Liquid fertilizer for promoting rooting and seedling strengthening of tomatoes and preparation method of liquid fertilizer
A technology of liquid fertilizer and tomato, which is applied in the field of liquid fertilizer and its preparation to promote tomato rooting and strong seedlings, to achieve the effects of promoting growth and development, promoting flower and fruit growth, and improving rooting and strong seedlings
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Embodiment 1
[0047] The isolation and identification of embodiment 1 Streptomyces bacterial strain (Streptomyces)
[0048] 1. Experimental materials and conditions
[0049] (1) Soil samples collected in a certain place in Chenzhou City, Hunan Province.
[0050] (2) The components per liter of the liquid medium are as follows: 3.0 g of beef extract, 10.0 g of peptone, 5.0 g of sodium chloride, dilute to 1 liter of water, and the pH is 7.5.
[0051] The components per liter of solid medium are as follows: 3.0g of beef extract, 10.0g of peptone, 5.0g of sodium chloride, 15-25g of agar, dilute to 1 liter of water, and the pH is 7.5.
[0052] The culture medium was divided into aliquots and sterilized in an autoclave (121°C, 30min).
[0053] (3) Sterile operating conditions: All utensils and utensils must be sterilized in an autoclave (121°C, 30min), and inoculation and other operations are carried out in an ultra-clean workbench.
[0054] (4) Conditions for culturing strains: place them in ...
Embodiment 2
[0069] Embodiment 2 Preparation and application of a liquid fertilizer for promoting tomato rooting and strong seedlings
[0070] A kind of preparation method of the liquid fertilizer that promotes tomato rooting and strong seedling comprises the following steps:
[0071] S1. Preparation of culture medium: soak 1000g of soybeans for 10 hours, put them into a refiner and grind them into soybean milk, filter out the bean dregs, put the filtrate in a clean fermenter, add white sugar to the filtrate, add water to The concentration of sugar in the medium is 100g / L, and the pH is 7.5;
[0072] S2. inoculation: get the liquid spawn of Streptomyces bacterial strain " TY-001# ", inoculate in the culture medium, stir evenly; There are 200,000,000 viable bacteria per milliliter in the liquid spawn; The ratio of liquid spawn and culture medium 165g:250L;
[0073] S3. Fermentation: Turn on the oxygen pump, continuously feed oxygen into the fermenter during the fermentation process, and a...
Embodiment 3
[0080] Embodiment 3 A kind of preparation and application of the liquid fertilizer that promotes tomato rooting and strong seedling
[0081] A kind of preparation method of the liquid fertilizer that promotes tomato rooting and strong seedling comprises the following steps:
[0082] S1. Preparation of medium: take 1000g of soybean meal for pre-fermentation, put it into a refiner for beating, filter, put the filtrate in a clean fermentation barrel, then add white sugar to the filtrate, add water to the medium The concentration of medium sugar is 100g / L, and the pH is 7.5;
[0083] The steps of pre-fermentation are:
[0084] S11. Add water to the soybean meal, and stir well and evenly until the soybean meal is squeezed tightly by hand to reach the state of dripping water;
[0085] S12. Cover the surface of the soybean meal with a film, leaving no gaps, and start the pre-fermentation process by timing;
[0086] S13. The pre-fermentation time is 4 days; 2 days after the start o...
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