Enzyme-loaded filler and preparation method and application thereof

A spraying method and sol technology, applied in the field of enzyme catalysis, can solve the problems of insufficient activity and poor stability of enzyme-loaded fillers, and solve the problems of insufficient stability and poor activity of enzyme-loaded fillers, and achieve easy industrial application, activity and stability Enhanced performance and simple preparation process

Pending Publication Date: 2019-06-04
HEBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this method, the packing inside the tower body is first washed with sodium hydroxide solution to wash off the inactivated biological enzymes; then new sol enzymes are coated on the packing by bubbling from the bottom of the tower; The replacement method of the enzyme packing provides convenience, but does not give a solution to the insufficient stability and poor activity of the enzyme-loaded packing
[0006] Although the above-mentioned documents disclose some preparation methods of enzyme-loaded fillers, there are still problems of insufficient activity and poor stability of the prepared enzyme-loaded fillers. Therefore, a kind of enzyme-loaded fillers with better enzyme activity and excellent stability are developed. The preparation method is still of great significance

Method used

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  • Enzyme-loaded filler and preparation method and application thereof
  • Enzyme-loaded filler and preparation method and application thereof
  • Enzyme-loaded filler and preparation method and application thereof

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preparation example Construction

[0059] The schematic diagram of the preparation method of the enzyme-carrying filler of the present invention is as figure 1 Shown, as can be seen from the figure, the preparation method of described enzyme-carrying filler comprises the following steps:

[0060] (1) Fix the filler on the mechanical rotator with wire mesh, adjust the speed to make it rotate at a constant speed;

[0061] (2) Spray the enzyme-containing sol on the surface of the filler with a pneumatic spray gun on one side of the mechanical rotator, and dry it with a hair dryer on the other side to obtain the enzyme-loaded filler.

Embodiment 1

[0064] The preparation method of the enzyme-carrying filler of the present embodiment comprises the following steps:

[0065] (1) Use wire mesh to fix the metal Θ ring packing on the mechanical rotator, adjust the rotating speed to make it rotate at a constant speed;

[0066] (2) Utilize a spray gun to spray the enzyme-containing sol on the filler surface, the spraying process adopts "zigzag" spraying, and in the process of the spraying, a hair dryer is used to dry the surface of the metal Θ ring filler to obtain the enzyme-containing filler, and the spraying The air pressure is 0.15MPa, the flow rate of the spraying is 1.82kg / h, and the distance between the spray gun and the filler surface during the spraying process is 15cm.

[0067] The specification of the metal Θ ring packing used in this embodiment is 3cm×3cm, and the following examples and comparative examples all adopt the metal Θ ring packing of this specification.

[0068] The preparation method of the enzyme sol of...

Embodiment 2

[0074] The difference between this embodiment and embodiment 1 is that the air pressure of spraying is replaced by 0.2MPa, the flow rate of spraying is replaced by 2.05kg / h, the distance between the spray gun and the surface of the filler is replaced by 20cm, and other conditions are exactly the same as in embodiment 1 .

[0075] The enzyme-containing sol used in this example is the same as that in Example 1.

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Abstract

The invention relates to an enzyme-loaded filler and a preparation method and application thereof. The preparation method of the enzyme-loaded filler lies in that enzyme-containing sol is sprayed to the surface of the filler, so that the enzyme-loaded filler is obtained. Compared with the enzymic activity and the stability of an enzyme-loaded filler prepared by an impregnation method, the enzymicactivity and the stability of the enzyme-loaded filler prepared by the preparation method disclosed by the invention are obviously improved; after the enzyme-loaded filler is stored at room temperature for 50 days, the enzymic activity loss is smaller than 5%, and the enzymic activity for catalyzing the rectification process of a transesterification reaction of ethyl acetate and n-butanol is 599-621U / g.

Description

technical field [0001] The invention relates to the field of enzyme catalysis, in particular to an enzyme-carrying filler and its preparation method and application. Background technique [0002] Immobilized enzymes reduce the mobility of enzymes by physical or chemical methods. After the enzyme is immobilized, the general stability increases, it is easy to separate from the reaction system, and it is easy to control and can be used repeatedly. It is convenient for transportation and storage, and is conducive to automatic production, but the activity is reduced and the scope of use is reduced. [0003] CN105176962A discloses a method for immobilizing biological enzymes, specifically a method for immobilizing biological enzymes in ceramic fillers. The method includes: step 1, the process of treating biological enzymes; step 2, the process of treating ceramic fillers; step 3, the process of mixing the treated biological enzymes and ceramic fillers to prepare bioceramic fille...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12P7/62B05D1/02B05D3/04
Inventor 王洪海卢一凡刘文静许玉超陈二轩刘洪丽段宝轩尹依
Owner HEBEI UNIV OF TECH
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