Improved polyoxin fermentation culture medium and fermentation process

A fermentation medium, polyoxin technology, applied in the direction of fermentation, microorganism-based methods, biochemical equipment and methods, etc., can solve the problem that the viscosity and the elimination of foam during sterilization cannot be scientifically controlled, and there is no special research Over-fermentation medium quality stability, medium quality instability and other issues, to achieve the effect of shortening the fermentation cycle, reducing air demand, and reducing ventilation

Pending Publication Date: 2019-05-28
陕西麦可罗生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The existing polyoxin fermentation process has the following problems: 1. Long-term maintenance of an alkaline pH value environment causes polyoxin to be unstable in an alkaline environment; 2. The viscosity of the medium and the time of sterilization Foam elimination cannot be scientifically controlled during autoclaving of existing media
[0006] But the above prior art all has not specially studied the stable quality problem of fermented medium
In order to improve the quality stability and preparation efficiency of polyoxin fermentation medium, solve the problem of unstable quality of such medium at present, improve the production titer of fermentation, and reduce the difficulty of fermentation filtrate treatment, an improved polyoxin Vegetarian fermentation medium and fermentation process are of great significance

Method used

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  • Improved polyoxin fermentation culture medium and fermentation process
  • Improved polyoxin fermentation culture medium and fermentation process
  • Improved polyoxin fermentation culture medium and fermentation process

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Fermentation medium of the present invention: take by weighing 1.5% of corn flour, 2.0% of soybean cake powder, 2.0% of maltose, KH 2 PO 4 0.1%, NaCl 0.1%, CaCO 3 0.3%, (NH 4 ) 2 SO 4 0.5%, antifoaming agent 0.035%, adjust the pH to 6.5 before adding the antifoaming agent, and sterilize at 121°C for 30min to obtain the fermentation medium of the present invention.

Embodiment 2

[0034] The fermentation medium of Example 1 adopts the fermentation process of the present invention as follows: inoculate the spores of Streptomyces aureus chromogenes slant spores of type 4896 into seed shake flasks at 28° C. and 220 rpm for 30 h, and bottle them into shake flask seeds. The shake flask seeds were inoculated into the primary seed tank at 28°C for 24 hours, transplanted into the sterilized fermentation medium prepared in Example 1, at 28°C, 110rpm, tank pressure 0.05MPa, ventilation rate 0.5-1.0L / min continues to ferment. When cultured to 10h, feed was added (NH 4 ) 2 SO 4 and ammonia water, while adjusting the pH to maintain 6.3-6.5, supplement the nitrogen source, and put the tank into the tank after culturing for 113 hours.

Embodiment 3

[0036] The experimental results of comparative examples 1 and 2 and embodiment 1 and 2 are compared as follows:

[0037] figure 1 It is the original fermentation process using the original fermentation medium, that is, comparative example 2, the figure of the mycelia at 30h, it can be seen that it forms a small net, and the mycelia are long and few. figure 2 For adopting the fermentation process of the fermentation medium of the present invention, i.e. embodiment 2, the figure of mycelium at 30h, it can be seen that it forms a medium net to a large net, and the mycelium is long and complexly cross-linked, with few mycelia and deep staining. .

[0038] image 3 It is the original fermentation process using the original fermentation medium, that is, comparative example 2, the diagram of the hyphae at 68 hours, it can be seen that it forms a large network, and the mycelium is long and complexly cross-linked. Figure 4 For the fermentation process using the fermentation medium...

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Abstract

The invention relates to an improved polyoxin fermentation culture medium and a fermentation process. The polyoxin fermentation culture medium is prepared from the following ingredients: 1% to 2% of maize meal, 1.5% to 3% of soybean cake meal, 1.5% to 3% of cerealose, 0.05% to 0.2% of KH2PO4, 0.05% to 0.2% of NaCl, 0.15% to 0.45% of CaCO3, 0.025% to 0.050% of antifoamer, 0.1% to 2% of (NH4)2SO4andthe balance of aqua sterilisa. According to the improved polyoxin fermentation culture medium and the fermentation process, the fermentation process is optimized, the stability of the fermentation culture medium is improved, and the biological value of the fermentation process is also increased.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to an improved polyoxin fermentation medium and fermentation process. Background technique [0002] Polyoxin (Polyoxin) has a good systemic conduction effect, is a broad-spectrum antibiotic fungicide, is a safe pesticide with high efficiency, low toxicity, and no environmental pollution, so it is widely used in food crops, fruits and vegetables and other important diseases. It is mainly used to control more than ten kinds of crop diseases such as apple leaf spot, rice sheath blight, ring spot, pear black spot, grape gray mold, downy mildew, ginseng black spot and tobacco red spot. [0003] Most of the existing polyoxin raw materials are obtained from the wild-type stock of Streptomyces aureochromogenes No4896 through microbial fermentation, but the fermentation unit is not high and unstable. The technological process of the bacteria fermenting polyoxin i...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P21/02C12R1/465
CPCY02P60/87
Inventor 杨玉旺潘忠成李昶志李蒲民
Owner 陕西麦可罗生物科技有限公司
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