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Method for extracting L-isoleucine by means of chromatography technology

A technology of isoleucine and chromatographic technology, which is applied in the field of extraction of L-isoleucine by chromatographic technology, can solve the problems of high consumption of acid and alkali in elution and regeneration, large amount of exchange resin, high product cost, etc., and achieve the purpose of strengthening CO2 Effects of fixed reaction, increased yield, and low labor intensity

Pending Publication Date: 2019-05-24
XINJIANG FUFENG BIOTECH +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the separation and extraction of L-isoleucine at home and abroad almost all adopt the separation method of ion exchange column. Although this method can be prepared on a large scale, the product cost is high, and the amount of exchange resin is huge, and the acid-base consumption for elution and regeneration is too high. Too many, causing serious environmental pollution, in addition L-isoleucine is easy to lead to low yield and low purity in strong alkali and strong acid environment

Method used

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  • Method for extracting L-isoleucine by means of chromatography technology
  • Method for extracting L-isoleucine by means of chromatography technology

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Experimental program
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Effect test

Embodiment 1

[0029] A method utilizing chromatography to extract L-isoleucine, comprising the steps of:

[0030] Step 1) Cultivate Corynebacterium glutamicum (CGMCC NO.12153, donated by Tianjin University of Science and Technology) to a concentration of 5×10 8 CFU / mL seed liquid, then inoculated into the fermentation medium according to 10% inoculation amount, and fermented for 72 hours. The fermentation culture conditions were: temperature 30°C, dissolved oxygen 30%, pH 6.5-6.8, to obtain L- Isoleucine fermentation broth; the components of the fermentation medium are: glucose 120 g / L, (NH 4 ) 2 SO 4 20g / L, KH 2 PO 4 2.2 g / L, K 2 HPO 4 1.0 g / L, MgSO 4 ·7H 2 O0.4 g / L, FeSO 4 ·7H 2 O 0.015 g / L, MnSO 4 ·H 2 O 0.015 g / L, VH 80 μg / L, VB 1 2.5mg / L, Met30mg / L, Glu 20mg / L, Inositol 20mg / L;

[0031] Step 2) Pass the L-isoleucine fermentation broth through a ceramic membrane (50nm) to remove bacterial protein; the operating temperature of membrane filtration is 50°C, and the workin...

Embodiment 2

[0039]The invention detects the influence of culture medium and culture time on the concentration of L-isoleucine in the fermentation broth.

[0040] Taking Example 1 as the experimental group, set the Glu concentration in the medium to 0, 10, 20, 30, 40, 50 (mg / L), such as figure 1 As shown, with the increase of Glu concentration, the output of L-isoleucine increased significantly, when the Glu concentration increased to 20mg / L, the concentration of L-isoleucine did not change significantly, considering the increase in production and economic factors , choose the most appropriate content of 20mg / L.

[0041] Set the time of fermentation culture as 24, 36, 48, 60, 72, 84 (h), pass figure 2 It can be seen that as the fermentation time increases, the concentration of L-isoleucine increases significantly in the fermentation broth. When the fermentation time reaches 60h, the increase slows down, and the fermentation time reaches the peak at 72h. Continue to increase the fermentat...

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Abstract

The invention belongs to the field of an L-isoleucine extraction technology, and discloses a method for extracting L-isoleucine by means of a chromatographic technology. The method comprises the following steps of fermentation, filtration, chromatographic purification and finished product preparation. Accordingly, the fermentation efficiency is high, L-isoleucine can be thoroughly separated from protein, sugar, inorganic salt and heteroacid in the extraction process, the extraction process is fully automated, the labor intensity is low, the production cost is lowered, and the method conforms to the concept of energy saving and environmental protection.

Description

technical field [0001] The invention belongs to the field of L-isoleucine extraction technology, and specifically provides a method for extracting L-isoleucine by using chromatographic technology. Background technique [0002] L-isoleucine, also known as isoleucine, chemical name L-2-amino-3 methylvaleric acid, in 1901, Fischer discovered optical activity in the L-leucine fraction separated from protein hydrolyzate This is the earliest report about L-isoleucine. L-isoleucine is an important part of living organisms and belongs to essential amino acids, because its special structure and function play an important role in human life metabolism. If the human body lacks L-isoleucine for a long time, it will affect the physiological function of the body, leading to metabolic disorders and decreased resistance. [0003] With its unique efficacy, L-isoleucine is widely used in food health care, biomedicine, medical beauty and other industries. [0004] There are three methods fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C227/40C07C229/08C12P13/06C12R1/15
CPCY02P20/10
Inventor 李德衡王健徐庆阳包鑫刘元涛张宗华杨瑞丽米永花李江雷韦树高
Owner XINJIANG FUFENG BIOTECH
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