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Azadirachta indica mite and bacterium killing agent and preparation method and application thereof

A fungicide and neem technology, applied in acaricides, botanical equipment and methods, biocides, etc., can solve problems such as human health risks, achieve high-efficiency acaricides and fungicides, reduce impacts, and solve secondary pollution Effect

Inactive Publication Date: 2019-05-28
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These chemical reagent components are used as acaricide preparations, which pose certain risks to human health

Method used

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  • Azadirachta indica mite and bacterium killing agent and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A method for preparing acaricide and fungicide by neem seeds, comprising the steps of:

[0031] Step 1: Wash the neem seeds, dry them at low temperature, and crush them through a 16-mesh sieve;

[0032] Step 2: Put crushed neem seed powder into a hollow glass column, extract 3 times with HPLC grade n-hexane, and remove n-hexane by rotary evaporation at 45°C to obtain substance a;

[0033] Step 3: Use an organic pump to remove the remaining solvent from the substance a obtained in the step 2 to obtain an extract b containing oil-soluble insecticidal active ingredients;

[0034] Step 4: Take the extract b obtained in Step 3 and add a stabilizer for compounding to obtain acaricide and bactericide. The stabilizer includes citric acid, vitamin E and isoascorbic acid.

[0035] The composition of the finally obtained acaricide and fungicide is: 5% citric acid, 5% vitamin E, 5% isoascorbic acid and 85% neem extract.

Embodiment 2

[0037] A method for preparing acaricide and fungicide by neem seeds, comprising the steps of:

[0038] Step 1: Wash the neem seeds, dry them at low temperature, and crush them through a 16-mesh sieve;

[0039] Step 2: put crushed neem seed powder into a hollow glass column, extract 3 times with HPLC grade n-hexane, remove n-hexane by rotary evaporation at 35°C, and obtain substance a;

[0040] Step 3: Use an organic pump to remove the remaining solvent from the substance a obtained in the step 2 to obtain an extract b containing oil-soluble insecticidal active ingredients;

[0041] Step 4: Take the extract b obtained in Step 3 and add a stabilizer for compounding to obtain acaricide and bactericide. The stabilizer includes citric acid, vitamin E and isoascorbic acid.

[0042] The composition of the finally obtained acaricide and fungicide is: 5% citric acid, 5% vitamin E, 5% isoascorbic acid and 85% neem extract.

Embodiment 3

[0044] A method for preparing acaricide and fungicide by neem seeds, comprising the steps of:

[0045] Step 1: Wash the neem seeds, dry them at low temperature, and crush them through a 16-mesh sieve;

[0046] Step 2: put crushed neem seed powder into a hollow glass column, extract 3 times with HPLC grade n-hexane, and remove n-hexane by rotary evaporation at 40°C to obtain substance a;

[0047] Step 3: Use an organic pump to remove the remaining solvent from the substance a obtained in the step 2 to obtain an extract b containing oil-soluble insecticidal active ingredients;

[0048] Step 4: Take the extract b obtained in Step 3 and add a stabilizer for compounding to obtain acaricide and bactericide. The stabilizer includes citric acid, vitamin E and isoascorbic acid.

[0049] The composition of the finally obtained acaricide and fungicide is: 5% citric acid, 5% vitamin E, 5% isoascorbic acid and 85% neem extract.

[0050] The use of neem killing acarids and fungicides prep...

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Abstract

The invention belongs to the field of bactericides, and discloses an azadirachta indica mite and bacterium killing agent and a preparation method and application thereof. The azadirachta indica mite and bacterium killing agent contains an azadirachta indica extract. The preparation method comprises the following steps that step 1, azadirachta indica seed kernels are washed, dried at a low temperature and crushed and sieved by a 16-mesh sieve; step 2, the crushed azadirachta indica seed kernel powder is loaded into a hollow glass column and extracted 3 times with n-hexane, and n-hexane is removed by rotary evaporation under the condition of 40 DEG C to obtain a substance a; and step 3, residual solvents are removed from the substance a by an organic pump to obtain an oil-soluble insecticidal active ingredient to be compounded to obtain the mite and bacterium killing agent. Aiming at the living habit of mites, the azadirachta indica mite and bacterium killing agent adopts the characteristics that natural plant extracts are non-toxic and harmless and has no secondary pollution, thereby achieving the killing of human mites and reducing the impact on human health. The plant extracts adopted have the characteristics such as anti-inflammatory sterilization and no skin allergy after use, and stay on the surface of the skin to kill the mites while preventing the regeneration of the mites.

Description

technical field [0001] The invention belongs to the field of fungicides, and in particular relates to a neem miticide and fungicide as well as its preparation method and application. Background technique [0002] Mites belong to a class of contagious tiny animals belonging to the Arthropoda Arachnida subclass Acari. The body size is generally about 0.5 mm, some are as small as 0.1 mm, and most species are less than 1 mm. There are about 30,000 known species of mites in the world, belonging to 2 orders, 6 suborders, 105 general families, and 380 families, second only to insects. The mites that parasitize the human body are mainly divided into two types, one is hair follicle mite, also called demodex; the other is called sebaceous gland mite, which parasitizes in the sebaceous glands of the human face, and is generally referred to as mite for short. [0003] Mites are widely distributed in the human environment and are closely related to human health. Mites have become anothe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/26A01N25/22A01P7/02A61K36/58A61P33/14
Inventor 刘贝贝吴琳彭黎旭陈歆潘攀符旎晴
Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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