Method for detecting organophosphorus pesticides and nitrates

Abstract

The invention discloses a method for detecting organophosphorus pesticides and nitrates. An organophosphorus pesticide rapid detection method based on a silver electrode is established through organophosphorus pesticide rapid detection combining an electrochemical method and an acetylcholinesterase inhibition method. Firstly, a cyclic voltammetry method and a current-time curve method are adopted;the property of the enzymatic reaction product thiocholine is studied, it is found that the silver electrode can generate specific response to thiocholine, electrode passivation and interference areavoided, and real-time monitoring of enzyme activity and pesticide detection are achieved. Linear response range of the silver electrode to thiocholine is 5.2 * 10 <-> 7 to 2.5 * 10<->5 mol / L, whereinthe detection limit of the organophosphorus pesticide (paraoxon) is 6.2 ppb, and the detection recovery rates of the Chinese cabbage and apple samples are 92.05% and 106.11% respectively, so that thesilver electrode is suitable for detecting pesticide residues in agricultural products. And then, a disposable silver electrode is prepared by using a screen printing technology, and the false negative problem of P = S type organophosphorus pesticide detection is solved. The method disclosed by the invention can be used for wearable equipment or directly writing on a monitored object to realize real-time monitoring of tiny angle change, limb actions and even plant behaviors.

Description

technical field [0001] The invention belongs to the field of biology and sex medicine, and specifically relates to the detection technology of biomedicine. Background technique [0002] Existing methods for the detection of organophosphorus pesticides mainly include gas chromatography, mass spectrometry, gas chromatography-mass spectrometry, liquid chromatography-mass spectrometry, high performance liquid chromatography, and Raman spectroscopy. Although these methods have the advantages of accurate quantification and high sensitivity, most of them are large-scale instruments with long detection cycles, which are not suitable for on-site detection and screening of large quantities of samples, and are difficult to meet the prevention and control of emergencies. Contents of the invention [0003] The purpose of the present invention is to provide a detection method for organophosphorus pesticides and nitrates, which can be used on wearable devices or directly written on the m...

AI Technical Summary

Problems solved by technology

Although these methods have the advantages of accurate quantification and high sensitivity, most of them are large-scale instruments with long detection cycles, which are not suitable for on-site detection and screening of large batches of samples, and are difficult to meet the requirements of prevention and control of emergencies.