MnO2/Fe2O3@ amorphous carbon composite material, aptamer sensor and preparation method and application thereof

A technology of amorphous carbon and composite materials, which is applied to the analysis of materials, electrochemical variables of materials, and material analysis through electromagnetic means, etc. In-situ detection, poor binding activity and other issues, to achieve good electrochemical activity, improve electrochemical activity, and enhance stability

Active Publication Date: 2019-05-28
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a kind of MnO 2 / Fe 2 o 3 @Amorphous carbon composite material preparation method to solve the problem of poor binding activity between the existing substrate material and the nucleic acid aptamer chain, and the nucleic acid aptamer chain is easily detached from the substrate after specific binding to the cancer marker
[0007] The second object of the present invention is to provide the MnO obtained by the above preparation method 2 / Fe 2 o 3 @Amorphous carbon composite material to solve the problem that existing substrate materials cannot effectively adsorb the complex of nucleic acid aptamer chain and cancer marker
[0008] The third object of the present invention is to provide a kind of based on MnO 2 / Fe 2 o 3 @Amorphous carbon composite nucleic acid aptamer sensor preparation method to solve the problem that the existing detection method of tyrosine kinase is not suitable for in situ detection

Method used

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  • MnO2/Fe2O3@ amorphous carbon composite material, aptamer sensor and preparation method and application thereof
  • MnO2/Fe2O3@ amorphous carbon composite material, aptamer sensor and preparation method and application thereof
  • MnO2/Fe2O3@ amorphous carbon composite material, aptamer sensor and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] The preparation method of the nucleic acid aptamer sensor of this embodiment adopts the following steps:

[0075] 1) 118.7mg MnCl 2 4H 2 O, 0.25g Na 3 C 6 h 5 o 7 2H 2 O and 0.3g PVP-K30 were dispersed in 30mL deionized water to form solution A; 66.0mg K 3 [Fe(CN) 6 ] was dispersed in 20 mL deionized water to form solution B. Add solution B to solution A and stir at room temperature for 10 min, then stand at room temperature for 24 h, centrifuge and wash the obtained precipitate 3 times with deionized water, and then vacuum-dry at 60°C to obtain ferromanganese Prussian blue analogue (expressed as MnFe PBA). MnCl 2 4H 2 O, K 3 [Fe(CN) 6 ] The molar ratio is 3:1.

[0076] 2) 130mg FeCl 3 and 40 mg MnFe PBA were dispersed in 5 mL deionized water to form a homogeneous suspension. Then 20 μL of pyrrole was added to the solution, reacted at room temperature for 30 min, and the product was collected by centrifugation, washed several times with ethanol and water...

Embodiment 2

[0082] The preparation method of the nucleic acid aptamer sensor of this embodiment is basically the same as the preparation method of the nucleic acid aptamer sensor Example 1, the only difference is that in step 1), MnCl 2 4H 2 The amount of O used is 79.16 mg, Na 3 C 6 h 5 o 7 2H 2 The dosage of O is 100mg, the dosage of PVP-K30 is 250mg, and the dosage of K 3 [Fe(CN) 6 ] The consumption is 66mg; Step 2) in, FeCl 3 , MnFe PBA, and pyrrole are respectively 48 mg, 40 mg, and 8 μL; in step 3), the calcination temperature is 350° C. and the time is 3 h.

Embodiment 3

[0084] The preparation method of the nucleic acid aptamer sensor of this embodiment is basically the same as the preparation method of the nucleic acid aptamer sensor Example 1, the only difference is that in step 1), MnCl 2 4H 2 The amount of O used is 158.32 mg, Na 3 C 6 h 5 o 7 2H 2 The dosage of O is 500mg, the dosage of PVP-K30 is 500mg, the dosage of K 3 [Fe(CN) 6 ] The consumption is 66mg; Step 2) in, FeCl 3 , MnFe PBA, and pyrrole are 196 mg, 40 mg, and 28 μL. In step 3), the calcination temperature is 450° C. and the time is 1 h.

[0085] The specific examples 1-3 of the nucleic acid aptamer sensor of the present invention respectively correspond to the products obtained in the above examples 1-3 of the preparation method of the nucleic acid aptamer sensor.

[0086] MnO of the present invention 2 / Fe 2 o 3 @Specific examples 1-3 of amorphous carbon composite materials correspond to the products obtained in step 3) of the above nucleic acid aptamer sensor p...

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Abstract

The invention relates to a MnO2 / Fe2O3@ amorphous carbon composite material, an aptamer sensor and a preparation method and application thereof. The preparation method of the composite material comprises the following steps of: 1) conducting a reaction of soluble bivalent manganese salt, soluble ferricyanide and citrate in a solvent to prepare a ferromanganese Prussian blue analog; 2) conducting anoxidative polymerization reaction of the ferromanganese Prussian blue analog, pyrrole and an oxidant in the solvent to obtain a polypyrrole-coated ferromanganese Prussian blue analog; 3) calcining the polypyrrole-coated ferromanganese Prussian blue analog in a protective atmosphere to obtain the composite material. The bimetallic oxide particles and the amorphous carbon in the composite materialprepared by the method have a synergistic effect, so that the electrochemical activity of a substrate material is improved, stronger binding force is applied between the bimetallic oxide particles andan aptamer chain, the stability of G-quadruplexes formed between the aptamer and PTK7 is improved through the biometric identification ability.

Description

technical field [0001] The invention belongs to the field of electrochemical sensors, in particular to a MnO 2 / Fe 2 o 3 @Amorphous carbon composite materials, nucleic acid aptamer sensors and their preparation methods and applications. Background technique [0002] Membrane proteins are essential components of plasma and organelle membranes and also play key roles in many physiological functions, such as ion regulation, energy transfer, molecular recognition, and cellular communication. A large number of human diseases are also closely related to changes in membrane proteins. Protein tyrosine kinase-7 (PTK7) is a membrane protein that plays an important role in the regulation of neural development and planar cell polarity in vertebrates, as well as cell motility during embryonic development. In addition, PTK7 is directly related to human diseases such as acute myeloid leukemia, colon cancer, gastric cancer and lung cancer. Therefore, sensitive and accurate detection of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/30G01N27/38G01N27/48C12N15/115
Inventor 张治红崔静宋英攀王明花何领好胡梦瑶黄顺江
Owner ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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