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Absorbable medical suture and preparation method thereof

A technology of suture and spinning stock solution, which is applied in the field of medical materials, can solve the problems of patients' pain, achieve the effects of alleviating pain, promoting wound healing, and good chemical inertness

Active Publication Date: 2021-06-01
SUZHOU BAIYUAN GENT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Non-absorbable medical sutures need to be removed after surgery, causing pain to patients

Method used

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  • Absorbable medical suture and preparation method thereof
  • Absorbable medical suture and preparation method thereof
  • Absorbable medical suture and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Preparation of modified spidroin protein with amino acid sequence as shown in SEQ ID NO.1

[0031] 1. Determination of MaSp1 gene for artificially synthesized and modified spidroin protein

[0032] The amino acid sequence of the modified spidroin protein selected in this example is shown in SEQ ID NO.1, wherein the amino acid sequence of the protease cleavage site is KKKKSSSSSESSRSSSSSSS (the above letters are common amino acid abbreviations), according to the above selected modified spidroin protein Based on the sequence and the principle of Escherichia coli preferred codon usage, the nucleotide sequence encoding the modified spidroin protein consistent with the amino acid sequence of the modified spidroin protein is designed, see the nucleotide sequence of SEQ ID NO.2 in the sequence listing.

[0033] 2. High-efficiency expression of MaSp1 gene of artificially synthesized and modified spidroin in Escherichia coli

[0034] (1) The MaSp1 gene was synthesized...

experiment example

[0038] The modified spidroin protein obtained in Example 1 was purified respectively, dried, and then subjected to the following tests:

[0039] (1) Toughness and strength testing

[0040] a. Take the dried protein sample and dissolve it with HFIP, the feeding amount is 15% (wt%), stir and dissolve at 60°C for 12h, then rapidly raise the temperature to 120°C and continue stirring for 2h until the solution is uniform and clear, and the spinning stock solution is obtained.

[0041] b. Pour the spinning stock solution into a syringe, extrude the spinning stock solution from a spinneret with an inner diameter of 0.5 mm at a constant spinning speed of 5 ml / min, and spin in a coagulation bath to obtain primary filaments. Each component of the coagulation bath comprises by mass percentage: 4% sodium hydroxide, 4% ethanol, 92% deionized water. image 3 and 4 shown.

[0042] c. Detect the maximum stress (stress (MPa)) and maximum gauge factor (strain (%)) of the fiber and film forme...

Embodiment 2

[0049] In this embodiment, the absorbable medical suture is made of modified spidroin protein, and the amino acid sequence of the modified spidroin protein is shown in SEQ ID NO.1. The preparation of the absorbable medical suture in this embodiment comprises the following steps:

[0050](1) Dissolve 3g of modified spider silk protein in 10ml of 1,1,1,3,3,3-hexafluoroisopropanol solvent, stir and dissolve at 60°C for 12h, then rapidly raise the temperature to 120°C and continue stirring for 2h until the solution is uniform Translucent, the spinning dope is obtained;

[0051] (2) Pour the spinning stock solution into the syringe, and the spinning stock solution is extruded from a spinneret with an inner diameter of 0.5mm at a constant speed of 5ml / min, spinning and forming in the coagulation bath to obtain primary growth filaments, and drying Obtain the absorbable medical suture of present embodiment afterward.

[0052] Each component of the coagulation bath comprises by mass ...

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Abstract

The invention provides an absorbable medical suture and a preparation method thereof in the technical field of medical materials. The raw material of the absorbable medical suture is modified spidroin protein; the amino acid sequence of the modified spidroin protein is shown in SEQ ID NO.1, and the modified spidroin protein has protease cleavage sites KSS, DSS, ESS and RSS , the prepared suture can gradually dissolve in the body, so the prepared suture does not need to be removed after operation, which can reduce the pain of patients; at the same time, because the modified spidroin protein has good biocompatibility and toughness, the prepared suture has good Mechanical properties and biological qualities. The absorbable medical suture raw material provided by the invention also includes hydroxymethyl chitosan, which can promote wound healing. The present invention also provides a preparation method of absorbable medical suture, specifically: dissolving the raw material of absorbable medical suture to obtain spinning stock solution; drying the spinning stock solution after wet spinning to obtain the absorbable medical suture Medical sutures.

Description

technical field [0001] The invention relates to the technical field of medical materials, in particular to an absorbable medical suture and a preparation method thereof. Background technique [0002] According to their biodegradability, medical sutures are divided into non-absorbable medical sutures and absorbable medical sutures. Non-absorbable medical sutures need to be removed after surgery, which brings pain to patients. Absorbable medical sutures are degradable in the body, thereby reducing pain for patients. [0003] At present, the common absorbable medical sutures on the market include polyglycolide sutures, glycolide and lactide sutures, polydioxanone sutures, chitosan sutures, etc. Sutures include polypropylene sutures, polyamide sutures, silk sutures, and the like. [0004] The purpose of the invention is to provide a medical suture material of novel material. Contents of the invention [0005] The invention provides an absorbable medical suture and a prepar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/00A61L17/10A61L17/00
Inventor 李春李琳车锐
Owner SUZHOU BAIYUAN GENT CO LTD
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