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Liquid fertilizer for promoting rooting and seedling strengthening of Cuiguan pear and preparation method of liquid fertilizer

A liquid fertilizer and a liquid technology are applied in the field of liquid fertilizer for promoting the rooting and strengthening of seedlings of Cuiguan pear and the field of preparation thereof, so as to achieve the effects of improving root system growth, being beneficial to quality and promoting rooting and strengthening of seedlings.

Inactive Publication Date: 2018-12-07
郴州市通源生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to overcome the problem of the root growth of the existing Cuiguan pear, and to provide a liquid fertilizer for promoting the rooting and strong seedlings of Cuiguan pear and its preparation method

Method used

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  • Liquid fertilizer for promoting rooting and seedling strengthening of Cuiguan pear and preparation method of liquid fertilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Isolation and identification of Streptomyces strains

[0046] 1. Experimental materials and conditions

[0047] (1) A soil sample collected in a place in Chenzhou City, Hunan Province.

[0048] (2) The components per liter of liquid culture medium are as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, the volume of water is fixed to 1 liter, and the pH is 7.4-7.6.

[0049] The components per liter of the solid medium are as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, agar 15-25g, the volume of water is fixed to 1 liter, and the pH is 7.4-7.6.

[0050] The culture medium is subpackaged and sterilized in an autoclave (121°C, 30min).

[0051] (3) Aseptic operating conditions: All utensils and utensils must be sterilized by an autoclave (121°C, 30min), and operations such as inoculation are performed in an ultra-clean workbench.

[0052] (4) Strain culture conditions: culture in a light incubator (14L: 10D) at 25±1°C. After colonies are f...

Embodiment 2

[0067] Example 2 Preparation and application of a liquid fertilizer for promoting rooting and strong seedlings of Cuiguan pear

[0068] A preparation method of liquid fertilizer for promoting rooting and strong seedlings of Cuiguan pear includes the following steps:

[0069] S1. Preparation of culture medium: After soaking 1000g soybeans for 10 hours, put them in a refiner and grind them into soybean milk, filter off the bean dregs, put the filtrate in a clean fermentation tank, and then add white sugar to the filtrate, and add water to make the volume up The concentration of sugar in the medium is 100g / L and the pH is 7.4~7.6;

[0070] S2. Inoculation: Take the liquid strain of Streptomyces strain "TY-001#", inoculate it into the culture medium, and stir evenly; there are 200 million live bacteria per milliliter in the liquid strain; the ratio of the liquid strain to the medium 165g: 250L

[0071] S3. Fermentation: Turn on the oxygen pump, continue to inject oxygen into the fermenta...

Embodiment 3

[0077] Example 3 Preparation and application of a liquid fertilizer for promoting rooting and strong seedlings of Cuiguan pear

[0078] A preparation method of liquid fertilizer for promoting rooting and strong seedlings of Cuiguan pear includes the following steps:

[0079] S1. Preparation of culture medium: take 1000g soybean meal for pre-fermentation, put it into a refiner for beating, filter, put the filtrate in a clean fermentation tank, and then add white sugar to the filtrate, and add water to the culture medium. The concentration of medium sugar is 100g / L, and the pH is 7.4~7.6;

[0080] The steps of pre-fermentation are:

[0081] S11. Add water to the soybean meal, and stir it evenly until the soybean meal is pinched by hand to a dripping state;

[0082] S12. Cover the surface of soybean meal with a film, leaving no gaps, and start the pre-fermentation process at a timing;

[0083] S13. The pre-fermentation time is 4 days; 2 days after the start of fermentation, insert the ther...

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Abstract

The invention discloses a liquid fertilizer for promoting rooting and seedling strengthening of Cuiguan pear and a preparation method of the liquid fertilizer. The liquid fertilizer comprises 1000 parts of an active liquid fertilizer substrate preparation, 15-30 parts of nitrogen, 6-10 parts of potassium, 2-6 parts of phosphorus, 10-20 parts of boron and 12-22 parts of zinc. The liquid fertilizercontains 10-15 parts of proline, 5-10 parts of phenylalanine, 3-5 parts of glutamic acid, 12-16 parts of serine, 7-9 parts of glycine, 4-6 parts of alanine, 13-17 parts of leucine and 40-50 parts of aspartic acid. After the liquid fertilizer is applied, the total root number of the Cuiguan pear is increased by 33.8%, and the root amount, the root distribution and the root composition are obviouslyimproved relative to a control group. Compared with the growing state in the control group, the tree height is increased by 21.6%, the crown diameter is increased by 29.1%, the number of new shoots is increased by 36.2%, the leaf color of a tree body is dark green, and the content of chlorophyll is obviously increased.

Description

Technical field [0001] The invention belongs to the technical field of liquid fertilizers, and more specifically, relates to a liquid fertilizer for promoting rooting and strong seedlings of Cuiguan pear and a preparation method thereof. Background technique [0002] Cuiguan pear is a honey pear, also known as "June Xue". It has a thin skin, crispy flesh and juicy, rich and tender flavor, and sweet juicy flavor. Cuiguan pear belongs to the sand pear series, the fruit is nearly round, the fruit shape index is 0.96, the soluble solid content is 12% to 14%, yellow-green, snow-white flesh, and tender flesh. The weight of a single fruit is 200 grams, and the maximum is about 500 grams. The edible rate of the fruit is 96%. It will be on the market in early and mid-July. The fruit is oblate, the surface is clean, the flesh is white, the core is small and the flesh is thick and tender, and the juice is rich and sweet. With honey fragrance, it has a special taste, and has the reputation ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C05F17/00C12N1/20C12R1/465
CPCC05C11/00C05F17/00C12N1/20C05G5/20C05D1/00C05D9/02C05F11/08Y02W30/40
Inventor 盛建平张利军李国恒欧琴华张燕梅
Owner 郴州市通源生物科技有限公司
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