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Method for simultaneously detecting a plurality of polypeptide drugs in egg

A multi-peptide, egg technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem of inability to detect the residues of various polypeptide drugs in eggs at the same time

Inactive Publication Date: 2019-06-21
HUBEI INSPECTION & QUARANTINE TECH CENT
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] For this reason, the embodiment of the present invention provides a method for simultaneously detecting multiple polypeptide drugs in eggs to solve the problem in the prior art that it is impossible to simultaneously detect the residues of multiple polypeptide drugs in eggs

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  • Method for simultaneously detecting a plurality of polypeptide drugs in egg
  • Method for simultaneously detecting a plurality of polypeptide drugs in egg
  • Method for simultaneously detecting a plurality of polypeptide drugs in egg

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Embodiment Construction

[0025] The implementation mode of the present invention is illustrated by specific specific examples below, and those who are familiar with this technology can easily understand other advantages and effects of the present invention from the contents disclosed in this description. Obviously, the described embodiments are a part of the present invention. , but not all examples. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0026] The embodiment of the present invention provides a detection method for 5 kinds of polypeptide drug test substances in eggs, the test substances mainly include 5 kinds of polypeptide medicines including enramycin A, enramycin B, bacitracin, Vancomycin, virginiamycin M1. The detection method has the characteristics of fast, accurate, simple, effective and high sensitivity for the pretreatm...

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Abstract

The embodiment of the invention discloses a method for simultaneously detecting a plurality of polypeptide drugs in an egg, which comprises the steps of: mixing a mixed solution composed of methanol,acetonitrile and formic acid with an egg sample; performing centrifugation to obtain a supernatant containing an object to be detected; purifying the supernatant containing the object to be detected by a Qasis PRiME HLB solid phase extraction column; collecting a liquid; and after drying and re-dissolving, detecting the type and the content of the object to be detected in the filtered filtrate byliquid chromatography mass spectrometry. In the embodiment of the invention, the test sample is extracted by using the mixed solution composed of methanol, acetonitrile and 1% formic acid as an extracting agent, thereby effectively precipitating the protein, releasing the bonded residue and extracting the target drug. Then, the Qasis PRiME HLB solid phase extraction column is used to remove fats and phospholipids by a purification process. The extraction method has high extraction efficiency, short pretreatment time, a good purification effect and a high recovery rate.

Description

technical field [0001] The embodiment of the present invention relates to the technical field of food safety, in particular to a method for simultaneously detecting multiple polypeptide drugs in eggs. Background technique [0002] Peptide drugs are currently widely used as feed additives such as poultry, mainly for promoting animal growth and treating livestock and poultry diseases. Polypeptide antibiotics are small molecular peptides formed by condensation of different types of amino acids. Commonly used polypeptide antibiotics include enramycin, bacitracin, vancomycin, and virginiacin. With the widespread use of polypeptide antibiotics in the poultry breeding process, abuse and non-compliance with withdrawal periods have occurred from time to time, and the residues of polypeptide drugs in eggs have attracted increasing attention. Drugs may enter the human body through the food chain and endanger human health. If exposed for a long time, there is a potential risk of increa...

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 叶诚王鹏赵晓亚付晓芳王晗廖小丽钱章
Owner HUBEI INSPECTION & QUARANTINE TECH CENT
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