A method for constructing a molecular semi-adder and a semi-subtracter

Abstract

The invention discloses a method for constructing a molecular semi-adder and a molecular semi-subtracter, and belongs to the technical field of molecular computers. The method is characterized in thata Cut Smart buffer solution and a plurality of DNA double chains are taken to construct a logic operation system; two kinds of incision enzymes Nb.BtsI and Nt.BbvCI are used as two paths of parallelinput signals; under the specific cutting action of the cutting endonuclease, the cutting and endonucleating are carried out. According to the half adder and the half subtracter constructed by the method, molecular devices are simple in structure, easy to implement, high in sensitivity, fast in reaction speed, obvious in reaction trend and very low in leakage rate, the output signals are easy to detect, the fluorescent signals of multiple colors clearly represent different signal outputs, and in addition, the method can also be used for accurately detecting the incision enzyme.

Description

technical field [0001] The invention belongs to the technical field of molecular computers, and in particular relates to a method for constructing molecular half-adders and half-subtractors. Background technique [0002] A logic circuit is a kind of transmission and specific processing of discrete signals. It uses binary logic operations as the basic principle to realize logic operations and operations on digital signals. In today's electronic computers, logic circuits are composed of a large number of transistors, which are characterized by high and low levels representing 1 and 0 in binary signals. Simple logic circuits include AND logic gates, OR logic gates, XOR logic gates, NOR logic gates, etc. In addition to simple logic operations, they can also be combined into more complex logic circuits by cascading and parallel connections. Logic circuits such as half-adders and half-subtractors are the basic components of the arithmetic logic operation unit that constitutes the...

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Problems solved by technology

[0008] In the prior art, there are methods for constructing molecular half-adders and half-subtractors based on the structure of regulatory ribozymes, and for constructing molecular half-adders and half-adders based on the G tetramer structure. The method of subtractor, the method of constructing molecular half-adder and half-subtractor based on DNA strand replacement reaction, etc., although the logic operation function of half-adder and half-subtractor is well realized, the experimental reaction time is long, and the general time In 3 to 5 hours, the response time is too long, which means that it will be affected by interference for a long time, and the system fault tolerance will be reduced.

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