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Composite microbial inoculum with growth-promoting and yield-increasing effects on capsicum and application thereof

A technology of composite bacteria and growth-promoting bacteria, which is applied in the field of agricultural microorganisms to achieve the effects of promoting the growth of peppers, reducing the use of chemical fertilizers, and increasing the yield of peppers

Active Publication Date: 2019-06-14
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the yield-increasing effects were all carried out under the application rate of conventional chemical fertilizers, and it was only a field test of the compound bacterial agent on one pepper variety

Method used

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  • Composite microbial inoculum with growth-promoting and yield-increasing effects on capsicum and application thereof
  • Composite microbial inoculum with growth-promoting and yield-increasing effects on capsicum and application thereof
  • Composite microbial inoculum with growth-promoting and yield-increasing effects on capsicum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: Molecular identification of 3 bacterial strains forming composite bacterial agent

[0030] Molecular identification of the three strains: Bacterial DNA was extracted from the strains HGD12, HGD3, and HP9, respectively, using the DNA as a template, using bacterial 16S rDNA universal primers for PCR amplification, and sequencing the amplified products. The sequencing results of the three strains of bacteria As shown in the sequence table, the amplified lengths of the 16S rDNA sequences were 1417, 1442, and 1454 bp, respectively. After homology comparison, the 16S rDNA sequence of HGD12 strain was 99.93% homologous to the Bacillus flexus NBRC15715 16S rDNA sequence (NCBI accession number: BCVD01000224) in the GenBank database; The sequence (NCBI accession number: CP018846) has a homology of 99.86%; the 16S rDNA sequence of HP9 strain has a homology of 99.86% with Bacillus velezensis CR502 (Genbank accession number: AY603658) in the GenBank database.

[0031]...

Embodiment 2

[0037] Embodiment 2: Determination of the growth-promoting characteristics of 3 bacterial strains that form composite microbial agent

[0038] Quantitative determination of phosphate dissolution: transfer the activated bacterial solution to 100mL NBRIP liquid medium, culture it on a shaking table at 30°C for 7 days, take the culture solution and centrifuge it at 4°C, 10,000rpm for 10min, then measure the pH value, and use the molybdenum blue colorimetric method The soluble phosphorus content in the supernatant was measured, and the soluble phosphorus content in the blank control was deducted, which was the amount of phosphorus dissolved by each strain.

[0039] Quantitative determination of potassium solution: transfer the activated bacterial liquid to 50mL Alexander Rove liquid medium, shake the shaker culture at 30°C for 5 days, treat the culture liquid with hydrogen peroxide ashing method, and use atomic absorption method to determine The water-soluble potassium content in ...

Embodiment 3

[0050] Embodiment 3: to the preparation of the composite bacterial agent of capsicum growth-promoting and increasing production

[0051] Composition of the compound microbial agent for promoting growth and production of pepper: fermentation broth of Bacillus flexus HGD12, Pseudomonasputida HGD3 and Bacillus velezensis HP9 strains.

[0052] The above-mentioned preparation method of the compound microbial agent for promoting growth and increasing production of peppers comprises the following steps:

[0053] a. Slope activation: inoculate the preserved Bacillus flexus HGD12, Pseudomonas putida HGD3 and Bacillus velezensis HP9 strains on solid medium respectively, and activate the strains overnight;

[0054] b. Liquid activation: respectively pick the lawns of bacteria activated in step a in the liquid medium, and culture them on a shaker at 150 rpm at 28-30°C until the logarithmic growth phase;

[0055] c. Obtain the fermentation broth of each bacterial strain: inoculate the cul...

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Abstract

The invention discloses a composite microbial inoculum with growth-promoting and yield-increasing effects on capsicum and an application thereof. The composite microbial inoculum comprises fermentation liquor of Bacillus flexus HGD12, Pseudomonas putida HGD3 and Bacillus velezensis HP9 strains, wherein the Bacillus flexus HGD12 is Bacillus flexus and is preserved in China Center for Type Culture Collection on April 1, 2019, and the preservation number of the Bacillus flexus HGD12 is CCTCC NO: M 2019224; the Pseudomonas putida HGD3 is Pseudomonas putida and is preserved in the China Center forType Culture Collection on April 1, 2019, and the preservation number of the Pseudomonas putida HGD3 is CCTCC NO: M 2019223; and the Bacillus velezensis HP9 is preserved in the China Center for Type Culture Collection on April 8, 2019, and the preservation number of the Bacillus velezensis is CCTCC NO: 2019243. The composite microbial inoculum with the growth-promoting and yield-increasing effectson the capsicum and the application thereof have the advantages of promoting growth of the capsicum, improving yield of the capsicum, reducing application of chemical fertilizers and achieving reduction of the chemical fertilizer and increase of the yield of the capsicum.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and in particular relates to a microbial compound bacterial agent for promoting the growth and production of peppers and its application. Background technique [0002] my country is the world's largest producer and consumer of pepper, with an annual planting area of ​​about 1.333 million hectares. However, blind fertilization and excessive fertilization are common in pepper planting. The application of a large amount of chemical fertilizers leads to soil compaction and fertility decline, which can eventually cause The decline of pepper yield and quality has affected the sustainable development of agriculture. [0003] The strains in the microbial agent are non-toxic and harmless to the environment because they are derived from beneficial microorganisms in the soil or plant materials. After application, they can improve the soil, increase the content of organic matter in the soi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P21/00C12R1/07C12R1/40
CPCY02A40/10
Inventor 韩丽珍黄文茂王欢周静
Owner GUIZHOU UNIV
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