Method for inhibiting tobacco black shank by combining bacterial fertilizer with fermentation bacterial solution

A technology of microbial bacterial fertilizer and tobacco black shank, which is applied in the direction of fertilization device, fertilizer mixture, application, etc., to achieve the effect of promoting plant absorption, curbing the occurrence of diseases and insect pests, and increasing the activity of beneficial microorganisms in the soil

Inactive Publication Date: 2019-06-04
云南省农业科学院生物技术与种质资源研究所 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is in order to solve the deficiency of existing adopting single antagonistic agent (liquid) to prevent and treat tobacco black shank technology

Method used

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  • Method for inhibiting tobacco black shank by combining bacterial fertilizer with fermentation bacterial solution
  • Method for inhibiting tobacco black shank by combining bacterial fertilizer with fermentation bacterial solution
  • Method for inhibiting tobacco black shank by combining bacterial fertilizer with fermentation bacterial solution

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Experimental program
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Effect test

Embodiment 1

[0025] The Bacillus cereus in the present invention has the following biological characteristics: the colonies protrude on the LB solid medium, the edges are smooth and fine, milky white, and the diameter is 1.1mm-1.8mm. See figure 1 , figure 2 .

[0026] 1. Preparation of fermentation broth

[0027] The solid-preserved Bacillus cereus (Bacillus cereus) is fermented by using the improved spore-producing liquid medium to obtain a fermented bacterial liquid. The number of effective viable bacteria in the fermentation broth is more than or equal to 100 million / g (cfu). The composition of the improved spore-producing liquid medium is as follows: glucose 1.5%, malt extract 2.5%, peptone 0.2%, NaCl 0.1%, (NH 4 ) 2 SO 4 0.1%, anhydrous CaCl 2 0.03%, ZnSO 4 ·7H 2 O 0.02%, FeCl 3 ·6H 2 O 0.002%, H 2 O 1000m1.

[0028] The liquid culture medium and the fermented bacteria liquid are prepared by the following method: Weigh the above-mentioned components respectively and diss...

Embodiment 2

[0037] 1. Preparation of fermentation broth

[0038] The solid-preserved Bacillus cereus (Bacillus cereus) is fermented by using the improved spore-producing liquid medium to obtain a fermented bacterial liquid. The number of effective viable bacteria in the fermentation broth is more than or equal to 100 million / g (cfu). The composition of the improved spore-producing liquid medium is as follows: glucose 1.5%, malt extract 2.5%, peptone 0.2%, NaCl 0.1%, (NH 4 ) 2 SO 4 0.1%, anhydrous CaCl 2 0.03%, ZnSO 4 ·7H 2 O 0.02%, FeCl 3 ·6H 2 O 0.002%, H 2 O 1000m1.

[0039] The liquid culture medium fermentation bacteria liquid is prepared by the following method: Weigh the above components and dissolve them in 1L of distilled water, adjust the pH to 7.0 with a pH meter after dissolving, put the above solutions in a triangular flask and put them in a sterilized The pot was sterilized at 121°C for 20 minutes. After the culture medium was cooled, 1ml of Bacillus cereus was tran...

Embodiment 3

[0048] 1. Preparation of fermentation broth

[0049] The solid-preserved Bacillus cereus (Bacillus cereus) is fermented by using the improved spore-producing liquid medium to obtain a fermented bacterial liquid. The number of effective viable bacteria in the fermentation broth is more than or equal to 100 million / g (cfu). The composition of the improved spore-producing liquid medium is as follows: glucose 1.5%, malt extract 2.5%, peptone 0.2%, NaCl 0.1%, (NH 4 ) 2 SO 4 0.1%, anhydrous CaCl 2 0.03%, ZnSO 4 ·7H 2 O 0.02%, FeCl 3 ·6H 2 O 0.002%, H 2 O 1000m1.

[0050] The liquid culture medium and fermentation bacteria liquid are prepared by the following method: weigh the above components respectively and dissolve them in 1L of distilled water, adjust the pH to 7.2 with a pH meter after dissolving, put the above solutions in a triangular flask and put them in a sterilized The pot was sterilized at 121°C for 20 minutes. After the culture medium was cooled, 1ml of Bacill...

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Abstract

The invention discloses a method for inhibiting tobacco black shank by combining a bacterial fertilizer with a fermentation bacterial solution, and the method comprises the following steps: (1) preparing the fermentation bacterium solution, (2) preparing the bacterial fertilizer, (3) applying a bottom fertilizer, and (4) pouring the fermentation bacterium solution; according to the method, bacillus cereus is used for preparing the fermentation bacterial solution and the bacterial fertilizer, and the fermentation bacterial solution and the bacterial fertilizer are combined for use, so that thebacillus cereus is applied in a solid state and a liquid state, the bacillus cereus is promoted to be planted in soil and finally forms an advantage flora, and by organic bonding, the fermentation bacterial solution and the bacterial fertilizer have good prevention effect on the tobacco black shank. According to the invention, the use of chemical fertilizers and chemical pesticides is reduced, thegrowth of tobacco is protected, and the environment-friendly effect is promoted.

Description

technical field [0001] The invention belongs to the technical field of biopesticides, and in particular relates to inhibiting the occurrence of tobacco black shank (Phytophthora parasitica var. Background technique [0002] Tobacco black shank (Phytophthora parasitica var.nicotiane) is a devastating disease of tobacco, and it is also one of the most serious diseases in the world's tobacco production. pervasive and severe. In recent years, due to the expansion of the area of ​​continuous cropping tobacco fields and the increase of continuous cropping years, tobacco black shank has become more prevalent in many places. For many years, in order to reduce the losses caused by the disease, various methods such as agricultural control, chemical control, and disease-resistant breeding have been tried to control tobacco black shank. The post-plant incubation period is short, the onset is fast, and re-infection can occur multiple times in a growing season. At the same time, the dis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C05G3/04C05G3/80
Inventor 周晓罡张滨姚春馨余萍丁玉梅杨映平
Owner 云南省农业科学院生物技术与种质资源研究所
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