gRNA for targeting HBB RNA, HBB mutation detection method and detection kit based on C2c2

A c2c2, gene technology, used in DNA/RNA fragments, other methods of inserting foreign genetic material, DNA preparation, etc.

Pending Publication Date: 2019-06-21
GUANGZHOU PLUSLIFE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0015] The applicant found that there is no relevant report on the detection method of β-globin gene (HBB) mutation based on CRISPR-C2c2 system

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  • gRNA for targeting HBB RNA, HBB mutation detection method and detection kit based on C2c2
  • gRNA for targeting HBB RNA, HBB mutation detection method and detection kit based on C2c2
  • gRNA for targeting HBB RNA, HBB mutation detection method and detection kit based on C2c2

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Embodiment Construction

[0109] The following descriptions are preferred implementations of the embodiments of the present invention. It should be pointed out that those skilled in the art can make some improvements and modifications without departing from the principles of the embodiments of the present invention. These improvements And retouching are also regarded as the scope of protection of the embodiments of the present invention.

[0110] Unless otherwise specified, the reagents and consumables used in the examples of the present invention are commercially available.

[0111] The practice of the present invention employs, unless otherwise indicated, conventional techniques of immunology, biochemistry, chemistry, molecular biology, microbiology, cell biology, genomics and recombinant DNA, which are within the skill of the art. See Sambrook, Fritsch, and Maniatis, MOLECULAR CLONING: A LABORATORY MANUAL, 2nd ed. (1989); Contemporary Molecular Biology Laboratory Manual (CURRENT PROTOCOLS IN MOLECU...

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Abstract

The invention provides gRNA for specifically targeting HBB RNA, and further provides a human beta globin gene (HBB) mutation detection method and a detection kit based on a regularly spaced cluster short palindrome repeat sequence (CRISPR)-C2c2 system. The invention provides a detection method, which integrates the advantages of gRNA in targeted recognition of an HBB gene transcription product RNA(target RNA sequence) and the characteristics that when CRSPR-C2c2 complex detects target RNA sequence, the complex cuts the report RNA with a detection mark and release a detectable signal. The CRSPR-C2C2 system is applied to mutation detection of beta globin gene (HBB), the method and the kit have high sensitivity and accuracy, and are a detection method and a detection kit with great commercial application value.

Description

technical field [0001] The invention relates to the field of gene detection and gene modification, and relates to a gRNA for specifically targeting HBB RNA, and a β-specific protein gene (HBB) mutation based on a clustered regularly interspaced short palindromic repeat sequence (CRISPR) system Detection methods, detection kits. Background technique [0002] Clustered regularly interspaced short palindromic repeat system (clustered regularly interspaced shortpalindromic repeat; CRISPR-associated, CRISPR-Cas) is an important immune defense system for archaea and bacteria to resist virus and plasmid infection, and is used to resist foreign genetic material. Invasions, such as phage viruses and foreign plasmids. At the same time, it provides acquired immunity for bacteria: this is similar to the acquired immunity of mammals. When bacteria are invaded by viruses or foreign plasmids, they will generate corresponding "memory" to resist their re-invasion. The CRISPR / Cas system can...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12N15/90C12N15/85C12N15/10C12Q1/6827
Inventor 陈翀罗镇华
Owner GUANGZHOU PLUSLIFE TECH CO LTD
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