method based on paper target

A technology of mass spectrometry detection and target film, which is applied in the field of laser analysis ionization-mass spectrometry detection, can solve problems such as the inability to realize rapid sample detection, and achieve the effect of improving reproducibility and speed of detection

Inactive Publication Date: 2019-06-25
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] LDI has many advantages in the analysis of compounds, but due to the requirements of the instrument, the rapid detection of samples cannot be realized

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Paper-based mass spectrometry methods:

[0026] 1. Prepare an aqueous solution of 1.0 mg / mL gold nanocluster material as matrix solution;

[0027] 2. Ultrasonic the above system at room temperature for 1-2 minutes to form a uniform solution system;

[0028] 3. Prepare 1.0mg / mL lactose sample aqueous solution as the sample solution

[0029] 4. Add the sample solution dropwise onto the paper target of the filter paper, and wait for the solution to dry;

[0030] 5. Add the matrix solution dropwise to the paper target sheet with the lactose sample obtained in step 4), so that the volume ratio of the matrix solution to the sample is 1:1, and dry the solution;

[0031] 6. Perform LDI MS detection on the dried paper target.

[0032] All LDI MS analyzes were performed on an AB Sciex 5800 time-of-flight mass spectrometer.

[0033] Analysis result: by figure 2 A shows that lactose can be detected by LDI MS, and its signal intensity reaches 10 4 The results are comparable t...

Embodiment 2

[0035] Paper-based mass spectrometry methods:

[0036] 1. Prepare an aqueous solution of 0.5 mg / mL gold nanocluster material as matrix solution;

[0037] 2. Ultrasonic the above system at room temperature for 1-2 minutes to form a uniform solution system;

[0038] 3. Prepare 1.0mg / mL lactose sample solution as the sample solution

[0039] 4. Add the sample solution dropwise onto the paper target of the filter paper, and wait for the solution to dry;

[0040] 5. Add the matrix solution dropwise to the paper target sheet with the lactose sample obtained in step 4), so that the volume ratio of the matrix solution to the sample is 1:1, and dry the solution;

[0041] 6. Perform LDI MS detection on the dried paper target.

[0042] All LDI MS analyzes were performed on an AB Sciex 5800 time-of-flight mass spectrometer.

[0043] Analysis result: by figure 2 B shows that lactose can be detected by LDI MS with a signal intensity of 10 4 The results are comparable to those of conv...

Embodiment 3

[0045] Paper-based mass spectrometry methods:

[0046] 1. Prepare an aqueous solution of 1.0mg / mL mesoporous carbon as matrix solution;

[0047] 2. Ultrasonic the above system at room temperature for 1-2 minutes to form a uniform solution system;

[0048] 3. Prepare 1.0mg / mL lactose sample solution as the sample solution

[0049] 4. Add the sample solution dropwise onto the paper target of the filter paper, and wait for the solution to dry;

[0050] 5. Add the matrix solution dropwise to the paper target sheet with the lactose sample obtained in step 4), so that the volume ratio of the matrix solution to the sample is 3:1, and the solution to be dried is dried;

[0051] 6. Perform LDI MS detection on the dried paper target.

[0052] All LDI MS analyzes were performed on an AB Sciex 5800 time-of-flight mass spectrometer.

[0053] Analysis results: by figure 2 C shows that lactose can be detected by LDI MS with a signal intensity of 10 4 The results are comparable to those ...

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Abstract

The invention relates to a mass spectrometric detection method based on a paper target. The method uses paper (filter paper, printing paper and the like) as a sample target. Laser analytical ionization and mass spectrometric detection of sample molecules are realized with the aid of a conventional organic matrix or a gold cluster nanomaterial. According to the invention, the sample carrying function of the paper target and the auxiliary ionization function of an organic or inorganic matrix on the sample are combined; and the method has the advantages of convenient sampling, easy sample storageand transportation, high detection sensitivity and the like, effectively avoids the 'coffee ring' effect of the organic matrix, and is suitable for fast, high-sensitivity and high throughput detection of a variety of biomolecules, drug molecules, environmental pollutant molecules (sugars, nucleosides, natural products, basic drugs, peptides and the like).

Description

technical field [0001] The invention relates to rapid mass spectrometry analysis of biological samples, medicines, environmental pollutants, etc., in particular to a laser analysis ionization-mass spectrometry detection method using paper as a bearing target. Background technique [0002] Laser desorption ionization (LDI) technology is an analysis technology that uses organic substrates with ultraviolet light absorption or some solids to absorb laser energy to achieve laser desorption ionization of the analyte. It has the characteristics of label-free, high-throughput and automation, and realizes Analysis of non-volatile samples. At the same time, compared with other mass spectrometers, MALDI has the advantages of higher resistance to salt, buffer and other non-volatile components. Since most sample molecules are dissociated into single-charged molecular ions in MALDI analysis, the mass spectrum obtained is relatively simple, which is beneficial to spectrum analysis. [00...

Claims

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Application Information

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IPC IPC(8): G01N27/62
Inventor 王方军李吉楠
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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