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Preparation method and application of a three-dimensional chip for screening components of traditional Chinese medicine

A technology of traditional Chinese medicine components and three-dimensional chips, which is applied to chemical instruments and methods, analytical materials, and laboratory containers, etc., can solve the problems of poor specificity of small molecule array chips and low immobilization capacity of small drug molecules, and achieve improved Fixed amount, guaranteed uniformity, and solved the effect of poor specificity

Active Publication Date: 2021-04-13
QIANNAN NORMAL UNIV FOR NATTIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, when the small molecule array chip technology is used for high-throughput screening of traditional Chinese medicine components containing different small molecule structures, the low immobilization capacity of small drug molecules and the poor specificity of the small molecule array chip have become the technical bottlenecks.

Method used

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  • Preparation method and application of a three-dimensional chip for screening components of traditional Chinese medicine
  • Preparation method and application of a three-dimensional chip for screening components of traditional Chinese medicine
  • Preparation method and application of a three-dimensional chip for screening components of traditional Chinese medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Add deionized water, 25% ammonia water and 30% hydrogen peroxide into the chip cleaner, wherein the volume ratio of deionized water, ammonia water and hydrogen peroxide is 5:1:1, and then put it on the surface For glass substrates coated with gold film, heat up to 50°C to clean the substrate for 30 minutes, then rinse with deionized water and ethanol, and dry with nitrogen gas to complete the cleaning;

[0034] (2) Soak the cleaned substrate obtained in step (1) in 1mM 2-mercaptoethylamine ethanol solution, place it at room temperature for 15 hours, self-assemble a monomolecular layer on the surface of the substrate, and then assemble the surface with 2 - The substrate of the mercaptoethylamine monolayer is soaked in the N,N-dimethylformamide (DMF) solution containing triethylamine and 2-bromopropionyl bromide, reacted at -5°C for 3 hours, and then React at room temperature for 8 hours, then clean the chip to obtain a substrate with an initiator attached to the surf...

Embodiment 2

[0037] (1) Add deionized water, 25% ammonia water and 30% hydrogen peroxide into the chip cleaner, wherein the volume ratio of deionized water, ammonia water and hydrogen peroxide is 5:1:1, and then put it on the surface For the glass substrate coated with gold film, heat up to 70°C to clean the substrate for 10 minutes, then rinse with deionized water and ethanol, and blow dry with nitrogen to complete the cleaning;

[0038] (2) Soak the cleaned substrate obtained in step (1) in 5mM 2-mercaptoethylamine ethanol solution, place it at room temperature for 10 hours, self-assemble a monomolecular layer on the surface of the substrate, and then assemble the surface with 2 - The substrate of the mercaptoethylamine monolayer is soaked in the N,N-dimethylformamide (DMF) solution containing triethylamine and 2-bromopropionyl bromide, reacted at 5°C for 2 hours, and then at room temperature The reaction was carried out for 5 hours, and then the chip was cleaned to obtain a substrate wi...

Embodiment 3

[0041](1) Add deionized water, 25% ammonia water and 30% hydrogen peroxide into the chip cleaner, wherein the volume ratio of deionized water, ammonia water and hydrogen peroxide is 5:1:1, and then put it on the surface For glass substrates coated with gold film, heat up to 60°C to clean the substrate for 20 minutes, then rinse with deionized water and ethanol, and blow dry with nitrogen to complete the cleaning;

[0042] (2) Soak the cleaned substrate obtained in step (1) in 2mM 2-mercaptoethylamine ethanol solution, place it at room temperature for 12 hours, self-assemble a monomolecular layer on the surface of the substrate, and then assemble the surface with 2 - The substrate of the mercaptoethylamine monolayer is soaked in the N,N-dimethylformamide (DMF) solution containing triethylamine and 2-bromopropionyl bromide, and reacted at 0°C for 2.5 hours, then at room temperature The reaction was carried out for 6 hours, and then the chip was cleaned to obtain a substrate with...

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Abstract

The invention discloses a preparation method and application of a three-dimensional chip for screening traditional Chinese medicine components. The chip includes a support layer, an initiator layer and a polymer brush layer; the initiator layer is covalently anchored on the support layer through a metal-sulfur bond; and the polymer brush layer is covalently bonded to the initiator layer. The three-dimensional chip for screening Chinese medicine components of the present invention can fix small drug molecules or Chinese medicine components with different structures; in addition, the chip has a three-dimensional structure and introduces strong anti-non-specific monomers into the polymer layer, so that the Chinese medicine components can be screened in three dimensions The chip not only greatly improves the immobilization amount of small drug molecules or components of traditional Chinese medicine, but also significantly enhances the sensing signal of the chip, and also solves the problem of poor specificity of the small molecule array chip.

Description

technical field [0001] The invention relates to the field of biochip preparation, in particular to a preparation method and application of a three-dimensional chip for screening components of traditional Chinese medicine. Background technique [0002] Now to clarify the active ingredients of drugs, we must use modern drug screening technology. Drug screening is to apply appropriate methods and techniques to natural products or synthetic compounds that may be used as drugs to detect their possible pharmacological activities. Applying the latest technology of modern drug screening to the research of traditional Chinese medicine will greatly promote the modernization of traditional Chinese medicine research, such as high-throughput drug screening technology. Today, high-throughput screening technology has become the core technology for theoretical research on structures and drug development by pharmaceutical companies, and small molecule array chips are the most widely used. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00G01N21/552
Inventor 彭开美戴学新杨再波黄德娜杨艳毛海立邹洪涛
Owner QIANNAN NORMAL UNIV FOR NATTIES
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